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New entomopathogenic strain of Bacillus thuringiensis is able to solubilize different sources of inorganic phosphates

Phosphate-solubilizing strain INTA 293-1 from a soil sample of Corrientes province (Argentina) was identified as Bacillus thuringiensis based on its faculty to produce crystalline inclusions during sporulation and 16S rDNA sequence similarity. INTA 293-1 was found to produce bipyramidal and cuboidal...

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Main Authors: Sauka, Diego Hernan, Piccinetti, Carlos Fabian, Vallejo, Daniela Adriana, Onco, Maria Ines, Perez, Melisa Paula, Benintende, Graciela Beatriz
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Elsevier 2021
Subjects:
Bacillus thuringiensis
Plagas de Plantas
Bacterias Entomógenas
Plant Pests
Pest Control
Entomogenous Bacteria
Control de Plagas
Bacterias solubilizadoras de fosfato
Bacterias Entomopatógenas
Online Access:http://hdl.handle.net/20.500.12123/10827
https://www.sciencedirect.com/science/article/pii/S092913932030768X
https://doi.org/10.1016/j.apsoil.2020.103839
Summary:Phosphate-solubilizing strain INTA 293-1 from a soil sample of Corrientes province (Argentina) was identified as Bacillus thuringiensis based on its faculty to produce crystalline inclusions during sporulation and 16S rDNA sequence similarity. INTA 293-1 was found to produce bipyramidal and cuboidal crystals, containing major proteins of ca. 130 and 65 kDa respectively, resembling those present in Lepidoptera- and Diptera-active strains. Accordingly, INTA 293-1 showed insecticidal activity against lepidopteran and dipteran larvae. No insecticidal activity nor evident effects on the normal development of coleopteran larvae or nematodes were observed. Screening of cry, cyt and vip genes showed that INTA 293-1 contains cry1Ac, cry1Ia, cry2Aa, cry2Ab and vip3Aa genes. Furthermore, this new entomopathogenic strain of B. thuringiensis was able to solubilize different sources of inorganic phosphates in liquid media following similar solubilization kinetics. INTA 293-1 was effective in solubilizing hydroxyapatite, patagonic phosphate rock, and to a lesser extent the aluminium phosphate. The differential behaviour between them may be explained by differences in INTA 293-1 survival. Phosphate solubilization was associated with pH reduction. This study reveals a little-explored aspect of B. thuringiensis and paves the way for the development of new agrobiological products with multiple modes of action.

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