Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition
The short-term (2 h) effects of salt stress (50 and 150 mM NaCl) on early events of soybean– Bradyrhizobium japonicum (rhizobia) interaction were analysed, determining the following parameters in root hair with or without calcium addition: deformation, apoplastic superoxide radical production (O2⚫–)...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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CSIRO Publishing
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/10933 https://www.publish.csiro.au/fp/FP13085 https://doi.org/10.1071/FP13085 |
| _version_ | 1855036479658524672 |
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| author | Muñoz, Nacira Belen Rodriguez, Marianela Robert, German Lascano, Hernan Ramiro |
| author_browse | Lascano, Hernan Ramiro Muñoz, Nacira Belen Robert, German Rodriguez, Marianela |
| author_facet | Muñoz, Nacira Belen Rodriguez, Marianela Robert, German Lascano, Hernan Ramiro |
| author_sort | Muñoz, Nacira Belen |
| collection | INTA Digital |
| description | The short-term (2 h) effects of salt stress (50 and 150 mM NaCl) on early events of soybean– Bradyrhizobium japonicum (rhizobia) interaction were analysed, determining the following parameters in root hair with or without calcium addition: deformation, apoplastic superoxide radical production (O2⚫–), root hair death and sodium/potassium ion content. We also analysed whether this short-term salt stress influenced later formation of crown and noncrown nodules, determining the number and weight of nodules. The negative effect of salt stress on these characters was attenuated by the addition of 5 mM CaCl2. We also analysed the expression of pathogenesis-related proteins (PRP) genes PR-1, PR-2, PR-3, and four isoforms of PR-5. The expression of PR-2 increased under saline conditions and decreased in osmotic treatment and saline treatment supplemented with calcium in the presence of the symbiont. The changes in PR-2 expression levels, together with the death of root hairs provide a possible mechanism for the inhibition of infection by the symbiont under salinity, and suggests a possible overlap with responses to plant pathogens. |
| format | info:ar-repo/semantics/artículo |
| id | INTA10933 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | CSIRO Publishing |
| publisherStr | CSIRO Publishing |
| record_format | dspace |
| spelling | INTA109332021-12-17T19:36:16Z Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition Muñoz, Nacira Belen Rodriguez, Marianela Robert, German Lascano, Hernan Ramiro Root Nodulation Osmotic Stress Estrés Osmótico Nodulación Bradyrhizobium japonicum Ionic Homeostasis Nodulation Pathogenic-Related Proteins Symbiotic Interaction Estrés Salino The short-term (2 h) effects of salt stress (50 and 150 mM NaCl) on early events of soybean– Bradyrhizobium japonicum (rhizobia) interaction were analysed, determining the following parameters in root hair with or without calcium addition: deformation, apoplastic superoxide radical production (O2⚫–), root hair death and sodium/potassium ion content. We also analysed whether this short-term salt stress influenced later formation of crown and noncrown nodules, determining the number and weight of nodules. The negative effect of salt stress on these characters was attenuated by the addition of 5 mM CaCl2. We also analysed the expression of pathogenesis-related proteins (PRP) genes PR-1, PR-2, PR-3, and four isoforms of PR-5. The expression of PR-2 increased under saline conditions and decreased in osmotic treatment and saline treatment supplemented with calcium in the presence of the symbiont. The changes in PR-2 expression levels, together with the death of root hairs provide a possible mechanism for the inhibition of infection by the symbiont under salinity, and suggests a possible overlap with responses to plant pathogens. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Muñoz, Nacira Belen. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Robert, German. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales. Argentina Fil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina 2021-12-17T19:30:20Z 2021-12-17T19:30:20Z 2013-09-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10933 https://www.publish.csiro.au/fp/FP13085 1445-4408 1445-4416 (online) https://doi.org/10.1071/FP13085 eng info:eu-repo/semantics/restrictedAccess application/pdf CSIRO Publishing Functional Plant Biology 41 (1) : 96-105 ( 2013) |
| spellingShingle | Root Nodulation Osmotic Stress Estrés Osmótico Nodulación Bradyrhizobium japonicum Ionic Homeostasis Nodulation Pathogenic-Related Proteins Symbiotic Interaction Estrés Salino Muñoz, Nacira Belen Rodriguez, Marianela Robert, German Lascano, Hernan Ramiro Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition |
| title | Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition |
| title_full | Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition |
| title_fullStr | Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition |
| title_full_unstemmed | Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition |
| title_short | Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition |
| title_sort | negative short term salt effects on the soybean bradyrhizobium japonicum interaction and partial reversion by calcium addition |
| topic | Root Nodulation Osmotic Stress Estrés Osmótico Nodulación Bradyrhizobium japonicum Ionic Homeostasis Nodulation Pathogenic-Related Proteins Symbiotic Interaction Estrés Salino |
| url | http://hdl.handle.net/20.500.12123/10933 https://www.publish.csiro.au/fp/FP13085 https://doi.org/10.1071/FP13085 |
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