Hybridization and polyploidization effects on LTR‑retrotransposon activation in potato genome
Hybridization and polyploidization are major forces in plant evolution and potatoes are not an exception. It is proposed that the proliferation of Long Terminal Repeat-retrotransposons (LTR-RT) is related to genome reorganization caused by hybridization and/or polyploidization. The main purpose of...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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The Botanical Society of Japan
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/10592 https://link.springer.com/article/10.1007%2Fs10265-021-01354-9 https://doi.org/10.1007/s10265-021-01354-9 |
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| author | Gantuz, Magdalena Morales Sanfurgo, Hugo Andres Bertoldi, Maria Victoria Ibañez, Verónica Noé Duarte, Paola Fernanda Marfil, Carlos Federico Masuelli, Ricardo Williams |
| author_browse | Bertoldi, Maria Victoria Duarte, Paola Fernanda Gantuz, Magdalena Ibañez, Verónica Noé Marfil, Carlos Federico Masuelli, Ricardo Williams Morales Sanfurgo, Hugo Andres |
| author_facet | Gantuz, Magdalena Morales Sanfurgo, Hugo Andres Bertoldi, Maria Victoria Ibañez, Verónica Noé Duarte, Paola Fernanda Marfil, Carlos Federico Masuelli, Ricardo Williams |
| author_sort | Gantuz, Magdalena |
| collection | INTA Digital |
| description | Hybridization and polyploidization are major forces in plant evolution and potatoes are not an exception. It is proposed that the proliferation of Long Terminal Repeat-retrotransposons (LTR-RT) is related to genome reorganization caused by hybridization
and/or polyploidization. The main purpose of the present work was to evaluate the effect of interspecific hybridization and polyploidization on the activation of LTR-RT. We evaluated the proliferation of putative active LTR-RT in a diploid hybrid between the cultivated potato Solanum tuberosum and the wild diploid potato species S. kurtzianum, allotetraploid lines derived from this interspecific hybrid and S. kurtzianum autotetraploid lines (ktz-autotetraploid) using the S-SAP (sequence-specific amplified polymorphism) technique and normalized copy number determination by qPCR. Twenty-nine LTR-RT copies were activated in the hybrid and present in the allotetraploid lines. Major LTR-RT activity was detected in Copia-27, Copia-12, Copia-14 and, Gypsy-22. According to our results, LTR-RT copies were activated principally in the hybrid, there was no activation in allotetraploid lines and only one copy was activated in the autotetraploid. |
| format | Artículo |
| id | INTA10592 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | The Botanical Society of Japan |
| publisherStr | The Botanical Society of Japan |
| record_format | dspace |
| spelling | INTA105922024-04-22T10:24:20Z Hybridization and polyploidization effects on LTR‑retrotransposon activation in potato genome Gantuz, Magdalena Morales Sanfurgo, Hugo Andres Bertoldi, Maria Victoria Ibañez, Verónica Noé Duarte, Paola Fernanda Marfil, Carlos Federico Masuelli, Ricardo Williams Papa Hibridación Poliploidia Potatoes Hybridation Polyploidia Solanum tuberosum Long Terminal Repeat Retrotransposons Hybridization and polyploidization are major forces in plant evolution and potatoes are not an exception. It is proposed that the proliferation of Long Terminal Repeat-retrotransposons (LTR-RT) is related to genome reorganization caused by hybridization and/or polyploidization. The main purpose of the present work was to evaluate the effect of interspecific hybridization and polyploidization on the activation of LTR-RT. We evaluated the proliferation of putative active LTR-RT in a diploid hybrid between the cultivated potato Solanum tuberosum and the wild diploid potato species S. kurtzianum, allotetraploid lines derived from this interspecific hybrid and S. kurtzianum autotetraploid lines (ktz-autotetraploid) using the S-SAP (sequence-specific amplified polymorphism) technique and normalized copy number determination by qPCR. Twenty-nine LTR-RT copies were activated in the hybrid and present in the allotetraploid lines. Major LTR-RT activity was detected in Copia-27, Copia-12, Copia-14 and, Gypsy-22. According to our results, LTR-RT copies were activated principally in the hybrid, there was no activation in allotetraploid lines and only one copy was activated in the autotetraploid. EEA Mendoza Fil: Gantuz, Magdalena. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Morales Sanfurgo, Hugo Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Bertoldi, Maria Victoria. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Ibañez, Veronica Noe. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Duarte, Paola Fernanda. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Marfil, Carlos Federico. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Masuelli, Ricardo William. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Agrícola de Mendoza; Argentina 2021-10-26T14:40:19Z 2021-10-26T14:40:19Z 2021-10-26 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10592 https://link.springer.com/article/10.1007%2Fs10265-021-01354-9 0918-9440 1618-0860 https://doi.org/10.1007/s10265-021-01354-9 eng info:eu-repo/semantics/restrictedAccess application/pdf The Botanical Society of Japan Journal of Plant Research (Published: 21 October 2021) |
| spellingShingle | Papa Hibridación Poliploidia Potatoes Hybridation Polyploidia Solanum tuberosum Long Terminal Repeat Retrotransposons Gantuz, Magdalena Morales Sanfurgo, Hugo Andres Bertoldi, Maria Victoria Ibañez, Verónica Noé Duarte, Paola Fernanda Marfil, Carlos Federico Masuelli, Ricardo Williams Hybridization and polyploidization effects on LTR‑retrotransposon activation in potato genome |
| title | Hybridization and polyploidization effects on LTR‑retrotransposon activation in potato genome |
| title_full | Hybridization and polyploidization effects on LTR‑retrotransposon activation in potato genome |
| title_fullStr | Hybridization and polyploidization effects on LTR‑retrotransposon activation in potato genome |
| title_full_unstemmed | Hybridization and polyploidization effects on LTR‑retrotransposon activation in potato genome |
| title_short | Hybridization and polyploidization effects on LTR‑retrotransposon activation in potato genome |
| title_sort | hybridization and polyploidization effects on ltr retrotransposon activation in potato genome |
| topic | Papa Hibridación Poliploidia Potatoes Hybridation Polyploidia Solanum tuberosum Long Terminal Repeat Retrotransposons |
| url | http://hdl.handle.net/20.500.12123/10592 https://link.springer.com/article/10.1007%2Fs10265-021-01354-9 https://doi.org/10.1007/s10265-021-01354-9 |
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