Neospora caninum and Toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from Argentina
The aim of this study is to provide preliminary data about the occurrence of N. caninum- and T. gondii-related abortions and perinatal deaths in sheep from Argentina. Thirty ovine aborted foetuses and 33 perinatal deaths were submitted to the Veterinary Diagnostic Service at INTA EEA Balcarce (Argen...
| Main Authors: | , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/10650 https://www.sciencedirect.com/science/article/pii/S2667114X21000510 https://doi.org/10.1016/j.crpvbd.2021.100057 |
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| author | Della Rosa, Paola Fiorentino, María Andrea Morrell, Eleonora Lidia Scioli, Maria Valeria Paolicchi, Fernando Moore, Prando Dadin Canton, German Jose Hecker, Yanina |
| author_browse | Canton, German Jose Della Rosa, Paola Fiorentino, María Andrea Hecker, Yanina Moore, Prando Dadin Morrell, Eleonora Lidia Paolicchi, Fernando Scioli, Maria Valeria |
| author_facet | Della Rosa, Paola Fiorentino, María Andrea Morrell, Eleonora Lidia Scioli, Maria Valeria Paolicchi, Fernando Moore, Prando Dadin Canton, German Jose Hecker, Yanina |
| author_sort | Della Rosa, Paola |
| collection | INTA Digital |
| description | The aim of this study is to provide preliminary data about the occurrence of N. caninum- and T. gondii-related abortions and perinatal deaths in sheep from Argentina. Thirty ovine aborted foetuses and 33 perinatal deaths were submitted to the Veterinary Diagnostic Service at INTA EEA Balcarce (Argentina) during 2017–2019. A complete necropsy was performed on all specimens submitted, and foetal and placental tissues were examined. Foetal cavity fluids were collected for assessment of antibodies to N. caninum and T. gondii by indirect fluorescent antibody technique (IFAT). Placental and foetal tissue samples were collected for DNA extraction and histopathological analysis. The differential diagnosis with other causes of abortion was carried out. Of the sampled specimens, 20.63% (13/63) displayed evidence for N. caninum infection by IFAT and PCR, and in 61.5% (8/13) of the positive specimens the parasite was confirmed as the cause of abortion/perinatal death based on the presence of compatible histological lesions and/or positive immunohistochemistry test, positive PCR and/or positive IFAT, and no other infectious agents diagnosed. In contrast, T. gondii infection was confirmed in 9.52% (6/63) of the analysed specimens, but only in 2 lambs T. gondii was determined as the death cause. Neospora caninum and T. gondii co-infections were confirmed in 4 analysed specimens (2 aborted foetuses and 2 perinatal deaths). These results demonstrated that N. caninum is efficiently transmitted and a frequent cause of ovine reproductive failure in the commercial analysed flocks compared with T. gondii. Despite T. gondii congenital infection was detected in some specimens (6/63), it was confirmed as the cause of death in only two of them. Thus, and considering the limited availability of confirmed samples, we could not determine whether toxoplasmosis is a major problem in Argentinian sheep flocks or not. More studies on a greater number of specimens from different ovine production systems under different management conditions are necessary to assess the real impact of neosporosis and toxoplasmosis in Argentina. |
| format | Artículo |
| id | INTA10650 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA106502021-11-02T14:25:07Z Neospora caninum and Toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from Argentina Della Rosa, Paola Fiorentino, María Andrea Morrell, Eleonora Lidia Scioli, Maria Valeria Paolicchi, Fernando Moore, Prando Dadin Canton, German Jose Hecker, Yanina Ovinos Rebaños Enfermedades de los Animales Neospora Caninum Toxoplasma gondii Reproducción Mortalidad Perinatal Sheep Flocks Animal Diseases Reproduction Perinatal Mortality Argentina The aim of this study is to provide preliminary data about the occurrence of N. caninum- and T. gondii-related abortions and perinatal deaths in sheep from Argentina. Thirty ovine aborted foetuses and 33 perinatal deaths were submitted to the Veterinary Diagnostic Service at INTA EEA Balcarce (Argentina) during 2017–2019. A complete necropsy was performed on all specimens submitted, and foetal and placental tissues were examined. Foetal cavity fluids were collected for assessment of antibodies to N. caninum and T. gondii by indirect fluorescent antibody technique (IFAT). Placental and foetal tissue samples were collected for DNA extraction and histopathological analysis. The differential diagnosis with other causes of abortion was carried out. Of the sampled specimens, 20.63% (13/63) displayed evidence for N. caninum infection by IFAT and PCR, and in 61.5% (8/13) of the positive specimens the parasite was confirmed as the cause of abortion/perinatal death based on the presence of compatible histological lesions and/or positive immunohistochemistry test, positive PCR and/or positive IFAT, and no other infectious agents diagnosed. In contrast, T. gondii infection was confirmed in 9.52% (6/63) of the analysed specimens, but only in 2 lambs T. gondii was determined as the death cause. Neospora caninum and T. gondii co-infections were confirmed in 4 analysed specimens (2 aborted foetuses and 2 perinatal deaths). These results demonstrated that N. caninum is efficiently transmitted and a frequent cause of ovine reproductive failure in the commercial analysed flocks compared with T. gondii. Despite T. gondii congenital infection was detected in some specimens (6/63), it was confirmed as the cause of death in only two of them. Thus, and considering the limited availability of confirmed samples, we could not determine whether toxoplasmosis is a major problem in Argentinian sheep flocks or not. More studies on a greater number of specimens from different ovine production systems under different management conditions are necessary to assess the real impact of neosporosis and toxoplasmosis in Argentina. EEA Mercedes Fil: Della Rosa, Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Fiorentino, María Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Bacteriología; Argentina Fil: Morell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Patología; Argentina. Fil: Scioli, María Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Paoliochi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Bacteriología; Argentina Fil: Moore, Dadín. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. IPADS Balcarce. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Cantón, Germán Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Hercker, Yanina. IPADS Balcarce. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. 2021-11-02T13:48:48Z 2021-11-02T13:48:48Z 2021-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/10650 https://www.sciencedirect.com/science/article/pii/S2667114X21000510 2667-114X https://doi.org/10.1016/j.crpvbd.2021.100057 eng info:eu-repograntAgreement/INTA/PNSA-1115055/AR./Enfermedades infecciosas, parasitarias y toxico metabólicas que afectan la productividad de los ovinos, caprinos y camélidos. info:eu-repograntAgreement/INTA/2019-PE-E1-I002-001/2019-PE-E1-I002-001/AR./Tecnologías sostenibles para la estabilización y mejora de la competitividad de las cadenas productivas de ovinos, caprinos y camélidos sudamericanos. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Current Research in Parasitology & Vector-Borne Diseases : 100057 (Available online 1 November 2021) |
| spellingShingle | Ovinos Rebaños Enfermedades de los Animales Neospora Caninum Toxoplasma gondii Reproducción Mortalidad Perinatal Sheep Flocks Animal Diseases Reproduction Perinatal Mortality Argentina Della Rosa, Paola Fiorentino, María Andrea Morrell, Eleonora Lidia Scioli, Maria Valeria Paolicchi, Fernando Moore, Prando Dadin Canton, German Jose Hecker, Yanina Neospora caninum and Toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from Argentina |
| title | Neospora caninum and Toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from Argentina |
| title_full | Neospora caninum and Toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from Argentina |
| title_fullStr | Neospora caninum and Toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from Argentina |
| title_full_unstemmed | Neospora caninum and Toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from Argentina |
| title_short | Neospora caninum and Toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from Argentina |
| title_sort | neospora caninum and toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from argentina |
| topic | Ovinos Rebaños Enfermedades de los Animales Neospora Caninum Toxoplasma gondii Reproducción Mortalidad Perinatal Sheep Flocks Animal Diseases Reproduction Perinatal Mortality Argentina |
| url | http://hdl.handle.net/20.500.12123/10650 https://www.sciencedirect.com/science/article/pii/S2667114X21000510 https://doi.org/10.1016/j.crpvbd.2021.100057 |
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