Pollen-specific aquaporins NIP4;1 and NIP4;2 are required for pollen development and pollination in Arabidopsis thaliana
In flowers with dry stigmas, pollen development, pollination, and pollen tube growth require spatial and temporal regulation of water and nutrient transport. To better understand the molecular mechanisms involved in reproductive processes, we characterized NIP4;1 and NIP4;2, two pollen-specific aqua...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1080 http://www.plantcell.org/content/plantcell/28/5/1053.full.pdf https://doi.org/10.1105/tpc.15.00776 |
| _version_ | 1855482824461647872 |
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| author | Perez Di Giorgio, Juliana Andrea Bienert, Gerd Patrick Ayub, Nicolás Daniel Yaneff, Agustín Barberini, María Laura Mecchia, Martín Alejandro Amodeo, Gabriela Soto, Gabriela Cinthia Muschietti, Jorge Prometeo |
| author_browse | Amodeo, Gabriela Ayub, Nicolás Daniel Barberini, María Laura Bienert, Gerd Patrick Mecchia, Martín Alejandro Muschietti, Jorge Prometeo Perez Di Giorgio, Juliana Andrea Soto, Gabriela Cinthia Yaneff, Agustín |
| author_facet | Perez Di Giorgio, Juliana Andrea Bienert, Gerd Patrick Ayub, Nicolás Daniel Yaneff, Agustín Barberini, María Laura Mecchia, Martín Alejandro Amodeo, Gabriela Soto, Gabriela Cinthia Muschietti, Jorge Prometeo |
| author_sort | Perez Di Giorgio, Juliana Andrea |
| collection | INTA Digital |
| description | In flowers with dry stigmas, pollen development, pollination, and pollen tube growth require spatial and temporal regulation of water and nutrient transport. To better understand the molecular mechanisms involved in reproductive processes, we characterized NIP4;1 and NIP4;2, two pollen-specific aquaporins of Arabidopsis thaliana. NIP4;1 and NIP4;2 are paralogs found exclusively in the angiosperm lineage. Although they have 84% amino acid identity, they displayed different expression patterns. NIP4;1 has low expression levels in mature pollen, while NIP4;2 expression peaks during pollen tube growth. Additionally, NIP4;1pro:GUS flowers showed GUS activity in mature pollen and pollen tubes, whereas NIP4;2pro:GUS flowers only in pollen tubes. Single T-DNA mutants and double artificial microRNA knockdowns had fewer seeds per silique and reduced pollen germination and pollen tube length. Transport assays in oocytes showed NIP4;1 and NIP4;2 function as water and nonionic channels. We also found that NIP4;1 and NIP4;2 C termini are phosphorylated by a pollen-specific CPK that modifies their water permeability. Survival assays in yeast indicated that NIP4;1 also transports ammonia, urea, boric acid, and H2O2. Thus, we propose that aquaporins NIP4;1 and NIP4;2 are exclusive components of the reproductive apparatus of angiosperms with partially redundant roles in pollen development and pollination. |
| format | Artículo |
| id | INTA1080 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA10802018-02-26T12:07:24Z Pollen-specific aquaporins NIP4;1 and NIP4;2 are required for pollen development and pollination in Arabidopsis thaliana Perez Di Giorgio, Juliana Andrea Bienert, Gerd Patrick Ayub, Nicolás Daniel Yaneff, Agustín Barberini, María Laura Mecchia, Martín Alejandro Amodeo, Gabriela Soto, Gabriela Cinthia Muschietti, Jorge Prometeo Arabidopsis Thaliana Polinización Polen Pollination Pollen In flowers with dry stigmas, pollen development, pollination, and pollen tube growth require spatial and temporal regulation of water and nutrient transport. To better understand the molecular mechanisms involved in reproductive processes, we characterized NIP4;1 and NIP4;2, two pollen-specific aquaporins of Arabidopsis thaliana. NIP4;1 and NIP4;2 are paralogs found exclusively in the angiosperm lineage. Although they have 84% amino acid identity, they displayed different expression patterns. NIP4;1 has low expression levels in mature pollen, while NIP4;2 expression peaks during pollen tube growth. Additionally, NIP4;1pro:GUS flowers showed GUS activity in mature pollen and pollen tubes, whereas NIP4;2pro:GUS flowers only in pollen tubes. Single T-DNA mutants and double artificial microRNA knockdowns had fewer seeds per silique and reduced pollen germination and pollen tube length. Transport assays in oocytes showed NIP4;1 and NIP4;2 function as water and nonionic channels. We also found that NIP4;1 and NIP4;2 C termini are phosphorylated by a pollen-specific CPK that modifies their water permeability. Survival assays in yeast indicated that NIP4;1 also transports ammonia, urea, boric acid, and H2O2. Thus, we propose that aquaporins NIP4;1 and NIP4;2 are exclusive components of the reproductive apparatus of angiosperms with partially redundant roles in pollen development and pollination. Inst. de Genética "Ewald A. Favret"- IGEAF Fil: Perez Di Giorgio, Juliana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina Fil: Bienert, Gerd Patrick. Leibniz Institute of Plant Genetics and Crop Plant Research. Department of Physiology and Cell Biology. Metalloid Transport Group; Alemania Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Yaneff, Agustín. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina Fil: Barberini, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina Fil: Mecchia, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina Fil: Amodeo, Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina 2017-08-30T13:51:00Z 2017-08-30T13:51:00Z 2016 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:ar-repo/semantics/artículo http://hdl.handle.net/20.500.12123/1080 http://www.plantcell.org/content/plantcell/28/5/1053.full.pdf 1040-4651 (Print) 1532-298X (Online) https://doi.org/10.1105/tpc.15.00776 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Plant cell 28 (5) :1053-1077. (May 2016) |
| spellingShingle | Arabidopsis Thaliana Polinización Polen Pollination Pollen Perez Di Giorgio, Juliana Andrea Bienert, Gerd Patrick Ayub, Nicolás Daniel Yaneff, Agustín Barberini, María Laura Mecchia, Martín Alejandro Amodeo, Gabriela Soto, Gabriela Cinthia Muschietti, Jorge Prometeo Pollen-specific aquaporins NIP4;1 and NIP4;2 are required for pollen development and pollination in Arabidopsis thaliana |
| title | Pollen-specific aquaporins NIP4;1 and NIP4;2 are required for pollen development and pollination in Arabidopsis thaliana |
| title_full | Pollen-specific aquaporins NIP4;1 and NIP4;2 are required for pollen development and pollination in Arabidopsis thaliana |
| title_fullStr | Pollen-specific aquaporins NIP4;1 and NIP4;2 are required for pollen development and pollination in Arabidopsis thaliana |
| title_full_unstemmed | Pollen-specific aquaporins NIP4;1 and NIP4;2 are required for pollen development and pollination in Arabidopsis thaliana |
| title_short | Pollen-specific aquaporins NIP4;1 and NIP4;2 are required for pollen development and pollination in Arabidopsis thaliana |
| title_sort | pollen specific aquaporins nip4 1 and nip4 2 are required for pollen development and pollination in arabidopsis thaliana |
| topic | Arabidopsis Thaliana Polinización Polen Pollination Pollen |
| url | http://hdl.handle.net/20.500.12123/1080 http://www.plantcell.org/content/plantcell/28/5/1053.full.pdf https://doi.org/10.1105/tpc.15.00776 |
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