Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state
In previous works, we have established a correlation between antioxidant system response and tolerance to drought, osmotic stress and photooxidative stress of different wheat cultivars with contrasting drought tolerance. In the present work, a protocol to obtain and transform wheat protoplasts was e...
| Main Authors: | , , , , |
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| Format: | Artículo |
| Language: | Alemán |
| Published: |
Springer
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/10978 https://link.springer.com/article/10.1007/s10725-008-9322-3 https://doi.org/10.1007/s10725-008-9322-3 |
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| author | Melchiorre, Mariana Robert, German Trippi, Victorio Racca, Roberto Walter Lascano, Hernan Ramiro |
| author_browse | Lascano, Hernan Ramiro Melchiorre, Mariana Racca, Roberto Walter Robert, German Trippi, Victorio |
| author_facet | Melchiorre, Mariana Robert, German Trippi, Victorio Racca, Roberto Walter Lascano, Hernan Ramiro |
| author_sort | Melchiorre, Mariana |
| collection | INTA Digital |
| description | In previous works, we have established a correlation between antioxidant system response and tolerance to drought, osmotic stress and photooxidative stress of different wheat cultivars with contrasting drought tolerance. In the present work, a protocol to obtain and transform wheat protoplasts was established. Transgenic protoplasts with Manganese Superoxide Dismutase (Mn-SOD) (E.C.: 1.15.1.1) and Glutathione Reductase (GR) (E.C.: 1.6.4.2) overexpression in chloroplasts were obtained, and their responses to photooxidative stress were characterized. Protoplasts with Mn-SOD or GR overexpression, showed different responses and tolerance to photooxidative stress. Protoplasts with Mn-SOD overexpression showed lower levels of oxidative damage, higher level of endogenous hydrogen peroxide and a great induction of total SOD and GR activities during photooxidative treatments. In protoplasts with GR overexpression the oxidative damage provoked by the photooxidative treatment was similar to control protoplasts, the GSH content and GSH/GSH + GSSG ratio were higher than control and Mn-SOD transformed protoplast, and total SOD and GR activities were not induced. Our results suggest that the differential responses and tolerance to photooxidative stress given by Mn-SOD or GR overexpression, also depend on the effects of these enzyme activities over the cellular redox state balance, which modulate the responses to photooxidative stress. |
| format | Artículo |
| id | INTA10978 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Alemán |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA109782021-12-23T11:05:22Z Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state Melchiorre, Mariana Robert, German Trippi, Victorio Racca, Roberto Walter Lascano, Hernan Ramiro Glutathione Reductase Reactive Oxygen Species Superoxide Dismutase Glutatión Reductasa Superóxido Dismutasa Especies de Oxígeno Reactivo Photooxidative Stress Wheat Protoplasts Estrés Fotooxidativo In previous works, we have established a correlation between antioxidant system response and tolerance to drought, osmotic stress and photooxidative stress of different wheat cultivars with contrasting drought tolerance. In the present work, a protocol to obtain and transform wheat protoplasts was established. Transgenic protoplasts with Manganese Superoxide Dismutase (Mn-SOD) (E.C.: 1.15.1.1) and Glutathione Reductase (GR) (E.C.: 1.6.4.2) overexpression in chloroplasts were obtained, and their responses to photooxidative stress were characterized. Protoplasts with Mn-SOD or GR overexpression, showed different responses and tolerance to photooxidative stress. Protoplasts with Mn-SOD overexpression showed lower levels of oxidative damage, higher level of endogenous hydrogen peroxide and a great induction of total SOD and GR activities during photooxidative treatments. In protoplasts with GR overexpression the oxidative damage provoked by the photooxidative treatment was similar to control protoplasts, the GSH content and GSH/GSH + GSSG ratio were higher than control and Mn-SOD transformed protoplast, and total SOD and GR activities were not induced. Our results suggest that the differential responses and tolerance to photooxidative stress given by Mn-SOD or GR overexpression, also depend on the effects of these enzyme activities over the cellular redox state balance, which modulate the responses to photooxidative stress. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina Fil: Trippi, Victorio. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Trippi, Victorio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina Fil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina Argentina 2021-12-23T10:54:41Z 2021-12-23T10:54:41Z 2008-08-26 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10978 https://link.springer.com/article/10.1007/s10725-008-9322-3 0167-6903 1573-5087 (online) https://doi.org/10.1007/s10725-008-9322-3 deu info:eu-repo/semantics/restrictedAccess application/pdf Springer Plant Growth Regulation 57 : 57-68 (2009) |
| spellingShingle | Glutathione Reductase Reactive Oxygen Species Superoxide Dismutase Glutatión Reductasa Superóxido Dismutasa Especies de Oxígeno Reactivo Photooxidative Stress Wheat Protoplasts Estrés Fotooxidativo Melchiorre, Mariana Robert, German Trippi, Victorio Racca, Roberto Walter Lascano, Hernan Ramiro Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state |
| title | Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state |
| title_full | Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state |
| title_fullStr | Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state |
| title_full_unstemmed | Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state |
| title_short | Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state |
| title_sort | superoxide dismutase and glutathione reductase overexpression in wheat protoplast photooxidative stress tolerance and changes in cellular redox state |
| topic | Glutathione Reductase Reactive Oxygen Species Superoxide Dismutase Glutatión Reductasa Superóxido Dismutasa Especies de Oxígeno Reactivo Photooxidative Stress Wheat Protoplasts Estrés Fotooxidativo |
| url | http://hdl.handle.net/20.500.12123/10978 https://link.springer.com/article/10.1007/s10725-008-9322-3 https://doi.org/10.1007/s10725-008-9322-3 |
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