Volatile compounds released by maize grains and silks in response to infection by Fusarium verticillioides and its association with pathogen resistance

Many plants respond to fungal infections by producing and/or emitting a specific blend of oxylipins, some of them through the lipoxygenase (LOX) pathway. In vitro bioassays revealed that volatiles from silks and grains of a set of six maize genotypes with variable resistance to Fusarium verticillioi...

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Autores principales: Fauguel, Carolina Maria, Campos Bermudez, Valeria Alina, Iglesias, Juliana, Fernandez, Mariana, Farroni, Abel Eduardo, Andreo, Carlos Santiago, Presello, Daniel Alberto
Formato: info:eu-repo/semantics/article
Lenguaje:Inglés
Publicado: 2017
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/1085
http://onlinelibrary.wiley.com/doi/10.1111/ppa.12663/epdf
https://doi.org/10.1111/ppa.12663
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author Fauguel, Carolina Maria
Campos Bermudez, Valeria Alina
Iglesias, Juliana
Fernandez, Mariana
Farroni, Abel Eduardo
Andreo, Carlos Santiago
Presello, Daniel Alberto
author_browse Andreo, Carlos Santiago
Campos Bermudez, Valeria Alina
Farroni, Abel Eduardo
Fauguel, Carolina Maria
Fernandez, Mariana
Iglesias, Juliana
Presello, Daniel Alberto
author_facet Fauguel, Carolina Maria
Campos Bermudez, Valeria Alina
Iglesias, Juliana
Fernandez, Mariana
Farroni, Abel Eduardo
Andreo, Carlos Santiago
Presello, Daniel Alberto
author_sort Fauguel, Carolina Maria
collection INTA Digital
description Many plants respond to fungal infections by producing and/or emitting a specific blend of oxylipins, some of them through the lipoxygenase (LOX) pathway. In vitro bioassays revealed that volatiles from silks and grains of a set of six maize genotypes with variable resistance to Fusarium verticillioides affected fungal growth in different ways. Analyses by solid phase microextraction and GC-MS showed different volatile profiles in silks and grains of each genotype. Susceptible genotypes with higher concentrations of precursor polyunsaturated fatty acids produced more volatiles, mainly C9 compounds, in comparison to moderately resistant ones. Real-time PCR from several lipoxygenase transcripts revealed that levels of gene expression depended on the genotype and the inoculation level and suggested that F. verticillioides could use compounds from the 9-LOX pathway to promote infection in grains. On the other hand, volatiles produced by the 13-LOX pathway were more important in moderately resistant genotypes and could be associated to field resistance. Results from this set of genotypes indicate that LOX-regulated volatile compounds might be important in F. verticillioides infection and should be targeted in further research to understand disease resistance.
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spelling INTA10852019-06-06T17:45:33Z Volatile compounds released by maize grains and silks in response to infection by Fusarium verticillioides and its association with pathogen resistance Fauguel, Carolina Maria Campos Bermudez, Valeria Alina Iglesias, Juliana Fernandez, Mariana Farroni, Abel Eduardo Andreo, Carlos Santiago Presello, Daniel Alberto Enfermedades de las Plantas Maíz Fusarium Resistencia a Agentes Dañinos Compuesto Volátil Maize Plant Diseases Resistance to Injurious Factors Volatile Compounds Fusarium Verticillioides Many plants respond to fungal infections by producing and/or emitting a specific blend of oxylipins, some of them through the lipoxygenase (LOX) pathway. In vitro bioassays revealed that volatiles from silks and grains of a set of six maize genotypes with variable resistance to Fusarium verticillioides affected fungal growth in different ways. Analyses by solid phase microextraction and GC-MS showed different volatile profiles in silks and grains of each genotype. Susceptible genotypes with higher concentrations of precursor polyunsaturated fatty acids produced more volatiles, mainly C9 compounds, in comparison to moderately resistant ones. Real-time PCR from several lipoxygenase transcripts revealed that levels of gene expression depended on the genotype and the inoculation level and suggested that F. verticillioides could use compounds from the 9-LOX pathway to promote infection in grains. On the other hand, volatiles produced by the 13-LOX pathway were more important in moderately resistant genotypes and could be associated to field resistance. Results from this set of genotypes indicate that LOX-regulated volatile compounds might be important in F. verticillioides infection and should be targeted in further research to understand disease resistance. EEA Pergamino Fil: Fauguel, Carolina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina Fil: Campos Bermudez, Valeria A. Centro de Estudios Fotosintéticos y Bioquíımicos; Argentina Fil: Iglesias, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina Fil: Fernandez, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina Fil: Farroni, Abel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina Fil: Andreo, C.S. Centro de Estudios Fotosintéticos y Bioquíımicos; Argentina Fil: Presello, Daniel Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina 2017-08-31T11:46:30Z 2017-08-31T11:46:30Z 2016 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:ar-repo/semantics/artículo http://hdl.handle.net/20.500.12123/1085 http://onlinelibrary.wiley.com/doi/10.1111/ppa.12663/epdf 0032-0862 (Print) 1365-3059 (Online) https://doi.org/10.1111/ppa.12663 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Plant pathology 66 (7) : 1128-1138. (September 2017)
spellingShingle Enfermedades de las Plantas
Maíz
Fusarium
Resistencia a Agentes Dañinos
Compuesto Volátil
Maize
Plant Diseases
Resistance to Injurious Factors
Volatile Compounds
Fusarium Verticillioides
Fauguel, Carolina Maria
Campos Bermudez, Valeria Alina
Iglesias, Juliana
Fernandez, Mariana
Farroni, Abel Eduardo
Andreo, Carlos Santiago
Presello, Daniel Alberto
Volatile compounds released by maize grains and silks in response to infection by Fusarium verticillioides and its association with pathogen resistance
title Volatile compounds released by maize grains and silks in response to infection by Fusarium verticillioides and its association with pathogen resistance
title_full Volatile compounds released by maize grains and silks in response to infection by Fusarium verticillioides and its association with pathogen resistance
title_fullStr Volatile compounds released by maize grains and silks in response to infection by Fusarium verticillioides and its association with pathogen resistance
title_full_unstemmed Volatile compounds released by maize grains and silks in response to infection by Fusarium verticillioides and its association with pathogen resistance
title_short Volatile compounds released by maize grains and silks in response to infection by Fusarium verticillioides and its association with pathogen resistance
title_sort volatile compounds released by maize grains and silks in response to infection by fusarium verticillioides and its association with pathogen resistance
topic Enfermedades de las Plantas
Maíz
Fusarium
Resistencia a Agentes Dañinos
Compuesto Volátil
Maize
Plant Diseases
Resistance to Injurious Factors
Volatile Compounds
Fusarium Verticillioides
url http://hdl.handle.net/20.500.12123/1085
http://onlinelibrary.wiley.com/doi/10.1111/ppa.12663/epdf
https://doi.org/10.1111/ppa.12663
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