The Activity of the Chloroplastic Ndh Complex Is Regulated by Phosphorylation of the NDH-F Subunit
Hydrogen peroxide (H2O2) induces increases, to different degrees, in transcripts, protein levels, and activity of the Ndh complex (EC 1.6.5.3). In the present work, we have compared the effects of relatively excess light, H2O2, dimethylthiourea (a scavenger of H2O2), and/or EGTA (a Ca2+chelator) on...
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| Format: | Artículo |
| Language: | Inglés |
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American Society of Plant Biologists
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/10935 https://academic.oup.com/plphys/article/132/1/256/6111831 https://doi.org/10.1104/pp.103.020321 |
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| author | Lascano, Hernan Ramiro Casano, Leonardo M. Martı́n, Mercedes Sabater, Bartolomé |
| author_browse | Casano, Leonardo M. Lascano, Hernan Ramiro Martı́n, Mercedes Sabater, Bartolomé |
| author_facet | Lascano, Hernan Ramiro Casano, Leonardo M. Martı́n, Mercedes Sabater, Bartolomé |
| author_sort | Lascano, Hernan Ramiro |
| collection | INTA Digital |
| description | Hydrogen peroxide (H2O2) induces increases, to different degrees, in transcripts, protein levels, and activity of the Ndh complex (EC 1.6.5.3). In the present work, we have compared the effects of relatively excess light, H2O2, dimethylthiourea (a scavenger of H2O2), and/or EGTA (a Ca2+chelator) on the activity and protein levels of the Ndh complex of barley (Hordeum vulgare cv Hassan) leaf segments. The results show the involvement of H2O2 in the modulation of both the protein level and activity of the Ndh complex and the participation of Ca2+ mainly in the activity regulation of pre-existing protein. Changes in Ndh complex activity could not be explained only by changes in Ndh protein levels, suggesting posttranslational modifications. Hence, we investigate the possible phosphorylation of the Ndh complex both in thylakoids and in the immunopurified Ndh complex using monoclonal phosphoamino acid antibodies. We demonstrate that the Ndh complex is phosphorylated in vivo at threonine residue(s) of the NDH-F polypeptide and that the level of phosphorylation is closely correlated with the Ndh complex activity. The emerging picture is that full activity of the Ndh complex is reached by phosphorylation of its NDH-F subunit in a H2O2- and Ca2+-mediated action. |
| format | Artículo |
| id | INTA10935 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | American Society of Plant Biologists |
| publisherStr | American Society of Plant Biologists |
| record_format | dspace |
| spelling | INTA109352021-12-17T19:55:29Z The Activity of the Chloroplastic Ndh Complex Is Regulated by Phosphorylation of the NDH-F Subunit Lascano, Hernan Ramiro Casano, Leonardo M. Martı́n, Mercedes Sabater, Bartolomé Hydrogen Peroxide Peróxido de Hidrógeno Fosforilación Phosphorylation Agua Oxigenada Hydrogen peroxide (H2O2) induces increases, to different degrees, in transcripts, protein levels, and activity of the Ndh complex (EC 1.6.5.3). In the present work, we have compared the effects of relatively excess light, H2O2, dimethylthiourea (a scavenger of H2O2), and/or EGTA (a Ca2+chelator) on the activity and protein levels of the Ndh complex of barley (Hordeum vulgare cv Hassan) leaf segments. The results show the involvement of H2O2 in the modulation of both the protein level and activity of the Ndh complex and the participation of Ca2+ mainly in the activity regulation of pre-existing protein. Changes in Ndh complex activity could not be explained only by changes in Ndh protein levels, suggesting posttranslational modifications. Hence, we investigate the possible phosphorylation of the Ndh complex both in thylakoids and in the immunopurified Ndh complex using monoclonal phosphoamino acid antibodies. We demonstrate that the Ndh complex is phosphorylated in vivo at threonine residue(s) of the NDH-F polypeptide and that the level of phosphorylation is closely correlated with the Ndh complex activity. The emerging picture is that full activity of the Ndh complex is reached by phosphorylation of its NDH-F subunit in a H2O2- and Ca2+-mediated action. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales. Argentina Fil: Lascano, Hernán Ramiro. Universidad de Alcalá de Henares. Departamento de Biología Vegetal; España Fil: Casano, Leonardo M. Universidad de Alcalá de Henares. Departamento de Biología Vegetal; España Fil: Martín, Mercedes. Universidad de Alcalá de Henares. Departamento de Biología Vegetal; España Fil: Sabater, Bartolomé. Universidad de Alcalá de Henares. Departamento de Biología Vegetal; España 2021-12-17T19:49:34Z 2021-12-17T19:49:34Z 2003-05-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10935 https://academic.oup.com/plphys/article/132/1/256/6111831 0032-0889 1532-2548 (online) https://doi.org/10.1104/pp.103.020321 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf American Society of Plant Biologists Plant Physiology 132 (1) : 256-262 (May 2003) |
| spellingShingle | Hydrogen Peroxide Peróxido de Hidrógeno Fosforilación Phosphorylation Agua Oxigenada Lascano, Hernan Ramiro Casano, Leonardo M. Martı́n, Mercedes Sabater, Bartolomé The Activity of the Chloroplastic Ndh Complex Is Regulated by Phosphorylation of the NDH-F Subunit |
| title | The Activity of the Chloroplastic Ndh Complex Is Regulated by Phosphorylation of the NDH-F Subunit |
| title_full | The Activity of the Chloroplastic Ndh Complex Is Regulated by Phosphorylation of the NDH-F Subunit |
| title_fullStr | The Activity of the Chloroplastic Ndh Complex Is Regulated by Phosphorylation of the NDH-F Subunit |
| title_full_unstemmed | The Activity of the Chloroplastic Ndh Complex Is Regulated by Phosphorylation of the NDH-F Subunit |
| title_short | The Activity of the Chloroplastic Ndh Complex Is Regulated by Phosphorylation of the NDH-F Subunit |
| title_sort | activity of the chloroplastic ndh complex is regulated by phosphorylation of the ndh f subunit |
| topic | Hydrogen Peroxide Peróxido de Hidrógeno Fosforilación Phosphorylation Agua Oxigenada |
| url | http://hdl.handle.net/20.500.12123/10935 https://academic.oup.com/plphys/article/132/1/256/6111831 https://doi.org/10.1104/pp.103.020321 |
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