The chlorotic symptom induced by Sunflower chlorotic mottle virus is associated with changes in redox-related gene expression and metabolites
Systemic infections are commonly associated with changes in host metabolism and gene expression. Sunflower chlorotic mottle virus (SuCMoV) causes systemic infection with sugar increase, photoinhibition and increase in antioxidant enzyme activities before chlorotic symptom appearance in sunflower lea...
| Main Authors: | , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Elsevier
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/10790 https://www.sciencedirect.com/science/article/pii/S0168945212001665 https://doi.org/10.1016/j.plantsci.2012.08.008 |
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| author | Rodriguez, Marianela Muñoz, Nacira Belen Lenardon, Sergio Luis Lascano, Hernan Ramiro |
| author_browse | Lascano, Hernan Ramiro Lenardon, Sergio Luis Muñoz, Nacira Belen Rodriguez, Marianela |
| author_facet | Rodriguez, Marianela Muñoz, Nacira Belen Lenardon, Sergio Luis Lascano, Hernan Ramiro |
| author_sort | Rodriguez, Marianela |
| collection | INTA Digital |
| description | Systemic infections are commonly associated with changes in host metabolism and gene expression. Sunflower chlorotic mottle virus (SuCMoV) causes systemic infection with sugar increase, photoinhibition and increase in antioxidant enzyme activities before chlorotic symptom appearance in sunflower leaves. The aim of this study was to determine if chlorotic symptom development induced by SuCMoV infection is accompanied by changes in different redox-related metabolites and transcripts. Symptom development was analyzed in the second pair of leaves (systemic infection) at different post-inoculation times: before symptom appearance (BS, 4 dpi), and at an early (ES, 7 dpi) and later stage (LS, 12 dpi) of symptom expression. The results showed that the virus reaches the second pair of leaves at 4 dpi. A positive correlation between chlorotic symptom and number of viral copies was also observed. Changes in hydrogen peroxide, glutathione, pyridine nucleotides and ATP content were observed since symptom appearance (ES, 7 dpi). The expression of some of the genes analyzed was also strongly affected by SuCMoV infection. Specifically, down-regulation of both chloroplast-encoded genes and chloroplast-targeted genes: psbA, rbcS, Cu/Zn sod, Fe sod, phosphoglycolate phosphatase, psbO, psaH and fnr was present, whereas the expression of cytoplasmic-targeted genes, apx1, and Cu/Zn sod was up-regulated. Mitochondrial Mn sod decreased at BS stage and aox decreased only at ES stage. Peroxisomal catalase (cat-2) was lower at BS and LS stages. All these results suggest that SuCMoV infection induces progressive changes in determinants of redox homeostasis associated with chlorotic symptom development. |
| format | Artículo |
| id | INTA10790 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA107902021-11-29T12:50:23Z The chlorotic symptom induced by Sunflower chlorotic mottle virus is associated with changes in redox-related gene expression and metabolites Rodriguez, Marianela Muñoz, Nacira Belen Lenardon, Sergio Luis Lascano, Hernan Ramiro Gene Expression Plant Diseases Helianthus Annuus Expresión Génica Enfermedades de las Plantas Redox State Symptom Development Sunflower SuCMoV Girasol Systemic infections are commonly associated with changes in host metabolism and gene expression. Sunflower chlorotic mottle virus (SuCMoV) causes systemic infection with sugar increase, photoinhibition and increase in antioxidant enzyme activities before chlorotic symptom appearance in sunflower leaves. The aim of this study was to determine if chlorotic symptom development induced by SuCMoV infection is accompanied by changes in different redox-related metabolites and transcripts. Symptom development was analyzed in the second pair of leaves (systemic infection) at different post-inoculation times: before symptom appearance (BS, 4 dpi), and at an early (ES, 7 dpi) and later stage (LS, 12 dpi) of symptom expression. The results showed that the virus reaches the second pair of leaves at 4 dpi. A positive correlation between chlorotic symptom and number of viral copies was also observed. Changes in hydrogen peroxide, glutathione, pyridine nucleotides and ATP content were observed since symptom appearance (ES, 7 dpi). The expression of some of the genes analyzed was also strongly affected by SuCMoV infection. Specifically, down-regulation of both chloroplast-encoded genes and chloroplast-targeted genes: psbA, rbcS, Cu/Zn sod, Fe sod, phosphoglycolate phosphatase, psbO, psaH and fnr was present, whereas the expression of cytoplasmic-targeted genes, apx1, and Cu/Zn sod was up-regulated. Mitochondrial Mn sod decreased at BS stage and aox decreased only at ES stage. Peroxisomal catalase (cat-2) was lower at BS and LS stages. All these results suggest that SuCMoV infection induces progressive changes in determinants of redox homeostasis associated with chlorotic symptom development. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina 2021-11-29T12:44:52Z 2021-11-29T12:44:52Z 2012-08-18 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10790 https://www.sciencedirect.com/science/article/pii/S0168945212001665 0168-9452 https://doi.org/10.1016/j.plantsci.2012.08.008 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Plant Science 196 : 107-116 (November 2012) |
| spellingShingle | Gene Expression Plant Diseases Helianthus Annuus Expresión Génica Enfermedades de las Plantas Redox State Symptom Development Sunflower SuCMoV Girasol Rodriguez, Marianela Muñoz, Nacira Belen Lenardon, Sergio Luis Lascano, Hernan Ramiro The chlorotic symptom induced by Sunflower chlorotic mottle virus is associated with changes in redox-related gene expression and metabolites |
| title | The chlorotic symptom induced by Sunflower chlorotic mottle virus is associated with changes in redox-related gene expression and metabolites |
| title_full | The chlorotic symptom induced by Sunflower chlorotic mottle virus is associated with changes in redox-related gene expression and metabolites |
| title_fullStr | The chlorotic symptom induced by Sunflower chlorotic mottle virus is associated with changes in redox-related gene expression and metabolites |
| title_full_unstemmed | The chlorotic symptom induced by Sunflower chlorotic mottle virus is associated with changes in redox-related gene expression and metabolites |
| title_short | The chlorotic symptom induced by Sunflower chlorotic mottle virus is associated with changes in redox-related gene expression and metabolites |
| title_sort | chlorotic symptom induced by sunflower chlorotic mottle virus is associated with changes in redox related gene expression and metabolites |
| topic | Gene Expression Plant Diseases Helianthus Annuus Expresión Génica Enfermedades de las Plantas Redox State Symptom Development Sunflower SuCMoV Girasol |
| url | http://hdl.handle.net/20.500.12123/10790 https://www.sciencedirect.com/science/article/pii/S0168945212001665 https://doi.org/10.1016/j.plantsci.2012.08.008 |
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