Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry
In the last 10 years, Salmonella Heidelberg has been extensively isolated from poultry in several countries. In this context, molecular characterization is essential to understand whether the strains have entered the farms from a single or several sources. Thus, the aim of this study was to determin...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/10883 https://onlinelibrary.wiley.com/doi/10.1111/zph.12819 https://doi.org/10.1111/zph.12819 |
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| author | Dominguez, Johanna Elizabeth Viñas, María Rosa Herrera, Mariana Moroni, Mirian Gutkind, Gabriel Omar Mercado, Elsa Cristina Di Conza, José Alejandro Chacana, Pablo |
| author_browse | Chacana, Pablo Di Conza, José Alejandro Dominguez, Johanna Elizabeth Gutkind, Gabriel Omar Herrera, Mariana Mercado, Elsa Cristina Moroni, Mirian Viñas, María Rosa |
| author_facet | Dominguez, Johanna Elizabeth Viñas, María Rosa Herrera, Mariana Moroni, Mirian Gutkind, Gabriel Omar Mercado, Elsa Cristina Di Conza, José Alejandro Chacana, Pablo |
| author_sort | Dominguez, Johanna Elizabeth |
| collection | INTA Digital |
| description | In the last 10 years, Salmonella Heidelberg has been extensively isolated from poultry in several countries. In this context, molecular characterization is essential to understand whether the strains have entered the farms from a single or several sources. Thus, the aim of this study was to determine the genetic relationship and antimicrobial susceptibility of S. Heidelberg strains isolated between 2011 and 2012 from broiler farms belonging to three integrated poultry companies located in Argentina. The genetic relatedness of the S. Heidelberg isolates was determined by pulsed-field gel electrophoresis (PFGE), and resistance to 21 antimicrobials was determined by the disc diffusion method. The isolates were assigned to four PFGE patterns. Most of the strains showed 100% similarity and belonged to the same integrated poultry company. This PFGE pattern was also prevalent in S. Heidelberg strains isolated from humans in several provinces of Argentina, which suggests an epidemiological association between human and poultry strains. All the isolates were classified as multidrug-resistant (MDR), and no clear relationship was observed between PFGE and resistance patterns. S. Heidelberg strains may circulate among farms from the same integrated company due to common sources of contamination. To guarantee the safety of the poultry product for the consumers, holistic approaches including surveillance of Salmonella throughout the production chain together with control measures are crucial. |
| format | info:ar-repo/semantics/artículo |
| id | INTA10883 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA108832021-12-10T14:21:47Z Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry Dominguez, Johanna Elizabeth Viñas, María Rosa Herrera, Mariana Moroni, Mirian Gutkind, Gabriel Omar Mercado, Elsa Cristina Di Conza, José Alejandro Chacana, Pablo Aves de Corral Salmonella Genética Molecular Resistencia a los Antimicrobianos Poultry Molecular Genetics Antimicrobial Resistance In the last 10 years, Salmonella Heidelberg has been extensively isolated from poultry in several countries. In this context, molecular characterization is essential to understand whether the strains have entered the farms from a single or several sources. Thus, the aim of this study was to determine the genetic relationship and antimicrobial susceptibility of S. Heidelberg strains isolated between 2011 and 2012 from broiler farms belonging to three integrated poultry companies located in Argentina. The genetic relatedness of the S. Heidelberg isolates was determined by pulsed-field gel electrophoresis (PFGE), and resistance to 21 antimicrobials was determined by the disc diffusion method. The isolates were assigned to four PFGE patterns. Most of the strains showed 100% similarity and belonged to the same integrated poultry company. This PFGE pattern was also prevalent in S. Heidelberg strains isolated from humans in several provinces of Argentina, which suggests an epidemiological association between human and poultry strains. All the isolates were classified as multidrug-resistant (MDR), and no clear relationship was observed between PFGE and resistance patterns. S. Heidelberg strains may circulate among farms from the same integrated company due to common sources of contamination. To guarantee the safety of the poultry product for the consumers, holistic approaches including surveillance of Salmonella throughout the production chain together with control measures are crucial. Instituto de Patobiología Fil: Dominguez, Johana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Fil: Dominguez, Johana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Viñas, María Rosa. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán (ANLIS-Malbrán). Servicios de Enterobacterias; Argentina Fil: Herrera, Mariana. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Dirección General de Laboratorios y Control Técnico; Argentina Fil: Moroni, Mirian. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán (ANLIS-Malbrán). Servicios de Enterobacterias; Argentina Fil: Gutkind, Gabriel O. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina Fil: Gutkind, Gabriel O. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Di Conza, José A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina Fil: Di Conza, José A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-12-10T14:17:42Z 2021-12-10T14:17:42Z 2021-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10883 https://onlinelibrary.wiley.com/doi/10.1111/zph.12819 1863-2378 https://doi.org/10.1111/zph.12819 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Zoonoses and Public Health 68 (4) : 309-315 (June 2021) |
| spellingShingle | Aves de Corral Salmonella Genética Molecular Resistencia a los Antimicrobianos Poultry Molecular Genetics Antimicrobial Resistance Dominguez, Johanna Elizabeth Viñas, María Rosa Herrera, Mariana Moroni, Mirian Gutkind, Gabriel Omar Mercado, Elsa Cristina Di Conza, José Alejandro Chacana, Pablo Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry |
| title | Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry |
| title_full | Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry |
| title_fullStr | Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry |
| title_full_unstemmed | Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry |
| title_short | Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry |
| title_sort | molecular characterization and antimicrobial resistance profiles of salmonella heidelberg isolates from poultry |
| topic | Aves de Corral Salmonella Genética Molecular Resistencia a los Antimicrobianos Poultry Molecular Genetics Antimicrobial Resistance |
| url | http://hdl.handle.net/20.500.12123/10883 https://onlinelibrary.wiley.com/doi/10.1111/zph.12819 https://doi.org/10.1111/zph.12819 |
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