Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population
The genus Lotus includes a group of forage legume species including genotypes of agronomic interest and model species. In this work, an experimental hydroponic growth system allowed the discrimination of growth responses to ionic–osmotic stress in a population of recombinant inbred lines (RILs) deve...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
CSIRO Publishing
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/10936 https://www.publish.csiro.au/cp/CP13222 https://doi.org/10.1071/CP13222 |
| _version_ | 1855484684176195584 |
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| author | Quero, Gastón Gutíerrez, Lucía Lascano, Hernan Ramiro Monza, Jorge Sandal, Niels Borsani, Omar |
| author_browse | Borsani, Omar Gutíerrez, Lucía Lascano, Hernan Ramiro Monza, Jorge Quero, Gastón Sandal, Niels |
| author_facet | Quero, Gastón Gutíerrez, Lucía Lascano, Hernan Ramiro Monza, Jorge Sandal, Niels Borsani, Omar |
| author_sort | Quero, Gastón |
| collection | INTA Digital |
| description | The genus Lotus includes a group of forage legume species including genotypes of agronomic interest and model species. In this work, an experimental hydroponic growth system allowed the discrimination of growth responses to ionic–osmotic stress in a population of recombinant inbred lines (RILs) developed from L. japonicus × L. burttii and the identification of the associated quantitative trait loci (QTLs). The analyses led to the identification of eight QTLs: three for shoot growth localised on chromosome 3, 5 and 6; one for root growth on chromosome 1; three for total growth on chromosome 1, 4 and 5; and one associated with shoot/root ratio on chromosome 3. An interaction of QTL × stress condition was established and the effect of the environment quantified. In summary, it was established that the allele from L. burttii explained most responses to osmotic stress, while the alleles of L. japonicus explained the responses related to ionic stress conditions. Of 49 markers linked to all QTLs identified, 41 expressed superiority of the L. burttii parental allele in the osmotic stress condition, but when an iso-osmotic concentration of NaCl was applied, L. burttii lost superiority in 21 of these markers. This shows the superiority of the L. japonicus parental allele in ionic stress conditions. This study is the first report in which a RIL population of lotus is analysed with the aim of providing molecular markers associated with plant responses to ionic or osmotic stress. |
| format | Artículo |
| id | INTA10936 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | CSIRO Publishing |
| publisherStr | CSIRO Publishing |
| record_format | dspace |
| spelling | INTA109362021-12-17T20:07:11Z Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population Quero, Gastón Gutíerrez, Lucía Lascano, Hernan Ramiro Monza, Jorge Sandal, Niels Borsani, Omar Hydroponics Osmotic Stress Estrés Osmótico Cultivo Hidropónico Hydroponic Growth PEG Stress Salt Stress Lotus japonicus Lotus burttii Estrés Salino The genus Lotus includes a group of forage legume species including genotypes of agronomic interest and model species. In this work, an experimental hydroponic growth system allowed the discrimination of growth responses to ionic–osmotic stress in a population of recombinant inbred lines (RILs) developed from L. japonicus × L. burttii and the identification of the associated quantitative trait loci (QTLs). The analyses led to the identification of eight QTLs: three for shoot growth localised on chromosome 3, 5 and 6; one for root growth on chromosome 1; three for total growth on chromosome 1, 4 and 5; and one associated with shoot/root ratio on chromosome 3. An interaction of QTL × stress condition was established and the effect of the environment quantified. In summary, it was established that the allele from L. burttii explained most responses to osmotic stress, while the alleles of L. japonicus explained the responses related to ionic stress conditions. Of 49 markers linked to all QTLs identified, 41 expressed superiority of the L. burttii parental allele in the osmotic stress condition, but when an iso-osmotic concentration of NaCl was applied, L. burttii lost superiority in 21 of these markers. This shows the superiority of the L. japonicus parental allele in ionic stress conditions. This study is the first report in which a RIL population of lotus is analysed with the aim of providing molecular markers associated with plant responses to ionic or osmotic stress. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Quero, Gastón. Universidad de la República. Facultad de Agronomía. Laboratorio de Bioquímica; Uruguay Fil: Gutíerrez, Lucía. Universidad de la República. Facultad de Agronomía. Departamento de Biometría, Estadística y Computación; Uruguay Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Monza, Jorge. Universidad de la República. Facultad de Agronomía. Laboratorio de Bioquímica; Uruguay Fil: Sandal, Niels. Aarhus University. Centre for Carbohydrate Recognition and Signalling. Department of Molecular Biology and Genetics; Dinamarca Fil: Borsani, Omar. Universidad de la República. Facultad de Agronomía. Laboratorio de Bioquímica; Uruguay 2021-12-17T19:58:38Z 2021-12-17T19:58:38Z 2014-02-20 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10936 https://www.publish.csiro.au/cp/CP13222 1836-0947 1836-5795 (online) https://doi.org/10.1071/CP13222 eng info:eu-repo/semantics/restrictedAccess application/pdf CSIRO Publishing Crop and Pasture Science 65 (2) : 139-149 (2014) |
| spellingShingle | Hydroponics Osmotic Stress Estrés Osmótico Cultivo Hidropónico Hydroponic Growth PEG Stress Salt Stress Lotus japonicus Lotus burttii Estrés Salino Quero, Gastón Gutíerrez, Lucía Lascano, Hernan Ramiro Monza, Jorge Sandal, Niels Borsani, Omar Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population |
| title | Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population |
| title_full | Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population |
| title_fullStr | Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population |
| title_full_unstemmed | Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population |
| title_short | Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population |
| title_sort | identification of qtls for shoot and root growth under ionic osmotic stress in lotus using a ril population |
| topic | Hydroponics Osmotic Stress Estrés Osmótico Cultivo Hidropónico Hydroponic Growth PEG Stress Salt Stress Lotus japonicus Lotus burttii Estrés Salino |
| url | http://hdl.handle.net/20.500.12123/10936 https://www.publish.csiro.au/cp/CP13222 https://doi.org/10.1071/CP13222 |
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