Localization and survival of Azospirillum brasilense Az39 in soybean leaves
In recent years, foliar inoculation has gained acceptance among the available methods to deliver plant beneficial micro-organisms to crops under field conditions. Colonization efficiency by such micro-organisms largely depends on their ability to survive when applied on the leaves. In this work, we...
| Main Authors: | , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Wiley
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/10996 https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/lam.13444 https://doi.org/10.1111/lam.13444 |
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| author | Puente, Mariana Laura Maroniche, Guillermo Andrés Panepucci, Marina Gabriela Sabio Y Garcia, Julia Veronica García, Julia Elena Criado, María Victoria Molina, Romina Micaela Cassán, Fabricio Dario |
| author_browse | Cassán, Fabricio Dario Criado, María Victoria García, Julia Elena Maroniche, Guillermo Andrés Molina, Romina Micaela Panepucci, Marina Gabriela Puente, Mariana Laura Sabio Y Garcia, Julia Veronica |
| author_facet | Puente, Mariana Laura Maroniche, Guillermo Andrés Panepucci, Marina Gabriela Sabio Y Garcia, Julia Veronica García, Julia Elena Criado, María Victoria Molina, Romina Micaela Cassán, Fabricio Dario |
| author_sort | Puente, Mariana Laura |
| collection | INTA Digital |
| description | In recent years, foliar inoculation has gained acceptance among the available methods to deliver plant beneficial micro-organisms to crops under field conditions. Colonization efficiency by such micro-organisms largely depends on their ability to survive when applied on the leaves. In this work, we evaluated the survival and localization of Azospirillum brasilense Az39 (Az39) in excised soybean leaves. Scanning electron microscopy and confocal laser scanning microscopy of a red fluorescent-transformed variant of Az39 were used to determine bacterial localization, while the most probable number and plate count methods were applied for bacterial quantification. Microscopic observations indicated a decrease in the number of Az39 cells on the leaf surface at 24 h after treatment, whereas midribs and cell–cell junctions of the inner leaf epidermis became highly populated zones. The presence of Az39 inside xylem vessels was corroborated at 6 h after bacterization. Az39 population did not significantly decrease throughout 24 h. We could visualize Az39 cells on the surface and in internal tissues of soybean leaves and recover them through culture methodologies. These results evidence the survival capacity of Az39 on and inside leaves and suggest a previously unnoticed endophytic potential for this well-known plant growth-promoting rhizobacteria strain. |
| format | Artículo |
| id | INTA10996 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA109962021-12-27T17:05:50Z Localization and survival of Azospirillum brasilense Az39 in soybean leaves Puente, Mariana Laura Maroniche, Guillermo Andrés Panepucci, Marina Gabriela Sabio Y Garcia, Julia Veronica García, Julia Elena Criado, María Victoria Molina, Romina Micaela Cassán, Fabricio Dario Azospirillum brasilense Inoculation Soybeans Soja Inoculación PGPR In recent years, foliar inoculation has gained acceptance among the available methods to deliver plant beneficial micro-organisms to crops under field conditions. Colonization efficiency by such micro-organisms largely depends on their ability to survive when applied on the leaves. In this work, we evaluated the survival and localization of Azospirillum brasilense Az39 (Az39) in excised soybean leaves. Scanning electron microscopy and confocal laser scanning microscopy of a red fluorescent-transformed variant of Az39 were used to determine bacterial localization, while the most probable number and plate count methods were applied for bacterial quantification. Microscopic observations indicated a decrease in the number of Az39 cells on the leaf surface at 24 h after treatment, whereas midribs and cell–cell junctions of the inner leaf epidermis became highly populated zones. The presence of Az39 inside xylem vessels was corroborated at 6 h after bacterization. Az39 population did not significantly decrease throughout 24 h. We could visualize Az39 cells on the surface and in internal tissues of soybean leaves and recover them through culture methodologies. These results evidence the survival capacity of Az39 on and inside leaves and suggest a previously unnoticed endophytic potential for this well-known plant growth-promoting rhizobacteria strain. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Laboratorio de Bacterias Promotoras del Crecimiento Vegetal; Argentina Fil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Panepucci, Marina Gabriela. Universidad de Buenos Aires. Facultad de Agronomía; Argentina Fil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; Argentina Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Criado, Maria Victoria. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA)-CONICET; Argentina Fil: Molina, Romina Micaela. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. Laboratorio de Fisiología Vegetal e Interacción Planta-Microorganismo. INIAB-CONICET; Argentina Fil: Cassán, Fabricio Darío. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. Laboratorio de Fisiología Vegetal e Interacción Planta-Microorganismo. INIAB-CONICET; Argentina 2021-12-27T16:49:22Z 2021-12-27T16:49:22Z 2021-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10996 https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/lam.13444 0266-8254 https://doi.org/10.1111/lam.13444 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Letters in Applied Microbiology 72 (5) : 626-633 (May 2021) |
| spellingShingle | Azospirillum brasilense Inoculation Soybeans Soja Inoculación PGPR Puente, Mariana Laura Maroniche, Guillermo Andrés Panepucci, Marina Gabriela Sabio Y Garcia, Julia Veronica García, Julia Elena Criado, María Victoria Molina, Romina Micaela Cassán, Fabricio Dario Localization and survival of Azospirillum brasilense Az39 in soybean leaves |
| title | Localization and survival of Azospirillum brasilense Az39 in soybean leaves |
| title_full | Localization and survival of Azospirillum brasilense Az39 in soybean leaves |
| title_fullStr | Localization and survival of Azospirillum brasilense Az39 in soybean leaves |
| title_full_unstemmed | Localization and survival of Azospirillum brasilense Az39 in soybean leaves |
| title_short | Localization and survival of Azospirillum brasilense Az39 in soybean leaves |
| title_sort | localization and survival of azospirillum brasilense az39 in soybean leaves |
| topic | Azospirillum brasilense Inoculation Soybeans Soja Inoculación PGPR |
| url | http://hdl.handle.net/20.500.12123/10996 https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/lam.13444 https://doi.org/10.1111/lam.13444 |
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