Comparative study on the in vitro replication and genomic variability of Argentinean field isolates of bovine herpesvirus type 4 (BoHV-4)
Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus, belonging to the Rhadinovirus genus, which is increasingly associated with various problems of the reproductive tract of cattle. In Argentina, analysis of BoHV-4 strains isolated from cervico-vaginal mucus of aborted cows revealed a high genetic d...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1073 https://link.springer.com/article/10.1007%2Fs11262-016-1312-3 https://doi.org/10.1007/s11262-016-1312-3 |
| _version_ | 1855034743292166144 |
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| author | Verna, Andrea Elizabeth Perez, Sandra Manrique, Julieta Marina Leunda, Maria Rosa Odeon, Anselmo Carlos Jones, Leandro Roberto |
| author_browse | Jones, Leandro Roberto Leunda, Maria Rosa Manrique, Julieta Marina Odeon, Anselmo Carlos Perez, Sandra Verna, Andrea Elizabeth |
| author_facet | Verna, Andrea Elizabeth Perez, Sandra Manrique, Julieta Marina Leunda, Maria Rosa Odeon, Anselmo Carlos Jones, Leandro Roberto |
| author_sort | Verna, Andrea Elizabeth |
| collection | INTA Digital |
| description | Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus, belonging to the Rhadinovirus genus, which is increasingly associated with various problems of the reproductive tract of cattle. In Argentina, analysis of BoHV-4 strains isolated from cervico-vaginal mucus of aborted cows revealed a high genetic divergence among strains, which could be classified in three different groups: Genotype 1 comprises Movar-like strains (European prototype), Genotype 2 includes DN599 like strains (American prototype) and Genotype 3 corresponds to a novel genotype group. Understanding the replication behavior in cell cultures and the molecular characteristics of this pathogen of cattle is critical for the rational design of in vitro experiments. The aim of this work was to quantitatively evaluate the replication properties of different Argentinean BoHV-4 strains and to characterize their phylogenetic relationships. Significant differences were evident among the virus titers of the different BoHV-4 isolates in vitro. The most conserved gene was the major capsid protein (ORF25). The glycoprotein B (gB), glycoprotein H (gH), and thymidine kinsase (TK) genes displayed both synonymous and nonsynonymous substitutions, with the highest diversity observed for gB, which displayed amino acid substitutions in 24 out of the 178 positions examined. Strains 09/759, 12/512, and 07/568 presented a deletion encompassing amino acid position 27 to 35, whereas strains 07/435 and 09/227 had a deletion from position 28 to 35. Two strains, 07/435 and 09/227, also displayed the highest divergence compared to the other strains analyzed. This study provides information about the in vitro replication and behavior of nine field isolates of BoHV-4. These findings are relevant since available information on the in vitro growth characteristics of BoHV-4 strains is scarce. The results from this study may also be useful for establishing comparisons with other related viruses. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1073 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA10732019-06-13T15:36:17Z Comparative study on the in vitro replication and genomic variability of Argentinean field isolates of bovine herpesvirus type 4 (BoHV-4) Verna, Andrea Elizabeth Perez, Sandra Manrique, Julieta Marina Leunda, Maria Rosa Odeon, Anselmo Carlos Jones, Leandro Roberto Enfermedades de los Animales Herpes Virus Bovino Variación Genética Experimentación in Vitro Animal Diseases Bovine Herpesvirus Genetic Variation In Vitro Experimentation Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus, belonging to the Rhadinovirus genus, which is increasingly associated with various problems of the reproductive tract of cattle. In Argentina, analysis of BoHV-4 strains isolated from cervico-vaginal mucus of aborted cows revealed a high genetic divergence among strains, which could be classified in three different groups: Genotype 1 comprises Movar-like strains (European prototype), Genotype 2 includes DN599 like strains (American prototype) and Genotype 3 corresponds to a novel genotype group. Understanding the replication behavior in cell cultures and the molecular characteristics of this pathogen of cattle is critical for the rational design of in vitro experiments. The aim of this work was to quantitatively evaluate the replication properties of different Argentinean BoHV-4 strains and to characterize their phylogenetic relationships. Significant differences were evident among the virus titers of the different BoHV-4 isolates in vitro. The most conserved gene was the major capsid protein (ORF25). The glycoprotein B (gB), glycoprotein H (gH), and thymidine kinsase (TK) genes displayed both synonymous and nonsynonymous substitutions, with the highest diversity observed for gB, which displayed amino acid substitutions in 24 out of the 178 positions examined. Strains 09/759, 12/512, and 07/568 presented a deletion encompassing amino acid position 27 to 35, whereas strains 07/435 and 09/227 had a deletion from position 28 to 35. Two strains, 07/435 and 09/227, also displayed the highest divergence compared to the other strains analyzed. This study provides information about the in vitro replication and behavior of nine field isolates of BoHV-4. These findings are relevant since available information on the in vitro growth characteristics of BoHV-4 strains is scarce. The results from this study may also be useful for establishing comparisons with other related viruses. Inst.de Virología Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Perez, Sandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina Fil: Manrique, Julieta Marina. Universidad Nacional de la Patagonia San Juan Bosco. Facultad de Ciencias Naturales - Sede Trelew. Laboratorio de Virología y Genética Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Jones, Leandro Roberto. Universidad Nacional de la Patagonia San Juan Bosco. Facultad de Ciencias Naturales - Sede Trelew. Laboratorio de Virología y Genética Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-08-29T17:35:13Z 2017-08-29T17:35:13Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1073 https://link.springer.com/article/10.1007%2Fs11262-016-1312-3 0920-8569 (Print) 1572-994X (Online) https://doi.org/10.1007/s11262-016-1312-3 eng info:eu-repo/semantics/restrictedAccess application/pdf Argentina (nation) Virus genes 52 (3) : 372–378. (June 2016) |
| spellingShingle | Enfermedades de los Animales Herpes Virus Bovino Variación Genética Experimentación in Vitro Animal Diseases Bovine Herpesvirus Genetic Variation In Vitro Experimentation Verna, Andrea Elizabeth Perez, Sandra Manrique, Julieta Marina Leunda, Maria Rosa Odeon, Anselmo Carlos Jones, Leandro Roberto Comparative study on the in vitro replication and genomic variability of Argentinean field isolates of bovine herpesvirus type 4 (BoHV-4) |
| title | Comparative study on the in vitro replication and genomic variability of Argentinean field isolates of bovine herpesvirus type 4 (BoHV-4) |
| title_full | Comparative study on the in vitro replication and genomic variability of Argentinean field isolates of bovine herpesvirus type 4 (BoHV-4) |
| title_fullStr | Comparative study on the in vitro replication and genomic variability of Argentinean field isolates of bovine herpesvirus type 4 (BoHV-4) |
| title_full_unstemmed | Comparative study on the in vitro replication and genomic variability of Argentinean field isolates of bovine herpesvirus type 4 (BoHV-4) |
| title_short | Comparative study on the in vitro replication and genomic variability of Argentinean field isolates of bovine herpesvirus type 4 (BoHV-4) |
| title_sort | comparative study on the in vitro replication and genomic variability of argentinean field isolates of bovine herpesvirus type 4 bohv 4 |
| topic | Enfermedades de los Animales Herpes Virus Bovino Variación Genética Experimentación in Vitro Animal Diseases Bovine Herpesvirus Genetic Variation In Vitro Experimentation |
| url | http://hdl.handle.net/20.500.12123/1073 https://link.springer.com/article/10.1007%2Fs11262-016-1312-3 https://doi.org/10.1007/s11262-016-1312-3 |
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