Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)
Background: Leaf senescence delay impacts positively in grain yield by maintaining the photosynthetic area during the reproductive stage and during grain filling. Therefore a comprehensive understanding of the gene families associated with leaf senescence is essential. NAC transcription factors (TF)...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/11022 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-08199-5 https://doi.org/10.1186/s12864-021-08199-5 |
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| author | Bengoa Luoni, Sofía Ailin Cenci, Alberto Moschen, Sebastian Nicolas Nicosia, Salvador Maria Radonic, Laura Mabel Sabio Y Garcia, Julia Veronica Langlade, Nicolas B. Vile, Denis Vazquez Rovere, Cecilia Fernandez, Paula Del Carmen |
| author_browse | Bengoa Luoni, Sofía Ailin Cenci, Alberto Fernandez, Paula Del Carmen Langlade, Nicolas B. Moschen, Sebastian Nicolas Nicosia, Salvador Maria Radonic, Laura Mabel Sabio Y Garcia, Julia Veronica Vazquez Rovere, Cecilia Vile, Denis |
| author_facet | Bengoa Luoni, Sofía Ailin Cenci, Alberto Moschen, Sebastian Nicolas Nicosia, Salvador Maria Radonic, Laura Mabel Sabio Y Garcia, Julia Veronica Langlade, Nicolas B. Vile, Denis Vazquez Rovere, Cecilia Fernandez, Paula Del Carmen |
| author_sort | Bengoa Luoni, Sofía Ailin |
| collection | INTA Digital |
| description | Background: Leaf senescence delay impacts positively in grain yield by maintaining the photosynthetic area during the reproductive stage and during grain filling. Therefore a comprehensive understanding of the gene families associated with leaf senescence is essential. NAC transcription factors (TF) form a large plant-specific gene family involved in regulating development, senescence, and responses to biotic and abiotic stresses. The main goal of this work was to identify sunflower NAC TF (HaNAC) and their association with senescence, studying their orthologous to understand possible functional relationships between genes of different species.
Results: To clarify the orthologous relationships, we used an in-depth comparative study of four divergent taxa, in dicots and monocots, with completely sequenced genomes (Arabidopsis thaliana, Vitis vinifera, Musa acuminata and Oryza sativa). These orthologous groups provide a curated resource for large scale protein sequence annotation of NAC TF. From the 151 HaNAC genes detected in the latest version of the sunflower genome, 50 genes were associated with senescence traits. These genes showed significant differential expression in two contrasting lines according to an RNAseq assay. An assessment of overexpressing the Arabidopsis line for HaNAC001 (a gene of the same orthologous group of Arabidopsis thaliana ORE1) revealed that this line displayed a significantly higher number of senescent leaves and a pronounced change in development rate.
Conclusions: This finding suggests HaNAC001 as an interesting candidate to explore the molecular regulation of senescence in sunflower. |
| format | info:ar-repo/semantics/artículo |
| id | INTA11022 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA110222021-12-30T12:39:44Z Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus) Bengoa Luoni, Sofía Ailin Cenci, Alberto Moschen, Sebastian Nicolas Nicosia, Salvador Maria Radonic, Laura Mabel Sabio Y Garcia, Julia Veronica Langlade, Nicolas B. Vile, Denis Vazquez Rovere, Cecilia Fernandez, Paula Del Carmen Helianthus annuus Avejentamiento Genética Factores de Transcripción Análisis Filogenético Senescence Genetics Transcription Factors Phylogenetic Analysis Leaves Hojas Girasol Sunflower Leaf Senescence Background: Leaf senescence delay impacts positively in grain yield by maintaining the photosynthetic area during the reproductive stage and during grain filling. Therefore a comprehensive understanding of the gene families associated with leaf senescence is essential. NAC transcription factors (TF) form a large plant-specific gene family involved in regulating development, senescence, and responses to biotic and abiotic stresses. The main goal of this work was to identify sunflower NAC TF (HaNAC) and their association with senescence, studying their orthologous to understand possible functional relationships between genes of different species. Results: To clarify the orthologous relationships, we used an in-depth comparative study of four divergent taxa, in dicots and monocots, with completely sequenced genomes (Arabidopsis thaliana, Vitis vinifera, Musa acuminata and Oryza sativa). These orthologous groups provide a curated resource for large scale protein sequence annotation of NAC TF. From the 151 HaNAC genes detected in the latest version of the sunflower genome, 50 genes were associated with senescence traits. These genes showed significant differential expression in two contrasting lines according to an RNAseq assay. An assessment of overexpressing the Arabidopsis line for HaNAC001 (a gene of the same orthologous group of Arabidopsis thaliana ORE1) revealed that this line displayed a significantly higher number of senescent leaves and a pronounced change in development rate. Conclusions: This finding suggests HaNAC001 as an interesting candidate to explore the molecular regulation of senescence in sunflower. Instituto de Biotecnología Fil: Bengoa Luoni, Sofia Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Cenci, Alberto. Bioversity International; Francia Fil: Moschen, Sebastian Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina. Fil: Nicosia, Salvador. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Radonic, Laura Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Langlade, Nicolas B. Université de Toulouse. LIPM-INRA-CNRS; Francia Fil: Langlade, Nicolas B. Université de Montpellier. LEPSE; Francia Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. 2021-12-30T12:35:10Z 2021-12-30T12:35:10Z 2021-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11022 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-08199-5 1471-2164 https://doi.org/10.1186/s12864-021-08199-5 eng info:eu-repograntAgreement/INTA/PNBIO-1131022/AR./Genómica funcional y biología de sistemas. info:eu-repograntAgreement/INTA/PNBIO-1131043/AR./Bioinformática y Estadística Genómica. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer BMC Genomics 22 : Article number: 893 (2021) |
| spellingShingle | Helianthus annuus Avejentamiento Genética Factores de Transcripción Análisis Filogenético Senescence Genetics Transcription Factors Phylogenetic Analysis Leaves Hojas Girasol Sunflower Leaf Senescence Bengoa Luoni, Sofía Ailin Cenci, Alberto Moschen, Sebastian Nicolas Nicosia, Salvador Maria Radonic, Laura Mabel Sabio Y Garcia, Julia Veronica Langlade, Nicolas B. Vile, Denis Vazquez Rovere, Cecilia Fernandez, Paula Del Carmen Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus) |
| title | Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus) |
| title_full | Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus) |
| title_fullStr | Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus) |
| title_full_unstemmed | Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus) |
| title_short | Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus) |
| title_sort | genome wide and comparative phylogenetic analysis of senescence associated nac transcription factors in sunflower helianthus annuus |
| topic | Helianthus annuus Avejentamiento Genética Factores de Transcripción Análisis Filogenético Senescence Genetics Transcription Factors Phylogenetic Analysis Leaves Hojas Girasol Sunflower Leaf Senescence |
| url | http://hdl.handle.net/20.500.12123/11022 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-08199-5 https://doi.org/10.1186/s12864-021-08199-5 |
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