Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis
Leptospirosis is the most widespread zoonosis worldwide, and it can cause reproductive failures in livestock, while in humans may vary from a mild fever to multi-organ failure and death. Due to this, in this study, we evaluated the usefulness of the segment encoding LigB C-terminus region, only pres...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/10776 https://www.sciencedirect.com/science/article/abs/pii/S0147957121000813 https://doi.org/10.1016/j.cimid.2021.101689 |
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| author | Saraullo, Vanina Rosa Grune Loffler, Sylvia Florin-Christensen, Mónica Watanabe, Olivia Hamer, Micaela Martinez, Mara Leila Brihuega, Bibiana Felicitas |
| author_browse | Brihuega, Bibiana Felicitas Florin-Christensen, Mónica Grune Loffler, Sylvia Hamer, Micaela Martinez, Mara Leila Saraullo, Vanina Rosa Watanabe, Olivia |
| author_facet | Saraullo, Vanina Rosa Grune Loffler, Sylvia Florin-Christensen, Mónica Watanabe, Olivia Hamer, Micaela Martinez, Mara Leila Brihuega, Bibiana Felicitas |
| author_sort | Saraullo, Vanina Rosa |
| collection | INTA Digital |
| description | Leptospirosis is the most widespread zoonosis worldwide, and it can cause reproductive failures in livestock, while in humans may vary from a mild fever to multi-organ failure and death. Due to this, in this study, we evaluated the usefulness of the segment encoding LigB C-terminus region, only present in pathogenic as target for a diagnostic PCR. This new PCR yielded a 100 % positivity for pathogenic Leptospira species and no cross-reactivity was found with intermediate or non-pathogenic species, or with other microorganisms, demostrating its high analytical specificity. The estimated analytical sensitivity was higher in serum samples than in blood or urine samples (6−9 × 102 lept/mL and 6−9 × 105 and 6−9 × 106 lept/mL, respectively). Multiple sequence alignment of the target region from different pathogenic Leptospira species confirmed that this gene region is highly conserved among these species, with few single nucleotide polymorphisms. The ligb-ct PCR here developed appears as a useful tool for the molecular diagnosis of leptospirosis. |
| format | Artículo |
| id | INTA10776 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA107762021-11-15T16:30:02Z Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis Saraullo, Vanina Rosa Grune Loffler, Sylvia Florin-Christensen, Mónica Watanabe, Olivia Hamer, Micaela Martinez, Mara Leila Brihuega, Bibiana Felicitas Leptospirosis Enfermedades de los Animales PCR Diagnóstico Genética Animal Diseases Diagnosis Genetics Leptospira Leptospirosis is the most widespread zoonosis worldwide, and it can cause reproductive failures in livestock, while in humans may vary from a mild fever to multi-organ failure and death. Due to this, in this study, we evaluated the usefulness of the segment encoding LigB C-terminus region, only present in pathogenic as target for a diagnostic PCR. This new PCR yielded a 100 % positivity for pathogenic Leptospira species and no cross-reactivity was found with intermediate or non-pathogenic species, or with other microorganisms, demostrating its high analytical specificity. The estimated analytical sensitivity was higher in serum samples than in blood or urine samples (6−9 × 102 lept/mL and 6−9 × 105 and 6−9 × 106 lept/mL, respectively). Multiple sequence alignment of the target region from different pathogenic Leptospira species confirmed that this gene region is highly conserved among these species, with few single nucleotide polymorphisms. The ligb-ct PCR here developed appears as a useful tool for the molecular diagnosis of leptospirosis. Instituto de Patobiología Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Fil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Jacobsen, Mónica Ofelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Fil: Jacobsen, Mónica Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Watanabe, Olivia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Fil: Watanabe, Olivia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-11-15T13:31:05Z 2021-11-15T13:31:05Z 2021-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10776 https://www.sciencedirect.com/science/article/abs/pii/S0147957121000813 0147-9571 https://doi.org/10.1016/j.cimid.2021.101689 eng info:eu-repograntAgreement/INTA/PNSA-1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional. info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Comparative Immunology, Microbiology and Infectious Diseases 78 : 101689 (October 2021) |
| spellingShingle | Leptospirosis Enfermedades de los Animales PCR Diagnóstico Genética Animal Diseases Diagnosis Genetics Leptospira Saraullo, Vanina Rosa Grune Loffler, Sylvia Florin-Christensen, Mónica Watanabe, Olivia Hamer, Micaela Martinez, Mara Leila Brihuega, Bibiana Felicitas Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis |
| title | Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis |
| title_full | Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis |
| title_fullStr | Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis |
| title_full_unstemmed | Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis |
| title_short | Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis |
| title_sort | use of the leptospira sp ligb c terminus coding region as a diagnostic tool of animal leptospirosis |
| topic | Leptospirosis Enfermedades de los Animales PCR Diagnóstico Genética Animal Diseases Diagnosis Genetics Leptospira |
| url | http://hdl.handle.net/20.500.12123/10776 https://www.sciencedirect.com/science/article/abs/pii/S0147957121000813 https://doi.org/10.1016/j.cimid.2021.101689 |
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