Transcriptome profiling of Diachasmimorpha longicaudata towards useful molecular tools for population management
Background: Diachasmimorpha longicaudata (Hymenoptera: Braconidae) is a solitary parasitoid of Tephritidae (Diptera) fruit flies of economic importance currently being mass-reared in bio-factories and successfully used worldwide. A peculiar biological aspect of Hymenoptera is its haplo-diploid life...
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| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1060 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-016-2759-2 https://doi.org/10.1186/s12864-016-2759-2 |
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| author | Mannino, María Constanza Rivarola, Maximo Lisandro Scannapieco, Alejandra Carla Gonzalez, Sergio Alberto Farber, Marisa Diana Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz |
| author_browse | Cladera, Jorge Luis Farber, Marisa Diana Gonzalez, Sergio Alberto Lanzavecchia, Silvia Beatriz Mannino, María Constanza Rivarola, Maximo Lisandro Scannapieco, Alejandra Carla |
| author_facet | Mannino, María Constanza Rivarola, Maximo Lisandro Scannapieco, Alejandra Carla Gonzalez, Sergio Alberto Farber, Marisa Diana Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz |
| author_sort | Mannino, María Constanza |
| collection | INTA Digital |
| description | Background: Diachasmimorpha longicaudata (Hymenoptera: Braconidae) is a solitary parasitoid of Tephritidae (Diptera) fruit flies of economic importance currently being mass-reared in bio-factories and successfully used worldwide. A peculiar biological aspect of Hymenoptera is its haplo-diploid life cycle, where females (diploid) develop from fertilized eggs and males (haploid) from unfertilized eggs. Diploid males were described in many species and recently evidenced in D. longicaudata by mean of inbreeding studies. Sex determination in this parasitoid is based on the Complementary Sex Determination (CSD) system, with alleles from at least one locus involved in early steps of this pathway. Since limited information is available about genetics of this parasitoid
species, a deeper analysis on D. longicaudata’s genomics is required to provide molecular tools for achieving a more cost effective production under artificial rearing conditions.
Results: We report here the first transcriptome analysis of male-larvae, adult females and adult males of D. longicaudata using 454-pyrosequencing. A total of 469766 reads were analyzed and 8483 high-quality isotigs were assembled. After functional annotation, a total of 51686 unigenes were produced, from which, 7021 isotigs and 20227 singletons had at least one BLAST hit against the NCBI non-redundant protein database. A preliminary comparison of adult female and male evidenced that 98 transcripts showed differential expression profiles, with at
least a 10-fold difference. Among the functionally annotated transcripts we detected four sequences potentially involved in sex determination and three homologues to two known genes involved in the sex determination cascade. Finally, a total of 4674SimpleSequence Repeats (SSRs) were in silico identified and characterized. |
| format | info:eu-repo/semantics/article |
| id | INTA1060 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA10602018-01-08T12:20:49Z Transcriptome profiling of Diachasmimorpha longicaudata towards useful molecular tools for population management Mannino, María Constanza Rivarola, Maximo Lisandro Scannapieco, Alejandra Carla Gonzalez, Sergio Alberto Farber, Marisa Diana Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz Biosteres Longicaudatus Determinación del Sexo Expresión Génica Marcadores Genéticos Sex Determination Gene Expression Genetic Markers Diachasmimorpha Longicaudata Marcadores Moleculares Background: Diachasmimorpha longicaudata (Hymenoptera: Braconidae) is a solitary parasitoid of Tephritidae (Diptera) fruit flies of economic importance currently being mass-reared in bio-factories and successfully used worldwide. A peculiar biological aspect of Hymenoptera is its haplo-diploid life cycle, where females (diploid) develop from fertilized eggs and males (haploid) from unfertilized eggs. Diploid males were described in many species and recently evidenced in D. longicaudata by mean of inbreeding studies. Sex determination in this parasitoid is based on the Complementary Sex Determination (CSD) system, with alleles from at least one locus involved in early steps of this pathway. Since limited information is available about genetics of this parasitoid species, a deeper analysis on D. longicaudata’s genomics is required to provide molecular tools for achieving a more cost effective production under artificial rearing conditions. Results: We report here the first transcriptome analysis of male-larvae, adult females and adult males of D. longicaudata using 454-pyrosequencing. A total of 469766 reads were analyzed and 8483 high-quality isotigs were assembled. After functional annotation, a total of 51686 unigenes were produced, from which, 7021 isotigs and 20227 singletons had at least one BLAST hit against the NCBI non-redundant protein database. A preliminary comparison of adult female and male evidenced that 98 transcripts showed differential expression profiles, with at least a 10-fold difference. Among the functionally annotated transcripts we detected four sequences potentially involved in sex determination and three homologues to two known genes involved in the sex determination cascade. Finally, a total of 4674SimpleSequence Repeats (SSRs) were in silico identified and characterized. Inst. de Genética "Ewald A. Favret"- IGEAF Fil: Mannino, María Constanza. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina 2017-08-28T18:02:04Z 2017-08-28T18:02:04Z 2016 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1060 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-016-2759-2 1471-2164 https://doi.org/10.1186/s12864-016-2759-2 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BMC Genomics 17:793 (2016) |
| spellingShingle | Biosteres Longicaudatus Determinación del Sexo Expresión Génica Marcadores Genéticos Sex Determination Gene Expression Genetic Markers Diachasmimorpha Longicaudata Marcadores Moleculares Mannino, María Constanza Rivarola, Maximo Lisandro Scannapieco, Alejandra Carla Gonzalez, Sergio Alberto Farber, Marisa Diana Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz Transcriptome profiling of Diachasmimorpha longicaudata towards useful molecular tools for population management |
| title | Transcriptome profiling of Diachasmimorpha longicaudata towards useful molecular tools for population management |
| title_full | Transcriptome profiling of Diachasmimorpha longicaudata towards useful molecular tools for population management |
| title_fullStr | Transcriptome profiling of Diachasmimorpha longicaudata towards useful molecular tools for population management |
| title_full_unstemmed | Transcriptome profiling of Diachasmimorpha longicaudata towards useful molecular tools for population management |
| title_short | Transcriptome profiling of Diachasmimorpha longicaudata towards useful molecular tools for population management |
| title_sort | transcriptome profiling of diachasmimorpha longicaudata towards useful molecular tools for population management |
| topic | Biosteres Longicaudatus Determinación del Sexo Expresión Génica Marcadores Genéticos Sex Determination Gene Expression Genetic Markers Diachasmimorpha Longicaudata Marcadores Moleculares |
| url | http://hdl.handle.net/20.500.12123/1060 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-016-2759-2 https://doi.org/10.1186/s12864-016-2759-2 |
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