Identification and characterization of soluble binding proteins associated with host foraging in the parasitoid wasp Diachasmimorpha longicaudata
The communication and reproduction of insects are driven by chemical sensing. During this process, chemical compounds are transported across the sensillum lymph to the sensory neurons assisted by different types of soluble binding proteins: odorant-binding proteins (OBPs); chemosensory proteins (CSP...
| Main Authors: | , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Plos
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/10991 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0252765 https://doi.org/10.1371/journal.pone.0252765 |
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| author | Wulff, Juan Pedro Segura, Diego Fernando Devescovi, Francisco Muntaabski, Irina Milla, Fabian Horacio Scannapieco, Alejandra Carla Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz |
| author_browse | Cladera, Jorge Luis Devescovi, Francisco Lanzavecchia, Silvia Beatriz Milla, Fabian Horacio Muntaabski, Irina Scannapieco, Alejandra Carla Segura, Diego Fernando Wulff, Juan Pedro |
| author_facet | Wulff, Juan Pedro Segura, Diego Fernando Devescovi, Francisco Muntaabski, Irina Milla, Fabian Horacio Scannapieco, Alejandra Carla Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz |
| author_sort | Wulff, Juan Pedro |
| collection | INTA Digital |
| description | The communication and reproduction of insects are driven by chemical sensing. During this process, chemical compounds are transported across the sensillum lymph to the sensory neurons assisted by different types of soluble binding proteins: odorant-binding proteins (OBPs); chemosensory proteins (CSPs); some members of ML-family proteins (MD-2 (myeloid differentiation factor-2)-related Lipid-recognition), also known as NPC2-like proteins. Potential transcripts involved in chemosensing were identified by an in silico analysis of whole-body female and male transcriptomes of the parasitic wasp Diachasmimorpha longicaudata. This analysis facilitated the characterization of fourteen OBPs (all belonging to the Classic type), seven CSPs (and two possible isoforms), and four NPC2-like proteins. A differential expression analysis by qPCR showed that eleven of these proteins (CSPs 2 and 8, OBPs 2, 3, 4, 5, 6, 9, 10, and 11, and NPC2b) were over-expressed in female antenna and two (CSP 1 and OBP 12) in the body without antennae. Foraging behavior trials (linked to RNA interference) suggest that OBPs 9, 10, and 11 are potentially involved in the female orientation to chemical cues associated with the host. OBP 12 seems to be related to physiological processes of female longevity regulation. In addition, transcriptional silencing of CSP 3 showed that this protein is potentially associated with the regulation of foraging behavior. This study supports the hypothesis that soluble binding proteins are potentially linked to fundamental physiological processes and behaviors in D. longicaudata. The results obtained here contribute useful information to increase the parasitoid performance as a biological control agent of fruit fly pest species. |
| format | info:ar-repo/semantics/artículo |
| id | INTA10991 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Plos |
| publisherStr | Plos |
| record_format | dspace |
| spelling | INTA109912021-12-27T13:23:36Z Identification and characterization of soluble binding proteins associated with host foraging in the parasitoid wasp Diachasmimorpha longicaudata Wulff, Juan Pedro Segura, Diego Fernando Devescovi, Francisco Muntaabski, Irina Milla, Fabian Horacio Scannapieco, Alejandra Carla Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz Biosteres longicaudatus Parasitoides Control de Plagas Proteínas Parasitoids Pest Control Proteins Diachasmimorpha longicaudata The communication and reproduction of insects are driven by chemical sensing. During this process, chemical compounds are transported across the sensillum lymph to the sensory neurons assisted by different types of soluble binding proteins: odorant-binding proteins (OBPs); chemosensory proteins (CSPs); some members of ML-family proteins (MD-2 (myeloid differentiation factor-2)-related Lipid-recognition), also known as NPC2-like proteins. Potential transcripts involved in chemosensing were identified by an in silico analysis of whole-body female and male transcriptomes of the parasitic wasp Diachasmimorpha longicaudata. This analysis facilitated the characterization of fourteen OBPs (all belonging to the Classic type), seven CSPs (and two possible isoforms), and four NPC2-like proteins. A differential expression analysis by qPCR showed that eleven of these proteins (CSPs 2 and 8, OBPs 2, 3, 4, 5, 6, 9, 10, and 11, and NPC2b) were over-expressed in female antenna and two (CSP 1 and OBP 12) in the body without antennae. Foraging behavior trials (linked to RNA interference) suggest that OBPs 9, 10, and 11 are potentially involved in the female orientation to chemical cues associated with the host. OBP 12 seems to be related to physiological processes of female longevity regulation. In addition, transcriptional silencing of CSP 3 showed that this protein is potentially associated with the regulation of foraging behavior. This study supports the hypothesis that soluble binding proteins are potentially linked to fundamental physiological processes and behaviors in D. longicaudata. The results obtained here contribute useful information to increase the parasitoid performance as a biological control agent of fruit fly pest species. Instituto de Genética Fil: Wulff, Juan Pedro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina. Fil: Wulff, Juan Pedro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina. Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Devescovi, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina. Fil: Devescovi, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina. Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Milla, Fabian Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Fil: Milla, Fabian Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina. Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina. Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina. Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. 2021-12-27T13:21:16Z 2021-12-27T13:21:16Z 2021-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10991 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0252765 1932-6203 https://doi.org/10.1371/journal.pone.0252765 eng info:eu-repograntAgreement/INTA/2019-PD-E4-I079-001/2019-PD-E4-I079-001/AR./Genética, genómica y ecología de insectos de importancia agronómica como insumo para el desarrollo de estrategias sustentables de control plagas info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001/2019-PD-E6-I116-001/AR./Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Plos PLoS ONE 16 (6) : e0252765. (June 2021) |
| spellingShingle | Biosteres longicaudatus Parasitoides Control de Plagas Proteínas Parasitoids Pest Control Proteins Diachasmimorpha longicaudata Wulff, Juan Pedro Segura, Diego Fernando Devescovi, Francisco Muntaabski, Irina Milla, Fabian Horacio Scannapieco, Alejandra Carla Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz Identification and characterization of soluble binding proteins associated with host foraging in the parasitoid wasp Diachasmimorpha longicaudata |
| title | Identification and characterization of soluble binding proteins associated with host foraging in the parasitoid wasp Diachasmimorpha longicaudata |
| title_full | Identification and characterization of soluble binding proteins associated with host foraging in the parasitoid wasp Diachasmimorpha longicaudata |
| title_fullStr | Identification and characterization of soluble binding proteins associated with host foraging in the parasitoid wasp Diachasmimorpha longicaudata |
| title_full_unstemmed | Identification and characterization of soluble binding proteins associated with host foraging in the parasitoid wasp Diachasmimorpha longicaudata |
| title_short | Identification and characterization of soluble binding proteins associated with host foraging in the parasitoid wasp Diachasmimorpha longicaudata |
| title_sort | identification and characterization of soluble binding proteins associated with host foraging in the parasitoid wasp diachasmimorpha longicaudata |
| topic | Biosteres longicaudatus Parasitoides Control de Plagas Proteínas Parasitoids Pest Control Proteins Diachasmimorpha longicaudata |
| url | http://hdl.handle.net/20.500.12123/10991 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0252765 https://doi.org/10.1371/journal.pone.0252765 |
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