Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristics
Adult male Llamas (Lama glama) (n = 6) were collected using a receptive female and a thermo-electric artificial vagina assembled with a polyethylene collection bag with the following objectives: (a) to develop a reliable and repeatable semen collection technique for Llama, (b) validate semen evaluat...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/10753 https://www.sciencedirect.com/science/article/abs/pii/S0921448815300523 https://doi.org/10.1016/j.smallrumres.2015.08.016 |
| _version_ | 1855036447219777536 |
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| author | Ferre, Luis Bernardo Malik, Genoveva Aller Atucha, Juan Florencio Alberio, Ricardo Fresno, Cristóbal Kjelland, Michael |
| author_browse | Alberio, Ricardo Aller Atucha, Juan Florencio Ferre, Luis Bernardo Fresno, Cristóbal Kjelland, Michael Malik, Genoveva |
| author_facet | Ferre, Luis Bernardo Malik, Genoveva Aller Atucha, Juan Florencio Alberio, Ricardo Fresno, Cristóbal Kjelland, Michael |
| author_sort | Ferre, Luis Bernardo |
| collection | INTA Digital |
| description | Adult male Llamas (Lama glama) (n = 6) were collected using a receptive female and a thermo-electric artificial vagina assembled with a polyethylene collection bag with the following objectives: (a) to develop a reliable and repeatable semen collection technique for Llama, (b) validate semen evaluation tests for Llama, and (c) to determine the effect of collection frequency and season on Llama semen characteristics. First, the semen and sperm variables were recorded and validated through their own repeatability. Semen collection intervals tested were: 1X/week for three weeks and 3X/week for another three weeks, the second collection period occurring after two weeks of sexual rest. The collection frequency of 3X/week significantly reduced (P < 0.05) the sperm viability, motility, concentration, Total Sperm/Ejaculate, Total Motile Sperm/Ejaculate and Total Live Sperm/Ejaculate, but improved Total Sperm/Week and Total Live Sperm/Week. All recorded variables were significantly higher during the summer in comparison to the spring with the exception of morphology abnormalities, volume, and viability. Also, the 1X/week versus the 3X/week semen collection frequencies did not produce a significant difference in the percent of total motile sperm/week. Based on semen and sperm characteristics evaluated herein, Llama semen collected in the summer was better (P < 0.05), with regard to the majority of the variables analyzed, than semen collected in the spring. |
| format | info:ar-repo/semantics/artículo |
| id | INTA10753 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA107532021-11-11T17:23:34Z Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristics Ferre, Luis Bernardo Malik, Genoveva Aller Atucha, Juan Florencio Alberio, Ricardo Fresno, Cristóbal Kjelland, Michael Llama Eyaculación Recolección de Semen Evaluación Espermatozoo Estacionalidad Llamas Ejaculation Semen Collection Evaluation Spermatozoa Seasonality Adult male Llamas (Lama glama) (n = 6) were collected using a receptive female and a thermo-electric artificial vagina assembled with a polyethylene collection bag with the following objectives: (a) to develop a reliable and repeatable semen collection technique for Llama, (b) validate semen evaluation tests for Llama, and (c) to determine the effect of collection frequency and season on Llama semen characteristics. First, the semen and sperm variables were recorded and validated through their own repeatability. Semen collection intervals tested were: 1X/week for three weeks and 3X/week for another three weeks, the second collection period occurring after two weeks of sexual rest. The collection frequency of 3X/week significantly reduced (P < 0.05) the sperm viability, motility, concentration, Total Sperm/Ejaculate, Total Motile Sperm/Ejaculate and Total Live Sperm/Ejaculate, but improved Total Sperm/Week and Total Live Sperm/Week. All recorded variables were significantly higher during the summer in comparison to the spring with the exception of morphology abnormalities, volume, and viability. Also, the 1X/week versus the 3X/week semen collection frequencies did not produce a significant difference in the percent of total motile sperm/week. Based on semen and sperm characteristics evaluated herein, Llama semen collected in the summer was better (P < 0.05), with regard to the majority of the variables analyzed, than semen collected in the spring. EEA Balcarce Fil: Ferré Luis Bernardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Malik, Genoveva. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Alberio, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Fresno, Cristóbal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Córdoba. Facultad de Ingeniería; Argentina. Fil: Kjelland, Michael. Conservation, Genetics & Biotech; Estados Unidos. 2021-11-11T17:18:25Z 2021-11-11T17:18:25Z 2015-09-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10753 https://www.sciencedirect.com/science/article/abs/pii/S0921448815300523 0921-4488 https://doi.org/10.1016/j.smallrumres.2015.08.016 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Small Ruminant Research 133 : 140-147 (december 2015) |
| spellingShingle | Llama Eyaculación Recolección de Semen Evaluación Espermatozoo Estacionalidad Llamas Ejaculation Semen Collection Evaluation Spermatozoa Seasonality Ferre, Luis Bernardo Malik, Genoveva Aller Atucha, Juan Florencio Alberio, Ricardo Fresno, Cristóbal Kjelland, Michael Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristics |
| title | Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristics |
| title_full | Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristics |
| title_fullStr | Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristics |
| title_full_unstemmed | Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristics |
| title_short | Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristics |
| title_sort | llama lama glama semen collection via thermo electric artificial vagina effect of seasonality and collection interval on ejaculate characteristics |
| topic | Llama Eyaculación Recolección de Semen Evaluación Espermatozoo Estacionalidad Llamas Ejaculation Semen Collection Evaluation Spermatozoa Seasonality |
| url | http://hdl.handle.net/20.500.12123/10753 https://www.sciencedirect.com/science/article/abs/pii/S0921448815300523 https://doi.org/10.1016/j.smallrumres.2015.08.016 |
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