Physiological characterization of four model Lotus diploid genotypes: L. japonicus (MG20 and Gifu), L. filicaulis, and L. burttii under salt stress
The genus Lotus comprises a heterogeneous group of annual and perennial species. Lotus japonicus (with MG20 and Gifu ecotypes) has been adopted as one of the model legumes in genetic and genomic studies. Other Lotus species, such us Lotus burttii and Lotus filicaulis, have also been used in genetic...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/10979 https://www.sciencedirect.com/science/article/pii/S0168945209002581 https://doi.org/10.1016/j.plantsci.2009.09.010 |
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| author | Melchiorre, Mariana Quero, Gastón E. Parola, Rodrigo Racca, Roberto Walter Trippi, Victorio Lascano, Hernan Ramiro |
| author_browse | Lascano, Hernan Ramiro Melchiorre, Mariana Parola, Rodrigo Quero, Gastón E. Racca, Roberto Walter Trippi, Victorio |
| author_facet | Melchiorre, Mariana Quero, Gastón E. Parola, Rodrigo Racca, Roberto Walter Trippi, Victorio Lascano, Hernan Ramiro |
| author_sort | Melchiorre, Mariana |
| collection | INTA Digital |
| description | The genus Lotus comprises a heterogeneous group of annual and perennial species. Lotus japonicus (with MG20 and Gifu ecotypes) has been adopted as one of the model legumes in genetic and genomic studies. Other Lotus species, such us Lotus burttii and Lotus filicaulis, have also been used in genetic and genomic studies because of their capacity to produce fertile progenies in crosses with L. japonicus. In the present work, physiological responses to salt stress in four Lotus genotypes were evaluated on the basis of growth and associated parameters, such as photosynthesis, ions, relative water content, oxidative damage and antioxidant system responses, using two NaCl levels applied by acclimation for up to 28 and 60 d. Growth responses varied with plant developmental stage in the four Lotus genotypes. L. japonicus MG20 was found to be a salt-tolerant genotype, mainly when exposed to salt stress at the young plant stage. The capacity of Lj MG20 to sustain growth under salt stress was correlated with enhancement of Superoxide dismutase and Glutathione reductase activities, as well as with increases in total and reduced glutathione content and lower Na+ accumulation in leaves. These results suggest that enhancement of antioxidant responses in Lj MG20 contributed to improve salt stress tolerance at early stages. On the other hand, after long-term high NaCl stress treatment, L. filicaulis exhibited lower biomass reduction, lower oxidative damage and Na+ accumulation in leaves than the control treatment; hence, this genotype was considered salt-tolerant. These apparently ambiguous results remark that salt tolerance, as a development-related process, was differentially expressed among the Lotus genotypes and depended on stress duration and plant phenological stage. |
| format | Artículo |
| id | INTA10979 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA109792021-12-23T11:15:24Z Physiological characterization of four model Lotus diploid genotypes: L. japonicus (MG20 and Gifu), L. filicaulis, and L. burttii under salt stress Melchiorre, Mariana Quero, Gastón E. Parola, Rodrigo Racca, Roberto Walter Trippi, Victorio Lascano, Hernan Ramiro Ions Salinity Osmotic Stress Lotus Estrés Osmótico Iones Salinidad Antioxidant System Phenotyping Estrés Salino The genus Lotus comprises a heterogeneous group of annual and perennial species. Lotus japonicus (with MG20 and Gifu ecotypes) has been adopted as one of the model legumes in genetic and genomic studies. Other Lotus species, such us Lotus burttii and Lotus filicaulis, have also been used in genetic and genomic studies because of their capacity to produce fertile progenies in crosses with L. japonicus. In the present work, physiological responses to salt stress in four Lotus genotypes were evaluated on the basis of growth and associated parameters, such as photosynthesis, ions, relative water content, oxidative damage and antioxidant system responses, using two NaCl levels applied by acclimation for up to 28 and 60 d. Growth responses varied with plant developmental stage in the four Lotus genotypes. L. japonicus MG20 was found to be a salt-tolerant genotype, mainly when exposed to salt stress at the young plant stage. The capacity of Lj MG20 to sustain growth under salt stress was correlated with enhancement of Superoxide dismutase and Glutathione reductase activities, as well as with increases in total and reduced glutathione content and lower Na+ accumulation in leaves. These results suggest that enhancement of antioxidant responses in Lj MG20 contributed to improve salt stress tolerance at early stages. On the other hand, after long-term high NaCl stress treatment, L. filicaulis exhibited lower biomass reduction, lower oxidative damage and Na+ accumulation in leaves than the control treatment; hence, this genotype was considered salt-tolerant. These apparently ambiguous results remark that salt tolerance, as a development-related process, was differentially expressed among the Lotus genotypes and depended on stress duration and plant phenological stage. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina Fil: Quero, Gastón E. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina Fil: Quero, Gastón E. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Parola, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina Fil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina Fil: Trippi, Victorio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Trippi, Victorio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina 2021-12-23T11:08:05Z 2021-12-23T11:08:05Z 2009-09-25 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10979 https://www.sciencedirect.com/science/article/pii/S0168945209002581 0168-9452 https://doi.org/10.1016/j.plantsci.2009.09.010 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Plant Science 177 (6) : 618-628 (December 2009) |
| spellingShingle | Ions Salinity Osmotic Stress Lotus Estrés Osmótico Iones Salinidad Antioxidant System Phenotyping Estrés Salino Melchiorre, Mariana Quero, Gastón E. Parola, Rodrigo Racca, Roberto Walter Trippi, Victorio Lascano, Hernan Ramiro Physiological characterization of four model Lotus diploid genotypes: L. japonicus (MG20 and Gifu), L. filicaulis, and L. burttii under salt stress |
| title | Physiological characterization of four model Lotus diploid genotypes: L. japonicus (MG20 and Gifu), L. filicaulis, and L. burttii under salt stress |
| title_full | Physiological characterization of four model Lotus diploid genotypes: L. japonicus (MG20 and Gifu), L. filicaulis, and L. burttii under salt stress |
| title_fullStr | Physiological characterization of four model Lotus diploid genotypes: L. japonicus (MG20 and Gifu), L. filicaulis, and L. burttii under salt stress |
| title_full_unstemmed | Physiological characterization of four model Lotus diploid genotypes: L. japonicus (MG20 and Gifu), L. filicaulis, and L. burttii under salt stress |
| title_short | Physiological characterization of four model Lotus diploid genotypes: L. japonicus (MG20 and Gifu), L. filicaulis, and L. burttii under salt stress |
| title_sort | physiological characterization of four model lotus diploid genotypes l japonicus mg20 and gifu l filicaulis and l burttii under salt stress |
| topic | Ions Salinity Osmotic Stress Lotus Estrés Osmótico Iones Salinidad Antioxidant System Phenotyping Estrés Salino |
| url | http://hdl.handle.net/20.500.12123/10979 https://www.sciencedirect.com/science/article/pii/S0168945209002581 https://doi.org/10.1016/j.plantsci.2009.09.010 |
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