Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940
A main goal of biological nitrogen fixation research has been to expand the nitrogen-fixing ability to major cereal crops. In this work, we demonstrate the use of the efficient nitrogen-fixing rhizobacterium Pseudomonas protegens Pf-5 X940 as a chassis to engineer the transfer of nitrogen fixed by...
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| Format: | info:eu-repo/semantics/article |
| Language: | Inglés |
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2017
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/1105 http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13376/epdf?r3_referer=wol&tracking_action=preview_click&show_checkout=1&purchase_referrer=onlinelibrary.wiley.com&purchase_site_license=LICENSE_DENIED |
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| author | Fox, Ana Romina Soto, Gabriela Cinthia Valverde, Claudio Fabián Russo, Daniela Marta Lagares, Antonio Zorreguieta, Ángeles Pascuan, Cecilia Gabriela Frare, Romina Alejandra Mercado Blanco, Jesús Dixon, Ray Ayub, Nicolás Daniel Alleva, Karina Edith |
| author_browse | Alleva, Karina Edith Ayub, Nicolás Daniel Dixon, Ray Fox, Ana Romina Frare, Romina Alejandra Lagares, Antonio Mercado Blanco, Jesús Pascuan, Cecilia Gabriela Russo, Daniela Marta Soto, Gabriela Cinthia Valverde, Claudio Fabián Zorreguieta, Ángeles |
| author_facet | Fox, Ana Romina Soto, Gabriela Cinthia Valverde, Claudio Fabián Russo, Daniela Marta Lagares, Antonio Zorreguieta, Ángeles Pascuan, Cecilia Gabriela Frare, Romina Alejandra Mercado Blanco, Jesús Dixon, Ray Ayub, Nicolás Daniel Alleva, Karina Edith |
| author_sort | Fox, Ana Romina |
| collection | INTA Digital |
| description | A main goal of biological nitrogen fixation research has been to expand the nitrogen-fixing ability to major cereal crops. In this work, we demonstrate the
use of the efficient nitrogen-fixing rhizobacterium Pseudomonas protegens Pf-5 X940 as a chassis to engineer the transfer of nitrogen fixed by BNF to maize and wheat under non-gnotobiotic conditions.
Inoculation of maize and wheat with Pf-5 X940 largely improved nitrogen content and biomass accumulation in both vegetative and reproductive tissues, and this beneficial effect was positively associated with high nitrogen fixation rates in roots. 15N isotope dilution analysis showed that maize and wheat plants obtained substantial amounts of fixed nitrogen from the atmosphere. Pf-5 X940-GFP-tagged cells were always reisolated from the maize and wheat root surface but never from the inner root tissues. Confocal laser scanning microscopy confirmed root surface colonization of Pf-5 X940-GFP in wheat plants, and microcolonies were mostly visualized at the junctions between epidermal root cells. Genetic analysis using biofilm formation-related Pseudomonas mutants confirmed
the relevance of bacterial root adhesion in the increase in nitrogen content, biomass accumulation and nitrogen fixation rates in wheat roots. To our
knowledge, this is the first report of robust BNF in major cereal crops. |
| format | info:eu-repo/semantics/article |
| id | INTA1105 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA11052017-12-29T15:15:09Z Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940 Fox, Ana Romina Soto, Gabriela Cinthia Valverde, Claudio Fabián Russo, Daniela Marta Lagares, Antonio Zorreguieta, Ángeles Pascuan, Cecilia Gabriela Frare, Romina Alejandra Mercado Blanco, Jesús Dixon, Ray Ayub, Nicolás Daniel Alleva, Karina Edith Fijación Biológica del Nitrógeno Cultivos de Cereales Bacteria Fijadora del Nitrógeno Pseudomonas Biological Nitrogen Fixation Cereal Crops Nitrogen Fixing Bacteria A main goal of biological nitrogen fixation research has been to expand the nitrogen-fixing ability to major cereal crops. In this work, we demonstrate the use of the efficient nitrogen-fixing rhizobacterium Pseudomonas protegens Pf-5 X940 as a chassis to engineer the transfer of nitrogen fixed by BNF to maize and wheat under non-gnotobiotic conditions. Inoculation of maize and wheat with Pf-5 X940 largely improved nitrogen content and biomass accumulation in both vegetative and reproductive tissues, and this beneficial effect was positively associated with high nitrogen fixation rates in roots. 15N isotope dilution analysis showed that maize and wheat plants obtained substantial amounts of fixed nitrogen from the atmosphere. Pf-5 X940-GFP-tagged cells were always reisolated from the maize and wheat root surface but never from the inner root tissues. Confocal laser scanning microscopy confirmed root surface colonization of Pf-5 X940-GFP in wheat plants, and microcolonies were mostly visualized at the junctions between epidermal root cells. Genetic analysis using biofilm formation-related Pseudomonas mutants confirmed the relevance of bacterial root adhesion in the increase in nitrogen content, biomass accumulation and nitrogen fixation rates in wheat roots. To our knowledge, this is the first report of robust BNF in major cereal crops. Inst. de Genética "Ewald A. Favret"- IGEAF Fil: Fox, Ana Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Valverde, Claudio Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina Fil: Russo, Daniela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Lagares, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina Fil: Zorreguieta, Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Alleva, Karina Edith. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mercado Blanco, Jesús. Consejo Superior de Investigaciones Científicas. Instituto de Agricultura Sostenible. Departamento de Protección de Cultivos; España Fil: Dixon, Ray. John Innes Centre. Department of Molecular Microbiology; Reino Unido Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-09-01T14:40:07Z 2017-09-01T14:40:07Z 2016 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:ar-repo/semantics/artículo http://hdl.handle.net/20.500.12123/1105 http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13376/epdf?r3_referer=wol&tracking_action=preview_click&show_checkout=1&purchase_referrer=onlinelibrary.wiley.com&purchase_site_license=LICENSE_DENIED 1462-2920 (Online) DOI: 10.1111/1462-2920.13376 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Environmental microbiology 18 (10) : 3522–3534. (October 2016) |
| spellingShingle | Fijación Biológica del Nitrógeno Cultivos de Cereales Bacteria Fijadora del Nitrógeno Pseudomonas Biological Nitrogen Fixation Cereal Crops Nitrogen Fixing Bacteria Fox, Ana Romina Soto, Gabriela Cinthia Valverde, Claudio Fabián Russo, Daniela Marta Lagares, Antonio Zorreguieta, Ángeles Pascuan, Cecilia Gabriela Frare, Romina Alejandra Mercado Blanco, Jesús Dixon, Ray Ayub, Nicolás Daniel Alleva, Karina Edith Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940 |
| title | Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940 |
| title_full | Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940 |
| title_fullStr | Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940 |
| title_full_unstemmed | Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940 |
| title_short | Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940 |
| title_sort | major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen fixing bacterium pseudomonas protegens pf 5 x940 |
| topic | Fijación Biológica del Nitrógeno Cultivos de Cereales Bacteria Fijadora del Nitrógeno Pseudomonas Biological Nitrogen Fixation Cereal Crops Nitrogen Fixing Bacteria |
| url | http://hdl.handle.net/20.500.12123/1105 http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13376/epdf?r3_referer=wol&tracking_action=preview_click&show_checkout=1&purchase_referrer=onlinelibrary.wiley.com&purchase_site_license=LICENSE_DENIED |
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