Imagen de Portada
Código QR

Rapid and cost-effective process based on insect larvae for scale-up production of SARS-COV-2 spike protein for serological COVID-19 testing

Serology testing for COVID-19 is important in evaluating active immune response against SARS-CoV-2, studying the antibody kinetics, and monitoring reinfections with genetic variants and new virus strains, in particular, the duration of antibodies in virus-exposed individuals and vaccine-mediated imm...

Descripción completa

Autores principales: Smith, Ignacio, Mc Callum, Gregorio Juan, Sabljic, Adriana Victoria, Marfia, Juan Ignacio, Bombicino, Silvina Sonia, Trabucchi, Aldana, Iacono, Ruben Francisco, Birenbaum, Joaquín Manuel, Vazquez, Susana Claudia, Minoia, Juan Mauricio, Cascone, Osvaldo, Lopez, Maria Gabriela, Taboga, Oscar Alberto, Targovnik, Alexandra Marisa, Wolman, Federico Javier, Fingermann, Matías, Alonso, Leonardo Gabriel, Valdez, Silvina Noemí, Miranda, María Victoria
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: Wiley 2021
Materias:
COVID-19
Coronavirus del Síndrome Respiratorio Agudo Grave 2
Ensayo
Larvas de Insectos
Proteínas
Severe Acute Respiratory Syndrome Coronavirus 2
Testing
Insect Larvae
Proteins
SARS-CoV-2
Acceso en línea:http://hdl.handle.net/20.500.12123/10759
https://onlinelibrary.wiley.com/doi/full/10.1002/bit.27889
https://doi.org/10.1002/bit.27889
Sumario:Serology testing for COVID-19 is important in evaluating active immune response against SARS-CoV-2, studying the antibody kinetics, and monitoring reinfections with genetic variants and new virus strains, in particular, the duration of antibodies in virus-exposed individuals and vaccine-mediated immunity. In this study, recombinant S protein of SARS-CoV-2 was expressed in Rachiplusia nu, an important agronomic plague. One gram of insect larvae produces an amount of S protein sufficient for 150 determinations in the ELISA method herein developed. We established a rapid production process for SARS-CoV-2 S protein that showed immunoreactivity for anti-SARS-CoV-2 antibodies and was used as a single antigen for developing the ELISA method with high sensitivity (96.2%) and specificity (98.8%). Our findings provide an efficient and cost-effective platform for large-scale S protein production, and the scale-up is linear, thus avoiding the use of complex equipment like bioreactors.

Ejemplares similares