Role of calcium in the defense response induced by brassinosteroids in strawberry plants
Brassinosteroids (BRs) are steroidal hormones essential for the normal growth and development of plants. The aim of this work was to study the role of calcium, in the defense response induced by two BRs e.g. BB16 and EP24, and to investigate the expression levels of defense-related genes in strawber...
| Main Authors: | , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Elsevier
2021
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/10410 https://www.sciencedirect.com/science/article/pii/S0304423819308969 https://doi.org/10.1016/j.scienta.2019.109010 |
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| author | Furio, Ramiro Nicolás Martinez Zamora, Martin Gustavo Salazar, Sergio Miguel Coll, Yamilet Perato, Silvia Marisa Martos, Gustavo Gabriel Díaz Ricci, Juan Carlos |
| author_browse | Coll, Yamilet Díaz Ricci, Juan Carlos Furio, Ramiro Nicolás Martinez Zamora, Martin Gustavo Martos, Gustavo Gabriel Perato, Silvia Marisa Salazar, Sergio Miguel |
| author_facet | Furio, Ramiro Nicolás Martinez Zamora, Martin Gustavo Salazar, Sergio Miguel Coll, Yamilet Perato, Silvia Marisa Martos, Gustavo Gabriel Díaz Ricci, Juan Carlos |
| author_sort | Furio, Ramiro Nicolás |
| collection | INTA Digital |
| description | Brassinosteroids (BRs) are steroidal hormones essential for the normal growth and development of plants. The aim of this work was to study the role of calcium, in the defense response induced by two BRs e.g. BB16 and EP24, and to investigate the expression levels of defense-related genes in strawberry plants treated with these BRs. An increase in the calcium influx in response to treatment with BB16 or EP24 was observed. By comparing Fragaria vesca and Fragaria ananassa genome sequences 28 nucleotide sequences coding for calcium-sensing proteins called CMLs were predicted. Bioinformatic analyses of the sequences revealed that all of them contained the characteristic EF-hand motif of Ca2+ binding, and regulatory cis-elements related to stress. qPCR studies showed that both BB16 and EP24 cause the upregulation of selected CMLs genes e.g. FaCML1a, FaCML36, FaCML42 and FaCML45, and defense marker genes, such as PR1, ERF1 and GLS5. Results also showed that whereas BB16 induced the upregulation of MYB30, EP24 induced the upregulation of PRX27. These results provide valuable information of the response mechanisms induced by BRs and demonstrate the role of calcium in the signaling pathways induced by BRs in strawberry plants. |
| format | Artículo |
| id | INTA10410 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA104102021-10-04T12:44:37Z Role of calcium in the defense response induced by brassinosteroids in strawberry plants Furio, Ramiro Nicolás Martinez Zamora, Martin Gustavo Salazar, Sergio Miguel Coll, Yamilet Perato, Silvia Marisa Martos, Gustavo Gabriel Díaz Ricci, Juan Carlos Fresa Fragaria ananassa Brasinoesteroides Calcio Calmodulina Strawberries Brassinosteroids Calcium Calmodulin Immune Response Respuesta Inmunológica Frutilla Brassinosteroids (BRs) are steroidal hormones essential for the normal growth and development of plants. The aim of this work was to study the role of calcium, in the defense response induced by two BRs e.g. BB16 and EP24, and to investigate the expression levels of defense-related genes in strawberry plants treated with these BRs. An increase in the calcium influx in response to treatment with BB16 or EP24 was observed. By comparing Fragaria vesca and Fragaria ananassa genome sequences 28 nucleotide sequences coding for calcium-sensing proteins called CMLs were predicted. Bioinformatic analyses of the sequences revealed that all of them contained the characteristic EF-hand motif of Ca2+ binding, and regulatory cis-elements related to stress. qPCR studies showed that both BB16 and EP24 cause the upregulation of selected CMLs genes e.g. FaCML1a, FaCML36, FaCML42 and FaCML45, and defense marker genes, such as PR1, ERF1 and GLS5. Results also showed that whereas BB16 induced the upregulation of MYB30, EP24 induced the upregulation of PRX27. These results provide valuable information of the response mechanisms induced by BRs and demonstrate the role of calcium in the signaling pathways induced by BRs in strawberry plants. EEA Famaillá Fil: Furio, R.N. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Furio, R.N. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Martinez Zamora, Martin Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Martinez Zamora, Martin Gustavo. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Salazar, Sergio Miguel. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina Fil: Coll, Yamilet. Universidad La Habana. Centro de Estudio de Productos Naturales; Cuba Fil: Perato, Silvia Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Perato, Silvia Marisa. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Martos, Gustavo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Martos, Gustavo Gabriel. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Diaz Ricci, Juan Carlos. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. 2021-10-04T12:41:04Z 2021-10-04T12:41:04Z 2020-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10410 https://www.sciencedirect.com/science/article/pii/S0304423819308969 0304-4238 https://doi.org/10.1016/j.scienta.2019.109010 eng info:eu-repograntAgreement/INTA/PNHFA-1106073/AR./Aumento de la competitividad con sustentabilidad y equidad social de sistemas productivos de hortalizas frescas diferenciadas. info:eu-repograntAgreement/INTA/TUSGO-1231101/AR./Desarrollo sustentable de los territorios a traves de procesos de innovación tecnológica, socio organizativa e institucional del área norte de la provincia de Tucumán. info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Scientia Horticulturae 261 : 109010 (February 2020) |
| spellingShingle | Fresa Fragaria ananassa Brasinoesteroides Calcio Calmodulina Strawberries Brassinosteroids Calcium Calmodulin Immune Response Respuesta Inmunológica Frutilla Furio, Ramiro Nicolás Martinez Zamora, Martin Gustavo Salazar, Sergio Miguel Coll, Yamilet Perato, Silvia Marisa Martos, Gustavo Gabriel Díaz Ricci, Juan Carlos Role of calcium in the defense response induced by brassinosteroids in strawberry plants |
| title | Role of calcium in the defense response induced by brassinosteroids in strawberry plants |
| title_full | Role of calcium in the defense response induced by brassinosteroids in strawberry plants |
| title_fullStr | Role of calcium in the defense response induced by brassinosteroids in strawberry plants |
| title_full_unstemmed | Role of calcium in the defense response induced by brassinosteroids in strawberry plants |
| title_short | Role of calcium in the defense response induced by brassinosteroids in strawberry plants |
| title_sort | role of calcium in the defense response induced by brassinosteroids in strawberry plants |
| topic | Fresa Fragaria ananassa Brasinoesteroides Calcio Calmodulina Strawberries Brassinosteroids Calcium Calmodulin Immune Response Respuesta Inmunológica Frutilla |
| url | http://hdl.handle.net/20.500.12123/10410 https://www.sciencedirect.com/science/article/pii/S0304423819308969 https://doi.org/10.1016/j.scienta.2019.109010 |
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