Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector
Plant reoviruses are able to multiply in gramineae plants and delphacid vectors encountering different defense strategies with unique features. This study aims to comparatively assess alterations of small RNA (sRNA) populations in both hosts upon virus infection. For this purpose, we characterized...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1108 http://journal.frontiersin.org/article/10.3389/fpls.2017.00766/full https://doi.org/10.3389/fpls.2017.00766 |
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| author | De Haro, Luis Alejandro Dumon, Analia Delina Mattio, Maria Fernanda Arguello Caro, Evangelina Beatriz Llauger, Gabriela Zavallo, Diego Blanc, Hervé Mongelli, Vanesa Claudia Truol, Graciela Ana Maria Saleh, María-Carla Asurmendi, Sebastian Del Vas, Mariana |
| author_browse | Arguello Caro, Evangelina Beatriz Asurmendi, Sebastian Blanc, Hervé De Haro, Luis Alejandro Del Vas, Mariana Dumon, Analia Delina Llauger, Gabriela Mattio, Maria Fernanda Mongelli, Vanesa Claudia Saleh, María-Carla Truol, Graciela Ana Maria Zavallo, Diego |
| author_facet | De Haro, Luis Alejandro Dumon, Analia Delina Mattio, Maria Fernanda Arguello Caro, Evangelina Beatriz Llauger, Gabriela Zavallo, Diego Blanc, Hervé Mongelli, Vanesa Claudia Truol, Graciela Ana Maria Saleh, María-Carla Asurmendi, Sebastian Del Vas, Mariana |
| author_sort | De Haro, Luis Alejandro |
| collection | INTA Digital |
| description | Plant reoviruses are able to multiply in gramineae plants and delphacid vectors encountering different defense strategies with unique features. This study aims to
comparatively assess alterations of small RNA (sRNA) populations in both hosts upon virus infection. For this purpose, we characterized the sRNA profiles of wheat and planthopper vectors infected by Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) and quantified virus genome segments by quantitative reverse transcription PCR We provide evidence that plant and insect silencing machineries differentially recognize the viral genome, thus giving rise to distinct profiles of virus-derived small interfering RNAs (vsiRNAs). In plants, most of the virus genome segments were targeted preferentially within their upstream sequences and vsiRNAs mapped with higher density to the smaller genome segments than to the medium or larger ones. This tendency, however, was not observed in insects. In both hosts, vsiRNAs were equally derived from sense and antisense RNA strands and the differences in vsiRNAs accumulation did not correlate with mRNAs accumulation. We also established that the piwi-interacting RNA (piRNA) pathway was active in the delphacid vector but, contrary to what is observed in virus infected mosquitoes, virus-specific piRNAs were not detected. This work contributes to the understanding of the silencing response in insect and plant hosts. |
| format | Artículo |
| id | INTA1108 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA11082017-12-15T18:42:10Z Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector De Haro, Luis Alejandro Dumon, Analia Delina Mattio, Maria Fernanda Arguello Caro, Evangelina Beatriz Llauger, Gabriela Zavallo, Diego Blanc, Hervé Mongelli, Vanesa Claudia Truol, Graciela Ana Maria Saleh, María-Carla Asurmendi, Sebastian Del Vas, Mariana ARN Virus de las Plantas Trigo Fulgoroidea Plant Viruses Wheat RNA Virus del Mal de Río Cuarto Fijivirus Plant reoviruses are able to multiply in gramineae plants and delphacid vectors encountering different defense strategies with unique features. This study aims to comparatively assess alterations of small RNA (sRNA) populations in both hosts upon virus infection. For this purpose, we characterized the sRNA profiles of wheat and planthopper vectors infected by Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) and quantified virus genome segments by quantitative reverse transcription PCR We provide evidence that plant and insect silencing machineries differentially recognize the viral genome, thus giving rise to distinct profiles of virus-derived small interfering RNAs (vsiRNAs). In plants, most of the virus genome segments were targeted preferentially within their upstream sequences and vsiRNAs mapped with higher density to the smaller genome segments than to the medium or larger ones. This tendency, however, was not observed in insects. In both hosts, vsiRNAs were equally derived from sense and antisense RNA strands and the differences in vsiRNAs accumulation did not correlate with mRNAs accumulation. We also established that the piwi-interacting RNA (piRNA) pathway was active in the delphacid vector but, contrary to what is observed in virus infected mosquitoes, virus-specific piRNAs were not detected. This work contributes to the understanding of the silencing response in insect and plant hosts. Inst. de Biotecnología Fil: De Haro, Luis Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dumon, Analia Delina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Mattio, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Arguello Caro, Evangelina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Llauger, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Blanc, Hervé. Centre National de la Recherche Scientifique. Institut Pasteur, Viruses and RNA Interference Unit; Francia Fil: Truol, Graciela Ana Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Mongelli, Vanesa Claudia. Centre National de la Recherche Scientifique. Institut Pasteur, Viruses and RNA Interference Unit; Francia Fil: Saleh, María-Carla. Centre National de la Recherche Scientifique. Institut Pasteur, Viruses and RNA Interference Unit; Francia Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-09-01T18:02:06Z 2017-09-01T18:02:06Z 2017 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1108 http://journal.frontiersin.org/article/10.3389/fpls.2017.00766/full de Haro LA, Dumón AD, Mattio MF, Argüello Caro EB, Llauger G, Zavallo D, Blanc H, Mongelli VC, Truol G, Saleh M-C, Asurmendi S and del Vas M (2017) Mal de Río Cuarto Virus Infection Triggers the Production of Distinctive Viral-Derived siRNA Profiles in Wheat and Its Planthopper Vector. Front. Plant Sci. 8:766 https://doi.org/10.3389/fpls.2017.00766 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers in plant science 8 :766. (May 2017) |
| spellingShingle | ARN Virus de las Plantas Trigo Fulgoroidea Plant Viruses Wheat RNA Virus del Mal de Río Cuarto Fijivirus De Haro, Luis Alejandro Dumon, Analia Delina Mattio, Maria Fernanda Arguello Caro, Evangelina Beatriz Llauger, Gabriela Zavallo, Diego Blanc, Hervé Mongelli, Vanesa Claudia Truol, Graciela Ana Maria Saleh, María-Carla Asurmendi, Sebastian Del Vas, Mariana Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector |
| title | Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector |
| title_full | Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector |
| title_fullStr | Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector |
| title_full_unstemmed | Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector |
| title_short | Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector |
| title_sort | mal de rio cuarto virus infection triggers the production of distinctive viral derived sirna profiles in wheat and its planthopper vector |
| topic | ARN Virus de las Plantas Trigo Fulgoroidea Plant Viruses Wheat RNA Virus del Mal de Río Cuarto Fijivirus |
| url | http://hdl.handle.net/20.500.12123/1108 http://journal.frontiersin.org/article/10.3389/fpls.2017.00766/full https://doi.org/10.3389/fpls.2017.00766 |
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