Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars
Background: Biotechnological breeding of elite sugarcane cultivars is currently limited because of the difficulty of regenerating plants by tissue culture. Here, we report that commercially elite sugarcane genotypes, which are adapted to Argentinian agro-ecological conditions, are capable of being r...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/10847 https://jgeb.springeropen.com/articles/10.1186/s43141-021-00270-8 https://doi.org/10.1186/s43141-021-00270-8 |
| _version_ | 1855484668181217280 |
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| author | Di Pauli, Valentina Fontana, Paola Daniela Lewi, Dalia Marcela Felipe, Arturo Erazzu, Luis Ernesto |
| author_browse | Di Pauli, Valentina Erazzu, Luis Ernesto Felipe, Arturo Fontana, Paola Daniela Lewi, Dalia Marcela |
| author_facet | Di Pauli, Valentina Fontana, Paola Daniela Lewi, Dalia Marcela Felipe, Arturo Erazzu, Luis Ernesto |
| author_sort | Di Pauli, Valentina |
| collection | INTA Digital |
| description | Background: Biotechnological breeding of elite sugarcane cultivars is currently limited because of the difficulty of regenerating plants by tissue culture. Here, we report that commercially elite sugarcane genotypes, which are adapted to Argentinian agro-ecological conditions, are capable of being regenerated via indirect somatic embryogenesis. Leaf rolls of five elite genotypes were cultured following two callus induction protocols using different concentrations of 2,4-D as the growth regulator. Embryogenic calluses were regenerated under light conditions. Regenerated plants were subsequently acclimatized in the greenhouse under two acclimatization procedures before being transplanted to the field.
Results: Four of the five genotypes were able to form somatic embryos following the two induction protocols. The variables related to embryogenic callus production were influenced by the interaction between genotype and culture conditions. For plant regeneration, the embryogenic calluses were further cultured on an IBA-supplemented medium, where we observed a high genotype dependence. Calluses from the four cultivars regenerated a good number of plants. With the procedures described here, we obtained more than 90% of well-acclimatized plants both in the greenhouse and in the field.
Conclusions: This protocol provides a simple way to regenerate sugarcane plants through indirect somatic embryogenesis. Also, the results confirm that tissue culture ability is highly genotype-dependent in sugarcane. Our findings suggest that these elite cultivars could be good candidates for biotechnological breeding. |
| format | Artículo |
| id | INTA10847 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA108472021-12-03T13:42:00Z Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars Di Pauli, Valentina Fontana, Paola Daniela Lewi, Dalia Marcela Felipe, Arturo Erazzu, Luis Ernesto Caña de Azúcar Variedades Biotecnología Embriogénesis Somática Genotipos Sugar Cane Varieties Biotechnology Somatic Embryogenesis Genotypes Embryogenesis Background: Biotechnological breeding of elite sugarcane cultivars is currently limited because of the difficulty of regenerating plants by tissue culture. Here, we report that commercially elite sugarcane genotypes, which are adapted to Argentinian agro-ecological conditions, are capable of being regenerated via indirect somatic embryogenesis. Leaf rolls of five elite genotypes were cultured following two callus induction protocols using different concentrations of 2,4-D as the growth regulator. Embryogenic calluses were regenerated under light conditions. Regenerated plants were subsequently acclimatized in the greenhouse under two acclimatization procedures before being transplanted to the field. Results: Four of the five genotypes were able to form somatic embryos following the two induction protocols. The variables related to embryogenic callus production were influenced by the interaction between genotype and culture conditions. For plant regeneration, the embryogenic calluses were further cultured on an IBA-supplemented medium, where we observed a high genotype dependence. Calluses from the four cultivars regenerated a good number of plants. With the procedures described here, we obtained more than 90% of well-acclimatized plants both in the greenhouse and in the field. Conclusions: This protocol provides a simple way to regenerate sugarcane plants through indirect somatic embryogenesis. Also, the results confirm that tissue culture ability is highly genotype-dependent in sugarcane. Our findings suggest that these elite cultivars could be good candidates for biotechnological breeding. EEA Famaillá Fil: Di Pauli, Valentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Fontana, Paola Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Felipe, Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Erazzú, Luis E. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina. 2021-12-03T13:39:43Z 2021-12-03T13:39:43Z 2021-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10847 https://jgeb.springeropen.com/articles/10.1186/s43141-021-00270-8 1687-157X 2090-5920 https://doi.org/10.1186/s43141-021-00270-8 eng info:eu-repograntAgreement/INTA/PNIND-1108063/AR./Desarrollo y aplicación de nuevas herramientas tecnológicas para caracterización y generación de materiales genéticos info:eu-repograntAgreement/INTA/PNBIO-1131024/AR./Desarrollo de sistemas alternativos de generación y utilización de variabilidad genética y su aplicación al mejoramiento de los cultivos. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Journal of Genetic Engineering and Biotechnology 19 : Article number: 171 (2021) |
| spellingShingle | Caña de Azúcar Variedades Biotecnología Embriogénesis Somática Genotipos Sugar Cane Varieties Biotechnology Somatic Embryogenesis Genotypes Embryogenesis Di Pauli, Valentina Fontana, Paola Daniela Lewi, Dalia Marcela Felipe, Arturo Erazzu, Luis Ernesto Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars |
| title | Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars |
| title_full | Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars |
| title_fullStr | Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars |
| title_full_unstemmed | Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars |
| title_short | Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars |
| title_sort | optimized somatic embryogenesis and plant regeneration in elite argentinian sugarcane saccharum spp cultivars |
| topic | Caña de Azúcar Variedades Biotecnología Embriogénesis Somática Genotipos Sugar Cane Varieties Biotechnology Somatic Embryogenesis Genotypes Embryogenesis |
| url | http://hdl.handle.net/20.500.12123/10847 https://jgeb.springeropen.com/articles/10.1186/s43141-021-00270-8 https://doi.org/10.1186/s43141-021-00270-8 |
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