Genomic analysis of shiga toxin-containing Escherichia coli O157:H7 isolated from Argentinean cattle
Cattle are the main reservoir of Enterohemorrhagic Escherichia coli (EHEC), with O157:H7 the distinctive serotype. EHEC is the main causative agent of a severe systemic disease, Hemolytic Uremic Syndrome (HUS). Argentina has the highest pediatric HUS incidence worldwide with 12–14 cases per 100,000...
| Main Authors: | , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Plos
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/11003 https://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0258753 https://doi.org/10.1371/journal.pone.0258753 |
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| author | Amadio, Ariel Bono, James L. Irazoqui, Jose Matias Larzabal, Mariano Marques Da Silva, Wanderson Eberhardt, María Florencia Riviere, Nahuel Agustin Gally, David Manning, Shannon D. Cataldi, Angel Adrian |
| author_browse | Amadio, Ariel Bono, James L. Cataldi, Angel Adrian Eberhardt, María Florencia Gally, David Irazoqui, Jose Matias Larzabal, Mariano Manning, Shannon D. Marques Da Silva, Wanderson Riviere, Nahuel Agustin |
| author_facet | Amadio, Ariel Bono, James L. Irazoqui, Jose Matias Larzabal, Mariano Marques Da Silva, Wanderson Eberhardt, María Florencia Riviere, Nahuel Agustin Gally, David Manning, Shannon D. Cataldi, Angel Adrian |
| author_sort | Amadio, Ariel |
| collection | INTA Digital |
| description | Cattle are the main reservoir of Enterohemorrhagic Escherichia coli (EHEC), with O157:H7 the distinctive serotype. EHEC is the main causative agent of a severe systemic disease, Hemolytic Uremic Syndrome (HUS). Argentina has the highest pediatric HUS incidence worldwide with 12–14 cases per 100,000 children. Herein, we assessed the genomes of EHEC O157:H7 isolates recovered from cattle in the humid Pampas of Argentina. According to phylogenetic studies, EHEC O157 can be divided into clades. Clade 8 strains that were classified as hypervirulent. Most of the strains of this clade have a Shiga toxin stx2a-stx2c genotype. To better understand the molecular bases related to virulence, pathogenicity and evolution of EHEC O157:H7, we performed a comparative genomic analysis of these isolates through whole genome sequencing. The isolates classified as clade 8 (four strains) and clade 6 (four strains) contained 13 to 16 lambdoid prophages per genome, and the observed variability of prophages was analysed. An inter strain comparison show that while some prophages are highly related and can be grouped into families, other are unique. Prophages encoding for stx2a were highly diverse, while those encoding for stx2c were conserved. A cluster of genes exclusively found in clade 8 contained 13 genes that mostly encoded for DNA binding proteins. In the studied strains, polymorphisms in Q antiterminator, the Q-stx2A intergenic region and the O and P γ alleles of prophage replication proteins are associated with different levels of Stx2a production. As expected, all strains had the pO157 plasmid that was highly conserved, although one strain displayed a transposon interruption in the protease EspP gene. This genomic analysis may contribute to the understanding of the genetic basis of the hypervirulence of EHEC O157:H7 strains circulating in Argentine cattle. This work aligns with other studies of O157 strain variation in other populations that shows key differences in Stx2a-encoding prophages. |
| format | Artículo |
| id | INTA11003 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Plos |
| publisherStr | Plos |
| record_format | dspace |
| spelling | INTA110032021-12-28T13:19:21Z Genomic analysis of shiga toxin-containing Escherichia coli O157:H7 isolated from Argentinean cattle Amadio, Ariel Bono, James L. Irazoqui, Jose Matias Larzabal, Mariano Marques Da Silva, Wanderson Eberhardt, María Florencia Riviere, Nahuel Agustin Gally, David Manning, Shannon D. Cataldi, Angel Adrian Ganado Bovino Escherichia coli Toxinas Enfermedades de los Animales Genética Molecular Argentina Cattle Toxins Animal Diseases Molecular Genetics Toxina Shiga Shiga Toxin Cattle are the main reservoir of Enterohemorrhagic Escherichia coli (EHEC), with O157:H7 the distinctive serotype. EHEC is the main causative agent of a severe systemic disease, Hemolytic Uremic Syndrome (HUS). Argentina has the highest pediatric HUS incidence worldwide with 12–14 cases per 100,000 children. Herein, we assessed the genomes of EHEC O157:H7 isolates recovered from cattle in the humid Pampas of Argentina. According to phylogenetic studies, EHEC O157 can be divided into clades. Clade 8 strains that were classified as hypervirulent. Most of the strains of this clade have a Shiga toxin stx2a-stx2c genotype. To better understand the molecular bases related to virulence, pathogenicity and evolution of EHEC O157:H7, we performed a comparative genomic analysis of these isolates through whole genome sequencing. The isolates classified as clade 8 (four strains) and clade 6 (four strains) contained 13 to 16 lambdoid prophages per genome, and the observed variability of prophages was analysed. An inter strain comparison show that while some prophages are highly related and can be grouped into families, other are unique. Prophages encoding for stx2a were highly diverse, while those encoding for stx2c were conserved. A cluster of genes exclusively found in clade 8 contained 13 genes that mostly encoded for DNA binding proteins. In the studied strains, polymorphisms in Q antiterminator, the Q-stx2A intergenic region and the O and P γ alleles of prophage replication proteins are associated with different levels of Stx2a production. As expected, all strains had the pO157 plasmid that was highly conserved, although one strain displayed a transposon interruption in the protease EspP gene. This genomic analysis may contribute to the understanding of the genetic basis of the hypervirulence of EHEC O157:H7 strains circulating in Argentine cattle. This work aligns with other studies of O157 strain variation in other populations that shows key differences in Stx2a-encoding prophages. EEA Rafaela Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Amadio, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Bono, James L. U.S. Department of Agriculture. Agricultural Research Service. U.S Meat Animal Research Center; Estados Unidos Fil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Larzabal, Mariano.Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Marques Da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Marques Da Silva, Wanderson. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Eberhardt, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Eberhardt, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Riviere, Nahuel Agustin. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Riviere, Nahuel Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Gally, David. University of Edinburgh. The Roslin Institute and R(D)SVS. Division of Immunity and Infection; Reino Unido Fil: Manning, Shannon D. Michigan State University. Department of Microbiology and Molecular Genetics; Estados Unidos Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-12-28T13:16:45Z 2021-12-28T13:16:45Z 2021-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11003 https://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0258753 1932-6203 https://doi.org/10.1371/journal.pone.0258753 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Plos PLoS ONE 16 (10) : e0258753 (2021) |
| spellingShingle | Ganado Bovino Escherichia coli Toxinas Enfermedades de los Animales Genética Molecular Argentina Cattle Toxins Animal Diseases Molecular Genetics Toxina Shiga Shiga Toxin Amadio, Ariel Bono, James L. Irazoqui, Jose Matias Larzabal, Mariano Marques Da Silva, Wanderson Eberhardt, María Florencia Riviere, Nahuel Agustin Gally, David Manning, Shannon D. Cataldi, Angel Adrian Genomic analysis of shiga toxin-containing Escherichia coli O157:H7 isolated from Argentinean cattle |
| title | Genomic analysis of shiga toxin-containing Escherichia coli O157:H7 isolated from Argentinean cattle |
| title_full | Genomic analysis of shiga toxin-containing Escherichia coli O157:H7 isolated from Argentinean cattle |
| title_fullStr | Genomic analysis of shiga toxin-containing Escherichia coli O157:H7 isolated from Argentinean cattle |
| title_full_unstemmed | Genomic analysis of shiga toxin-containing Escherichia coli O157:H7 isolated from Argentinean cattle |
| title_short | Genomic analysis of shiga toxin-containing Escherichia coli O157:H7 isolated from Argentinean cattle |
| title_sort | genomic analysis of shiga toxin containing escherichia coli o157 h7 isolated from argentinean cattle |
| topic | Ganado Bovino Escherichia coli Toxinas Enfermedades de los Animales Genética Molecular Argentina Cattle Toxins Animal Diseases Molecular Genetics Toxina Shiga Shiga Toxin |
| url | http://hdl.handle.net/20.500.12123/11003 https://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0258753 https://doi.org/10.1371/journal.pone.0258753 |
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