Comparative Pathogenesis of Generalist AcMNPV and Specific RanuNPV in Larvae of Rachiplusia nu (Lepidoptera: Noctuidae) Following Single and Mixed Inoculations

The South American soybean pest, Rachiplusia nu (Guenée), is naturally infected by Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Rachiplusia nu nucleopolyhedrovirus (RanuNPV). We compared their pathogenicity to fourth-instar R. nu larvae, by evaluating time to death and virus spr...

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Main Authors: Decker Franco, Cecilia, Taibo, Catalina Beatriz, Di Rienzo, Julio A., Alfonso, Victoria, Arneodo Larochette, Joel Demian
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Oxford University Press 2021
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/10995
https://academic.oup.com/jee/article/114/2/1009/6144816
https://doi.org/10.1093/jee/toab010
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author Decker Franco, Cecilia
Taibo, Catalina Beatriz
Di Rienzo, Julio A.
Alfonso, Victoria
Arneodo Larochette, Joel Demian
author_browse Alfonso, Victoria
Arneodo Larochette, Joel Demian
Decker Franco, Cecilia
Di Rienzo, Julio A.
Taibo, Catalina Beatriz
author_facet Decker Franco, Cecilia
Taibo, Catalina Beatriz
Di Rienzo, Julio A.
Alfonso, Victoria
Arneodo Larochette, Joel Demian
author_sort Decker Franco, Cecilia
collection INTA Digital
description The South American soybean pest, Rachiplusia nu (Guenée), is naturally infected by Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Rachiplusia nu nucleopolyhedrovirus (RanuNPV). We compared their pathogenicity to fourth-instar R. nu larvae, by evaluating time to death and virus spread throughout the tissues in single and mixed infections. Bioassays showed that generalist AcMNPV had a faster speed of kill than specific RanuNPV, while the mixed-virus treatment did not statistically differ from AcMNPV alone. Histopathology evidenced similar tissue tropism for both viruses, but co-inoculation resulted in mostly AcMNPV-infected cells. In sequential inoculations, however, the first virus administered predominated over the second one. Implications on baculovirus interactions and biocontrol potential are discussed.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
publishDate 2021
publishDateRange 2021
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publisher Oxford University Press
publisherStr Oxford University Press
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spelling INTA109952022-08-12T16:23:42Z Comparative Pathogenesis of Generalist AcMNPV and Specific RanuNPV in Larvae of Rachiplusia nu (Lepidoptera: Noctuidae) Following Single and Mixed Inoculations Decker Franco, Cecilia Taibo, Catalina Beatriz Di Rienzo, Julio A. Alfonso, Victoria Arneodo Larochette, Joel Demian Rachiplusia Pathogenesis Patogénesis Viral Insecticide Rachiplusia nu Nucleopolyhedrovirus Mixed Infections The South American soybean pest, Rachiplusia nu (Guenée), is naturally infected by Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Rachiplusia nu nucleopolyhedrovirus (RanuNPV). We compared their pathogenicity to fourth-instar R. nu larvae, by evaluating time to death and virus spread throughout the tissues in single and mixed infections. Bioassays showed that generalist AcMNPV had a faster speed of kill than specific RanuNPV, while the mixed-virus treatment did not statistically differ from AcMNPV alone. Histopathology evidenced similar tissue tropism for both viruses, but co-inoculation resulted in mostly AcMNPV-infected cells. In sequential inoculations, however, the first virus administered predominated over the second one. Implications on baculovirus interactions and biocontrol potential are discussed. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Taibo, Catalina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; Argentina Fil: Di Rienzo, Julio A. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Estadística y Biometría; Argentina Fil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina Fil: Arneodo, Joel D. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Arneodo, Joel D. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina 2021-12-27T16:24:34Z 2021-12-27T16:24:34Z 2021-02-19 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10995 https://academic.oup.com/jee/article/114/2/1009/6144816 1938-291X https://doi.org/10.1093/jee/toab010 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Oxford University Press Journal of Economic Entomology 114 (2) : 1009-1014 (April 2021)
spellingShingle Rachiplusia
Pathogenesis
Patogénesis
Viral Insecticide
Rachiplusia nu
Nucleopolyhedrovirus
Mixed Infections
Decker Franco, Cecilia
Taibo, Catalina Beatriz
Di Rienzo, Julio A.
Alfonso, Victoria
Arneodo Larochette, Joel Demian
Comparative Pathogenesis of Generalist AcMNPV and Specific RanuNPV in Larvae of Rachiplusia nu (Lepidoptera: Noctuidae) Following Single and Mixed Inoculations
title Comparative Pathogenesis of Generalist AcMNPV and Specific RanuNPV in Larvae of Rachiplusia nu (Lepidoptera: Noctuidae) Following Single and Mixed Inoculations
title_full Comparative Pathogenesis of Generalist AcMNPV and Specific RanuNPV in Larvae of Rachiplusia nu (Lepidoptera: Noctuidae) Following Single and Mixed Inoculations
title_fullStr Comparative Pathogenesis of Generalist AcMNPV and Specific RanuNPV in Larvae of Rachiplusia nu (Lepidoptera: Noctuidae) Following Single and Mixed Inoculations
title_full_unstemmed Comparative Pathogenesis of Generalist AcMNPV and Specific RanuNPV in Larvae of Rachiplusia nu (Lepidoptera: Noctuidae) Following Single and Mixed Inoculations
title_short Comparative Pathogenesis of Generalist AcMNPV and Specific RanuNPV in Larvae of Rachiplusia nu (Lepidoptera: Noctuidae) Following Single and Mixed Inoculations
title_sort comparative pathogenesis of generalist acmnpv and specific ranunpv in larvae of rachiplusia nu lepidoptera noctuidae following single and mixed inoculations
topic Rachiplusia
Pathogenesis
Patogénesis
Viral Insecticide
Rachiplusia nu
Nucleopolyhedrovirus
Mixed Infections
url http://hdl.handle.net/20.500.12123/10995
https://academic.oup.com/jee/article/114/2/1009/6144816
https://doi.org/10.1093/jee/toab010
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