Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens
To study the in vivo distribution of the CNPV viral vector, we conducted two trials. In the first trial, six 11- day-old SPF chickens were intramuscularly vaccinated in one leg with CN048-VP2 (106 plaque-forming units (PFU) mixed with 0.5% v/v Indian ink). Three birds were sacrificed at 1 and 3 day...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1051 https://link.springer.com/article/10.1007%2Fs12250-015-3680-6 https://doi.org/10.1007/s12250-015-3680-6 |
| _version_ | 1855482809392562176 |
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| author | Zanetti, Flavia Adriana Cardona, Romina Federico, Carlos Rodolfo Chimeno Zoth, Silvina Andrea Calamante, Gabriela |
| author_browse | Calamante, Gabriela Cardona, Romina Chimeno Zoth, Silvina Andrea Federico, Carlos Rodolfo Zanetti, Flavia Adriana |
| author_facet | Zanetti, Flavia Adriana Cardona, Romina Federico, Carlos Rodolfo Chimeno Zoth, Silvina Andrea Calamante, Gabriela |
| author_sort | Zanetti, Flavia Adriana |
| collection | INTA Digital |
| description | To study the in vivo distribution of the CNPV viral vector, we conducted two trials. In the first trial, six 11- day-old SPF chickens were intramuscularly vaccinated in one leg with CN048-VP2 (106 plaque-forming units (PFU) mixed with 0.5% v/v Indian ink). Three birds were sacrificed at 1 and 3 days after vaccination, and muscle at the immunization site (identified by Indian ink), muscle from the non-injected leg, BF, and spleen were removed, weighed, and homogenized in phosphate buffer (50% w/v). Total DNA was extracted from organs using a QIAamp® DNA Mini Kit (QIAGEN, USA) and used as the template for PCR amplification of CNPV241 (primers P39F: 5′-AATAACACGACACAGCCGCAAG-3′, P39R: 5′-CAATTAATTAGATCGTGGTGGA-3′) or the glyceraldehyde-3-phosphate dehydrogenase (gapdh) gene (primers GAPDH Fw: 5′-AGAACATCATCCCAGCGTCC-3′, GAPDH Rv: 5′-CGGCAGGTCAGGTCAACA-3′). |
| format | Artículo |
| id | INTA1051 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA10512019-03-22T13:19:29Z Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens Zanetti, Flavia Adriana Cardona, Romina Federico, Carlos Rodolfo Chimeno Zoth, Silvina Andrea Calamante, Gabriela Enfermedades de los Animales Pollo Virus de los Animales Chickens Animal Diseases Animal Viruses Canarypox To study the in vivo distribution of the CNPV viral vector, we conducted two trials. In the first trial, six 11- day-old SPF chickens were intramuscularly vaccinated in one leg with CN048-VP2 (106 plaque-forming units (PFU) mixed with 0.5% v/v Indian ink). Three birds were sacrificed at 1 and 3 days after vaccination, and muscle at the immunization site (identified by Indian ink), muscle from the non-injected leg, BF, and spleen were removed, weighed, and homogenized in phosphate buffer (50% w/v). Total DNA was extracted from organs using a QIAamp® DNA Mini Kit (QIAGEN, USA) and used as the template for PCR amplification of CNPV241 (primers P39F: 5′-AATAACACGACACAGCCGCAAG-3′, P39R: 5′-CAATTAATTAGATCGTGGTGGA-3′) or the glyceraldehyde-3-phosphate dehydrogenase (gapdh) gene (primers GAPDH Fw: 5′-AGAACATCATCCCAGCGTCC-3′, GAPDH Rv: 5′-CGGCAGGTCAGGTCAACA-3′). Inst. de Biotecnología Fil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina Fil: Cardona, Romina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Federico, Carlos Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas: Argentina Fil: Chimeno Zoth, Silvina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Calamante, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2017-08-28T13:20:59Z 2017-08-28T13:20:59Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1051 https://link.springer.com/article/10.1007%2Fs12250-015-3680-6 1995-820X (Online) 1674-0769 (Print) https://doi.org/10.1007/s12250-015-3680-6 eng info:eu-repo/semantics/restrictedAccess application/pdf Virologica sinica 31(3) : 266–269. (June 2016) |
| spellingShingle | Enfermedades de los Animales Pollo Virus de los Animales Chickens Animal Diseases Animal Viruses Canarypox Zanetti, Flavia Adriana Cardona, Romina Federico, Carlos Rodolfo Chimeno Zoth, Silvina Andrea Calamante, Gabriela Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens |
| title | Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens |
| title_full | Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens |
| title_fullStr | Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens |
| title_full_unstemmed | Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens |
| title_short | Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens |
| title_sort | recombinant canarypox virus expressing the vp2 protein of infectious bursal disease virus induces protection in vaccinated spf chickens |
| topic | Enfermedades de los Animales Pollo Virus de los Animales Chickens Animal Diseases Animal Viruses Canarypox |
| url | http://hdl.handle.net/20.500.12123/1051 https://link.springer.com/article/10.1007%2Fs12250-015-3680-6 https://doi.org/10.1007/s12250-015-3680-6 |
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