Diversity of the BoLA-DRB3 gene in cattle breeds from tropical and subtropical regions of Argentina
Bovine leukocyte antigens (BoLA) have been widely studied because of their primary function in the recognition of pathogens by the immune system. To date, however, the characterization of the BoLA-DRB3 gene in Latin American Zebu and mixed zebuine breeds is scarce. By a sequence-based typing method,...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Springer
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/11000 https://link.springer.com/article/10.1007%2Fs11250-021-03031-4 https://doi.org/10.1007/s11250-021-03031-4 |
| _version_ | 1855036491981389824 |
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| author | Valenzano, Magali Nicole Caffaro, María Eugenia Lia, Veronica Viviana Poli, Mario Andres Wilkowsky, Silvina Elizabeth |
| author_browse | Caffaro, María Eugenia Lia, Veronica Viviana Poli, Mario Andres Valenzano, Magali Nicole Wilkowsky, Silvina Elizabeth |
| author_facet | Valenzano, Magali Nicole Caffaro, María Eugenia Lia, Veronica Viviana Poli, Mario Andres Wilkowsky, Silvina Elizabeth |
| author_sort | Valenzano, Magali Nicole |
| collection | INTA Digital |
| description | Bovine leukocyte antigens (BoLA) have been widely studied because of their primary function in the recognition of pathogens by the immune system. To date, however, the characterization of the BoLA-DRB3 gene in Latin American Zebu and mixed zebuine breeds is scarce. By a sequence-based typing method, here we sequenced exon 2 of BoLA class II DRB3 gene in 264 animals from the five most commonly used breeds in northern Argentina (Creole, Brahman, Braford, Brangus, and Nellore).
The Bos taurus, Bos indicus, and mixed breeds analyzed here contained 61 previously reported alleles. Genetic diversity was high at both allelic and nucleotide sequence levels, particularly in the mixed breeds Braford and Brangus. In contrast to previous reports on DRB3 diversity, no evidence of balancing selection was found in our data. Differentiation among breeds was highly significant, as shown by FST (FST = 0.052, P < 0.001) and cluster analyses. In accordance with historical origin of the breeds, UPGMA trees and metric multidimensional scaling (MDS) analyses showed that Creole is distantly related to the other zebuine breeds. Among them, Brahman, Braford, and Brangus exhibited the closest affiliations. Despite the overall differentiation of the breeds, analysis of the peptide binding regions at the aminoacid level revealed that the key aminoacids involved in peptide recognition are greatly conserved suggesting little influence of domestication and breeding in functional MHC variability. In sum, this is the first report of BoLA-DRB3 diversity in pure and mixed Bos indicus cattle breeds from Argentina. Knowledge of BoLA-DRB3 variability in breeds adapted to tropical and subtropical environments contributes not only to the characterization of MHC diversity but also to the design of peptide-based vaccines. |
| format | info:ar-repo/semantics/artículo |
| id | INTA11000 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA110002021-12-28T12:27:52Z Diversity of the BoLA-DRB3 gene in cattle breeds from tropical and subtropical regions of Argentina Valenzano, Magali Nicole Caffaro, María Eugenia Lia, Veronica Viviana Poli, Mario Andres Wilkowsky, Silvina Elizabeth Enfermedades de los Animales Ganado Bovino Razas (animales) Antígenos Diversidad Genética (como Recurso) Animal Diseases Cattle Breeds (animals) Antigens Genetic Diversity (as Resource) Bovine Leucocyte Antigen Tuberculosis Bovina BoLA-DRB3 Bovine leukocyte antigens (BoLA) have been widely studied because of their primary function in the recognition of pathogens by the immune system. To date, however, the characterization of the BoLA-DRB3 gene in Latin American Zebu and mixed zebuine breeds is scarce. By a sequence-based typing method, here we sequenced exon 2 of BoLA class II DRB3 gene in 264 animals from the five most commonly used breeds in northern Argentina (Creole, Brahman, Braford, Brangus, and Nellore). The Bos taurus, Bos indicus, and mixed breeds analyzed here contained 61 previously reported alleles. Genetic diversity was high at both allelic and nucleotide sequence levels, particularly in the mixed breeds Braford and Brangus. In contrast to previous reports on DRB3 diversity, no evidence of balancing selection was found in our data. Differentiation among breeds was highly significant, as shown by FST (FST = 0.052, P < 0.001) and cluster analyses. In accordance with historical origin of the breeds, UPGMA trees and metric multidimensional scaling (MDS) analyses showed that Creole is distantly related to the other zebuine breeds. Among them, Brahman, Braford, and Brangus exhibited the closest affiliations. Despite the overall differentiation of the breeds, analysis of the peptide binding regions at the aminoacid level revealed that the key aminoacids involved in peptide recognition are greatly conserved suggesting little influence of domestication and breeding in functional MHC variability. In sum, this is the first report of BoLA-DRB3 diversity in pure and mixed Bos indicus cattle breeds from Argentina. Knowledge of BoLA-DRB3 variability in breeds adapted to tropical and subtropical environments contributes not only to the characterization of MHC diversity but also to the design of peptide-based vaccines. Instituto de Biotecnología Fil: Valenzano, Magalí Nicole. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Valenzano, Magalí Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Caffaro, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Lia, Veronica Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Wilkowsky, Silvina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-12-28T12:25:40Z 2021-12-28T12:25:40Z 2021-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11000 https://link.springer.com/article/10.1007%2Fs11250-021-03031-4 0049-4747 1573-7438 https://doi.org/10.1007/s11250-021-03031-4 eng info:eu-repograntAgreement/INTA/2019-PE-E6-I114-001/2019-PE-E6-I114-001/AR./Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada. info:eu-repograntAgreement/INTA/2019-PE-E6-I145-001/2019-PE-E6-I145-001/AR./Mejora genética objetiva para aumentar la eficiencia de los sistemas de producción animal. info:eu-repo/semantics/restrictedAccess application/pdf Springer Tropical Animal Health and Production 54 : Article number: 23 (2022) |
| spellingShingle | Enfermedades de los Animales Ganado Bovino Razas (animales) Antígenos Diversidad Genética (como Recurso) Animal Diseases Cattle Breeds (animals) Antigens Genetic Diversity (as Resource) Bovine Leucocyte Antigen Tuberculosis Bovina BoLA-DRB3 Valenzano, Magali Nicole Caffaro, María Eugenia Lia, Veronica Viviana Poli, Mario Andres Wilkowsky, Silvina Elizabeth Diversity of the BoLA-DRB3 gene in cattle breeds from tropical and subtropical regions of Argentina |
| title | Diversity of the BoLA-DRB3 gene in cattle breeds from tropical and subtropical regions of Argentina |
| title_full | Diversity of the BoLA-DRB3 gene in cattle breeds from tropical and subtropical regions of Argentina |
| title_fullStr | Diversity of the BoLA-DRB3 gene in cattle breeds from tropical and subtropical regions of Argentina |
| title_full_unstemmed | Diversity of the BoLA-DRB3 gene in cattle breeds from tropical and subtropical regions of Argentina |
| title_short | Diversity of the BoLA-DRB3 gene in cattle breeds from tropical and subtropical regions of Argentina |
| title_sort | diversity of the bola drb3 gene in cattle breeds from tropical and subtropical regions of argentina |
| topic | Enfermedades de los Animales Ganado Bovino Razas (animales) Antígenos Diversidad Genética (como Recurso) Animal Diseases Cattle Breeds (animals) Antigens Genetic Diversity (as Resource) Bovine Leucocyte Antigen Tuberculosis Bovina BoLA-DRB3 |
| url | http://hdl.handle.net/20.500.12123/11000 https://link.springer.com/article/10.1007%2Fs11250-021-03031-4 https://doi.org/10.1007/s11250-021-03031-4 |
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