Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation
The mechanisms by which the expression of animal cell death suppressors in economically important plants conferred enhanced stress tolerance are not fully understood. In the present work, the effect of expression of animal antiapoptotic gene Ced-9 in soybean hairy roots was evaluated under root hair...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1045 http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0101747&type=printable https://doi.org/10.1371/journal.pone.0101747 |
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| author | Robert, German Muñoz, Nacira Belen Melchiorre, Mariana Sanchez, Federico Lascano, Hernan Ramiro |
| author_browse | Lascano, Hernan Ramiro Melchiorre, Mariana Muñoz, Nacira Belen Robert, German Sanchez, Federico |
| author_facet | Robert, German Muñoz, Nacira Belen Melchiorre, Mariana Sanchez, Federico Lascano, Hernan Ramiro |
| author_sort | Robert, German |
| collection | INTA Digital |
| description | The mechanisms by which the expression of animal cell death suppressors in economically important plants conferred enhanced stress tolerance are not fully understood. In the present work, the effect of expression of animal antiapoptotic gene Ced-9 in soybean hairy roots was evaluated under root hairs and hairy roots death-inducing stress conditions given by i) Bradyrhizobium japonicum inoculation in presence of 50 mM NaCl, and ii) severe salt stress (150 mM NaCl), for 30 min and 3 h, respectively. We have determined that root hairs death induced by inoculation in presence of 50 mM NaCl showed
characteristics of ordered process, with increased ROS generation, MDA and ATP levels, whereas the cell death induced by 150 mM NaCl treatment showed non-ordered or necrotic-like characteristics. The expression of Ced-9 inhibited or at least
delayed root hairs death under these treatments. Hairy roots expressing Ced-9 had better homeostasis maintenance, preventing potassium release; increasing the ATP levels and controlling the oxidative damage avoiding the increase of
reactive oxygen species production. Even when our results demonstrate a positive effect of animal cell death suppressors in plant cell ionic and redox homeostasis under cell death-inducing conditions, its expression, contrary to expectations, drastically inhibited nodule formation even under control conditions. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1045 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA10452017-11-15T18:47:58Z Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation Robert, German Muñoz, Nacira Belen Melchiorre, Mariana Sanchez, Federico Lascano, Hernan Ramiro Glycine Max Soja Bradyrhizobium Japonicum Estrés Osmótico Genes Soybeans Osmotic Stress Anti-Apoptotic Gene Ced-9 Saline Stress Estrés Salino The mechanisms by which the expression of animal cell death suppressors in economically important plants conferred enhanced stress tolerance are not fully understood. In the present work, the effect of expression of animal antiapoptotic gene Ced-9 in soybean hairy roots was evaluated under root hairs and hairy roots death-inducing stress conditions given by i) Bradyrhizobium japonicum inoculation in presence of 50 mM NaCl, and ii) severe salt stress (150 mM NaCl), for 30 min and 3 h, respectively. We have determined that root hairs death induced by inoculation in presence of 50 mM NaCl showed characteristics of ordered process, with increased ROS generation, MDA and ATP levels, whereas the cell death induced by 150 mM NaCl treatment showed non-ordered or necrotic-like characteristics. The expression of Ced-9 inhibited or at least delayed root hairs death under these treatments. Hairy roots expressing Ced-9 had better homeostasis maintenance, preventing potassium release; increasing the ATP levels and controlling the oxidative damage avoiding the increase of reactive oxygen species production. Even when our results demonstrate a positive effect of animal cell death suppressors in plant cell ionic and redox homeostasis under cell death-inducing conditions, its expression, contrary to expectations, drastically inhibited nodule formation even under control conditions. Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina 2017-08-25T16:48:20Z 2017-08-25T16:48:20Z 2014-07-27 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1045 http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0101747&type=printable :Robert G, Muñoz N, Melchiorre M, Sánchez F, Lascano R (2014) Expression of Animal Anti-Apoptotic Gene Ced-9 Enhances Tolerance during Glycine max L.–Bradyrhizobium japonicum Interaction under Saline Stress but Reduces Nodule Formation. PLoS ONE 9(7): e101747. doi:10.1371/journal.pone.0101747 1932-6203 https://doi.org/10.1371/journal.pone.0101747 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf |
| spellingShingle | Glycine Max Soja Bradyrhizobium Japonicum Estrés Osmótico Genes Soybeans Osmotic Stress Anti-Apoptotic Gene Ced-9 Saline Stress Estrés Salino Robert, German Muñoz, Nacira Belen Melchiorre, Mariana Sanchez, Federico Lascano, Hernan Ramiro Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation |
| title | Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation |
| title_full | Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation |
| title_fullStr | Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation |
| title_full_unstemmed | Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation |
| title_short | Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation |
| title_sort | expression of animal anti apoptotic gene ced 9 enhances tolerance during glycine max l bradyrhizobium japonicum interaction under saline stress but reduces nodule formation |
| topic | Glycine Max Soja Bradyrhizobium Japonicum Estrés Osmótico Genes Soybeans Osmotic Stress Anti-Apoptotic Gene Ced-9 Saline Stress Estrés Salino |
| url | http://hdl.handle.net/20.500.12123/1045 http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0101747&type=printable https://doi.org/10.1371/journal.pone.0101747 |
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