Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus
The morphology and physiology of the oogenesis have been well studied in the vector of Chagas disease Rhodnius prolixus. However, the molecular interactions that regulate the process of egg formation, key for the reproductive cycle of the vector, is still largely unknown. In order to understand the...
| Main Authors: | , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/10896 https://link.springer.com/article/10.1007%2Fs00427-021-00673-0 https://doi.org/10.1007/s00427-021-00673-0 |
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| author | Pascual, Agustina Vilardo, Emiliano Salvador Taibo, Catalina Beatriz Sabio Y Garcia, Julia Veronica Rivera Pomar, Rolando |
| author_browse | Pascual, Agustina Rivera Pomar, Rolando Sabio Y Garcia, Julia Veronica Taibo, Catalina Beatriz Vilardo, Emiliano Salvador |
| author_facet | Pascual, Agustina Vilardo, Emiliano Salvador Taibo, Catalina Beatriz Sabio Y Garcia, Julia Veronica Rivera Pomar, Rolando |
| author_sort | Pascual, Agustina |
| collection | INTA Digital |
| description | The morphology and physiology of the oogenesis have been well studied in the vector of Chagas disease Rhodnius prolixus. However, the molecular interactions that regulate the process of egg formation, key for the reproductive cycle of the vector, is still largely unknown. In order to understand the molecular and cellular basis of the oogenesis, we examined the function of the gene Bicaudal C (BicC) during oogenesis and early development of R. prolixus. We show that R. prolixus BicC (Rp-BicC) gene is expressed in the germarium, with cytoplasmic distribution, as well as in the follicular epithelium of the developing oocytes. RNAi silencing of Rp-BicC resulted in sterile females that lay few, small, non-viable eggs. The ovaries are reduced in size and show a disarray of the follicular epithelium. This indicates that Rp-BicC has a central role in the regulation of oogenesis. Although the follicular cells are able to form the chorion, the uptake of vitelline by the oocytes is compromised. We show evidence that the polarity of the follicular epithelium and the endocytic pathway, which are crucial for the proper yolk deposition, are affected. This study provides insights into the molecular mechanisms underlying oocyte development and show that Rp-BicC is important for de developmental of the egg and, therefore, a key player in the reproduction of this insect. |
| format | Artículo |
| id | INTA10896 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA108962022-08-12T13:52:41Z Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus Pascual, Agustina Vilardo, Emiliano Salvador Taibo, Catalina Beatriz Sabio Y Garcia, Julia Veronica Rivera Pomar, Rolando Oogénesis Rhodnius prolixus Desarrollo Embrionario Vitelogénesis Oogenesis Embryonic Development Vitellogenesis Bicaudal C The morphology and physiology of the oogenesis have been well studied in the vector of Chagas disease Rhodnius prolixus. However, the molecular interactions that regulate the process of egg formation, key for the reproductive cycle of the vector, is still largely unknown. In order to understand the molecular and cellular basis of the oogenesis, we examined the function of the gene Bicaudal C (BicC) during oogenesis and early development of R. prolixus. We show that R. prolixus BicC (Rp-BicC) gene is expressed in the germarium, with cytoplasmic distribution, as well as in the follicular epithelium of the developing oocytes. RNAi silencing of Rp-BicC resulted in sterile females that lay few, small, non-viable eggs. The ovaries are reduced in size and show a disarray of the follicular epithelium. This indicates that Rp-BicC has a central role in the regulation of oogenesis. Although the follicular cells are able to form the chorion, the uptake of vitelline by the oocytes is compromised. We show evidence that the polarity of the follicular epithelium and the endocytic pathway, which are crucial for the proper yolk deposition, are affected. This study provides insights into the molecular mechanisms underlying oocyte development and show that Rp-BicC is important for de developmental of the egg and, therefore, a key player in the reproduction of this insect. Centro de Investigación en Ciencias Veterinarias y Agronómicas Fil: Pascual, Agustina. Universidad Nacional del Norte de la Provincia de Buenos Aires. Centro de Bioinvestigaciones; Argentina Fil: Pascual, Agustina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Centro de Bioinvestigaciones; Argentina Fil: Vilardo, Emiliano Salvador. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro Regional de Estudios Genómicos; Argentina Fil: Taibo, Catalina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; Argentina Fil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; Argentina Fil: Rivera Pomar, Rolando. Universidad Nacional del Norte de la Provincia de Buenos Aires. Centro de Bioinvestigaciones; Argentina Fil: Rivera Pomar, Rolando. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Centro de Bioinvestigaciones; Argentina Fil: Rivera Pomar, Rolando. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro Regional de Estudios Genómicos; Argentina 2021-12-13T14:48:46Z 2021-12-13T14:48:46Z 2021-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10896 https://link.springer.com/article/10.1007%2Fs00427-021-00673-0 1432-041X 0949-944X https://doi.org/10.1007/s00427-021-00673-0 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Development Genes and Evolution 231 : 33-45. (2021) |
| spellingShingle | Oogénesis Rhodnius prolixus Desarrollo Embrionario Vitelogénesis Oogenesis Embryonic Development Vitellogenesis Bicaudal C Pascual, Agustina Vilardo, Emiliano Salvador Taibo, Catalina Beatriz Sabio Y Garcia, Julia Veronica Rivera Pomar, Rolando Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus |
| title | Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus |
| title_full | Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus |
| title_fullStr | Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus |
| title_full_unstemmed | Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus |
| title_short | Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus |
| title_sort | bicaudal c is required for the function of the follicular epithelium during oogenesis in rhodnius prolixus |
| topic | Oogénesis Rhodnius prolixus Desarrollo Embrionario Vitelogénesis Oogenesis Embryonic Development Vitellogenesis Bicaudal C |
| url | http://hdl.handle.net/20.500.12123/10896 https://link.springer.com/article/10.1007%2Fs00427-021-00673-0 https://doi.org/10.1007/s00427-021-00673-0 |
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