Functional characterization and intracellular localization of a Lotus tenuis NHX antiporter
Lotus tenuis is a perennial forage legume widely scattered in the flooding Pampa region of Argentina. Even when it is considered a salt-tolerant species, the molecular basis underlying this trait still remains unknown. Intracellular compartmentation of Na+ into vacuoles by NHX antiporters is an impo...
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/10747 https://link.springer.com/article/10.1007%2Fs11240-021-02094-3 https://doi.org/10.1007/s11240-021-02094-3 |
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| author | Affinito, María Agostina Espasandin, Fabiana Daniela Del Vas, Mariana Alvarez, Mayra Yanet Andres, Adriana Noemi Sansberro, Pedro Alfonso Diaz Paleo, Antonio Horacio |
| author_browse | Affinito, María Agostina Alvarez, Mayra Yanet Andres, Adriana Noemi Del Vas, Mariana Diaz Paleo, Antonio Horacio Espasandin, Fabiana Daniela Sansberro, Pedro Alfonso |
| author_facet | Affinito, María Agostina Espasandin, Fabiana Daniela Del Vas, Mariana Alvarez, Mayra Yanet Andres, Adriana Noemi Sansberro, Pedro Alfonso Diaz Paleo, Antonio Horacio |
| author_sort | Affinito, María Agostina |
| collection | INTA Digital |
| description | Lotus tenuis is a perennial forage legume widely scattered in the flooding Pampa region of Argentina. Even when it is considered a salt-tolerant species, the molecular basis underlying this trait still remains unknown. Intracellular compartmentation of Na+ into vacuoles by NHX antiporters is an important and well known strategy to reduce ion toxicity during salt stress. In this study, the subcellular localization of Lotus tenuis NHX1 was determined by the transient expression of a LtNHX1-GFP fusion protein in Nicotiana benthamiana agroinfiltrated leaves, confirming that it is a tonoplast-located NHX antiporter. To study LtNHX1 contribution to salinity tolerance, a transgenic Lotus tenuis line was obtained by Agrobacterium-mediated transformation. Overexpression of the transgene was confirmed by PCR and RT-PCR analyses. Compared to wild-type, transgenic plants displayed less sensitivity and grew better under high salt stress, with higher survival rates, shoot length, and dry weight. These results suggest that the activity of the tonoplast-located LtNHX1 antiporter contributes to Lotus tenuis salt tolerance. |
| format | Artículo |
| id | INTA10747 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA107472021-11-11T12:32:01Z Functional characterization and intracellular localization of a Lotus tenuis NHX antiporter Affinito, María Agostina Espasandin, Fabiana Daniela Del Vas, Mariana Alvarez, Mayra Yanet Andres, Adriana Noemi Sansberro, Pedro Alfonso Diaz Paleo, Antonio Horacio Lotus tenuis Estrés Osmótico Plantas Transgénicas Genética Osmotic Stress Transgenic Plants Genetics Estrés Salino Lotus tenuis is a perennial forage legume widely scattered in the flooding Pampa region of Argentina. Even when it is considered a salt-tolerant species, the molecular basis underlying this trait still remains unknown. Intracellular compartmentation of Na+ into vacuoles by NHX antiporters is an important and well known strategy to reduce ion toxicity during salt stress. In this study, the subcellular localization of Lotus tenuis NHX1 was determined by the transient expression of a LtNHX1-GFP fusion protein in Nicotiana benthamiana agroinfiltrated leaves, confirming that it is a tonoplast-located NHX antiporter. To study LtNHX1 contribution to salinity tolerance, a transgenic Lotus tenuis line was obtained by Agrobacterium-mediated transformation. Overexpression of the transgene was confirmed by PCR and RT-PCR analyses. Compared to wild-type, transgenic plants displayed less sensitivity and grew better under high salt stress, with higher survival rates, shoot length, and dry weight. These results suggest that the activity of the tonoplast-located LtNHX1 antiporter contributes to Lotus tenuis salt tolerance. Instituto de Biotecnología Fil: Affinito, María Agostina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina Fil: Affinito, María Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina Fil: Affinito, María Agostina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y Ambientales; Argentina Fil: Espasandin, Fabiana Daniela. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste; Argentina Fil: Espasandin, Fabiana Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Alvarez, Mayra Yanet. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste; Argentina Fil: Alvarez, Mayra Yanet. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina Fil: Andrés, Adriana Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Forrajeras; Argentina. Fil: Andrés, Adriana Noemi. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y Ambientales; Argentina Fil: Sansberro, Pedro Alfonso. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste; Argentina Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina Fil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina. Fil: Diaz Paleo, Antonio Horacio. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y Ambientales; Argentina 2021-11-11T12:27:31Z 2021-11-11T12:27:31Z 2021-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10747 https://link.springer.com/article/10.1007%2Fs11240-021-02094-3 0167-6857 1573-5044 https://doi.org/10.1007/s11240-021-02094-3 eng info:eu-repograntAgreement/INTA/PNPA-1126072/AR./Desarrollo de cultivares superiores de especies forrajeras para sistemas ganaderos y agricolo-ganaderos de la Argentina. info:eu-repo/semantics/restrictedAccess application/pdf Springer Plant Cell, Tissue and Organ Culture (PCTOC) 146 : 565–576 (2021) |
| spellingShingle | Lotus tenuis Estrés Osmótico Plantas Transgénicas Genética Osmotic Stress Transgenic Plants Genetics Estrés Salino Affinito, María Agostina Espasandin, Fabiana Daniela Del Vas, Mariana Alvarez, Mayra Yanet Andres, Adriana Noemi Sansberro, Pedro Alfonso Diaz Paleo, Antonio Horacio Functional characterization and intracellular localization of a Lotus tenuis NHX antiporter |
| title | Functional characterization and intracellular localization of a Lotus tenuis NHX antiporter |
| title_full | Functional characterization and intracellular localization of a Lotus tenuis NHX antiporter |
| title_fullStr | Functional characterization and intracellular localization of a Lotus tenuis NHX antiporter |
| title_full_unstemmed | Functional characterization and intracellular localization of a Lotus tenuis NHX antiporter |
| title_short | Functional characterization and intracellular localization of a Lotus tenuis NHX antiporter |
| title_sort | functional characterization and intracellular localization of a lotus tenuis nhx antiporter |
| topic | Lotus tenuis Estrés Osmótico Plantas Transgénicas Genética Osmotic Stress Transgenic Plants Genetics Estrés Salino |
| url | http://hdl.handle.net/20.500.12123/10747 https://link.springer.com/article/10.1007%2Fs11240-021-02094-3 https://doi.org/10.1007/s11240-021-02094-3 |
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