Bot-graphy: An original technique for plant anatomy study based o metallography

Plant anatomists are recurrently looking for safer and faster methods to improve the observations of plants tissues, searching for images with better resolution. Nevertheless, some plant materials are difficult to handle due to their consistency, such as those with very hard tissue or with a combina...

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Main Authors: Montero, Ricardo, Gomez, Fernando, Setten, Lorena, Favret, Eduardo Alfredo, Torres, Dario
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Cambridge University Press 2021
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/10542
https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/botgraphy-an-original-technique-for-plant-anatomy-study-based-on-metallography/5A183F560E51146E4903FE03356442FE
https://doi.org/10.1017/S1431927621004323
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author Montero, Ricardo
Gomez, Fernando
Setten, Lorena
Favret, Eduardo Alfredo
Torres, Dario
author_browse Favret, Eduardo Alfredo
Gomez, Fernando
Montero, Ricardo
Setten, Lorena
Torres, Dario
author_facet Montero, Ricardo
Gomez, Fernando
Setten, Lorena
Favret, Eduardo Alfredo
Torres, Dario
author_sort Montero, Ricardo
collection INTA Digital
description Plant anatomists are recurrently looking for safer and faster methods to improve the observations of plants tissues, searching for images with better resolution. Nevertheless, some plant materials are difficult to handle due to their consistency, such as those with very hard tissue or with a combination of soft and hard tissues (e. g. parenchyma and sclerenchyma). In these cases, softening techniques have been developed [1]. But they do not completely solve this problem since their success depends on the thickness of the plant organ (Argentinian anatomists, pers. commun., 2020). To find a solution to this limitation, a standard procedure of metallography is proposed, as an alternative, for the study of plant organs. The standard technique consists of mounting the specimen in a castable resin (liquid polymer film), followed by grinding the sample with abrasive papers of different granulometry and observing the sample surface with optical reflection microscopy [2]. In this work, an example is shown, the basal internodes of 2.5 to 3 cm in diameter of sugarcane, harvested in Famaillá, Tucumán, Argentina.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
publishDate 2021
publishDateRange 2021
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publisher Cambridge University Press
publisherStr Cambridge University Press
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spelling INTA105422021-10-21T11:39:27Z Bot-graphy: An original technique for plant anatomy study based o metallography Montero, Ricardo Gomez, Fernando Setten, Lorena Favret, Eduardo Alfredo Torres, Dario Anatomía de la Planta Plant Anatomy Analytical Methods Técnicas Analíticas Microscopia de Reflexión Metalografia Saccharum offinalis Reflection Microscopy Metallography Plant anatomists are recurrently looking for safer and faster methods to improve the observations of plants tissues, searching for images with better resolution. Nevertheless, some plant materials are difficult to handle due to their consistency, such as those with very hard tissue or with a combination of soft and hard tissues (e. g. parenchyma and sclerenchyma). In these cases, softening techniques have been developed [1]. But they do not completely solve this problem since their success depends on the thickness of the plant organ (Argentinian anatomists, pers. commun., 2020). To find a solution to this limitation, a standard procedure of metallography is proposed, as an alternative, for the study of plant organs. The standard technique consists of mounting the specimen in a castable resin (liquid polymer film), followed by grinding the sample with abrasive papers of different granulometry and observing the sample surface with optical reflection microscopy [2]. In this work, an example is shown, the basal internodes of 2.5 to 3 cm in diameter of sugarcane, harvested in Famaillá, Tucumán, Argentina. Fil: Montero, Ricardo. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina Fil: Gomez, Fernando. Universidad Nacional de San Martin. Instituto de Tecnología Sabato; Argentina Fil: Setten, Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina Fil: Favret, Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina Fil: Torres, Dario. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina 2021-10-21T11:21:44Z 2021-10-21T11:21:44Z 2021-07-30 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10542 https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/botgraphy-an-original-technique-for-plant-anatomy-study-based-on-metallography/5A183F560E51146E4903FE03356442FE 1431-9276 1435-8115 https://doi.org/10.1017/S1431927621004323 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Cambridge University Press Microscopy and Microanalysis 27, Supplement S1 : 1146 -1147 (August 2021)
spellingShingle Anatomía de la Planta
Plant Anatomy
Analytical Methods
Técnicas Analíticas
Microscopia de Reflexión
Metalografia
Saccharum offinalis
Reflection Microscopy
Metallography
Montero, Ricardo
Gomez, Fernando
Setten, Lorena
Favret, Eduardo Alfredo
Torres, Dario
Bot-graphy: An original technique for plant anatomy study based o metallography
title Bot-graphy: An original technique for plant anatomy study based o metallography
title_full Bot-graphy: An original technique for plant anatomy study based o metallography
title_fullStr Bot-graphy: An original technique for plant anatomy study based o metallography
title_full_unstemmed Bot-graphy: An original technique for plant anatomy study based o metallography
title_short Bot-graphy: An original technique for plant anatomy study based o metallography
title_sort bot graphy an original technique for plant anatomy study based o metallography
topic Anatomía de la Planta
Plant Anatomy
Analytical Methods
Técnicas Analíticas
Microscopia de Reflexión
Metalografia
Saccharum offinalis
Reflection Microscopy
Metallography
url http://hdl.handle.net/20.500.12123/10542
https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/botgraphy-an-original-technique-for-plant-anatomy-study-based-on-metallography/5A183F560E51146E4903FE03356442FE
https://doi.org/10.1017/S1431927621004323
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