Plastome mutations and recombination events in barley chloroplast mutator seedlings
The barley chloroplast mutator (cpm) is an allele of a nuclear gene that when homozygous induces several types of cytoplasmically inherited chlorophyll deficiencies. In this work, a plastome Targeting Induced Local Lesions in Genomes (TILLING) strategy based on mismatch digestion was used on familie...
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| Format: | Artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1097 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4885237/ https://doi.org/10.1093/jhered/esw003 |
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| author | Landau, Alejandra Mabel Lencina, Franco Pacheco, Maria Gabriela Prina, Alberto Raul |
| author_browse | Landau, Alejandra Mabel Lencina, Franco Pacheco, Maria Gabriela Prina, Alberto Raul |
| author_facet | Landau, Alejandra Mabel Lencina, Franco Pacheco, Maria Gabriela Prina, Alberto Raul |
| author_sort | Landau, Alejandra Mabel |
| collection | INTA Digital |
| description | The barley chloroplast mutator (cpm) is an allele of a nuclear gene that when homozygous induces several types of cytoplasmically inherited chlorophyll deficiencies. In this work, a plastome Targeting Induced Local Lesions in Genomes (TILLING) strategy based on mismatch digestion was used on families that carried the cpm genotype through many generations. Extensive scanning of
33 plastome genes and a few intergenic regions was conducted. Numerous polymorphisms were detected on both genic and intergenic regions. The detected polymorphisms can be accounted for by at least 61 independent mutational events. The vast majority of the polymorphisms originated in substitutions and small indels (insertions/deletions) in microsatellites. The rpl23 and the rps16 genes were the most polymorphic. Interestingly, the variation observed in the rpl23 gene consisted of several combinations of 5 different one nucleotide polymorphisms. Besides, 4 large indels that have direct repeats at both ends were also observed, which appear to be originated from recombinational events. The cpm mutation spectrum suggests that the CPM gene product is probably involved in plastome mismatch repair. The numerous subtle molecular changes that were localized in a wide range of plastome sites show the cpm as a valuable source of plastome variability for plant research and/or plant breeding. Moreover, the cpm mutant appears to be an interesting experimental material for investigating the mechanisms responsible for maintaining the stability of plant organelle DNA. |
| format | Artículo |
| id | INTA1097 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA10972018-01-02T13:20:26Z Plastome mutations and recombination events in barley chloroplast mutator seedlings Landau, Alejandra Mabel Lencina, Franco Pacheco, Maria Gabriela Prina, Alberto Raul Genética Cebada Polimorfismo Cloroplasto Genetics Barley Polymorphism Chloroplasts The barley chloroplast mutator (cpm) is an allele of a nuclear gene that when homozygous induces several types of cytoplasmically inherited chlorophyll deficiencies. In this work, a plastome Targeting Induced Local Lesions in Genomes (TILLING) strategy based on mismatch digestion was used on families that carried the cpm genotype through many generations. Extensive scanning of 33 plastome genes and a few intergenic regions was conducted. Numerous polymorphisms were detected on both genic and intergenic regions. The detected polymorphisms can be accounted for by at least 61 independent mutational events. The vast majority of the polymorphisms originated in substitutions and small indels (insertions/deletions) in microsatellites. The rpl23 and the rps16 genes were the most polymorphic. Interestingly, the variation observed in the rpl23 gene consisted of several combinations of 5 different one nucleotide polymorphisms. Besides, 4 large indels that have direct repeats at both ends were also observed, which appear to be originated from recombinational events. The cpm mutation spectrum suggests that the CPM gene product is probably involved in plastome mismatch repair. The numerous subtle molecular changes that were localized in a wide range of plastome sites show the cpm as a valuable source of plastome variability for plant research and/or plant breeding. Moreover, the cpm mutant appears to be an interesting experimental material for investigating the mechanisms responsible for maintaining the stability of plant organelle DNA. Inst. de Genética "Ewald A. Favret"- IGEAF Fil: Landau, Alejandra Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Lencina, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Pacheco, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Prina, Alberto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina 2017-08-31T17:35:33Z 2017-08-31T17:35:33Z 2016 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:ar-repo/semantics/artículo http://hdl.handle.net/20.500.12123/1097 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4885237/ 0022-1503 (Print) 1465-7333 (Online) https://doi.org/10.1093/jhered/esw003 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Journal of heredity 107 (3) : 266–273. (May 2016) |
| spellingShingle | Genética Cebada Polimorfismo Cloroplasto Genetics Barley Polymorphism Chloroplasts Landau, Alejandra Mabel Lencina, Franco Pacheco, Maria Gabriela Prina, Alberto Raul Plastome mutations and recombination events in barley chloroplast mutator seedlings |
| title | Plastome mutations and recombination events in barley chloroplast mutator seedlings |
| title_full | Plastome mutations and recombination events in barley chloroplast mutator seedlings |
| title_fullStr | Plastome mutations and recombination events in barley chloroplast mutator seedlings |
| title_full_unstemmed | Plastome mutations and recombination events in barley chloroplast mutator seedlings |
| title_short | Plastome mutations and recombination events in barley chloroplast mutator seedlings |
| title_sort | plastome mutations and recombination events in barley chloroplast mutator seedlings |
| topic | Genética Cebada Polimorfismo Cloroplasto Genetics Barley Polymorphism Chloroplasts |
| url | http://hdl.handle.net/20.500.12123/1097 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4885237/ https://doi.org/10.1093/jhered/esw003 |
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