The Neospora caninum-Spain 7 isolate induces placental damage, fetal death and abortion in cattle when inoculated in early gestation
The Nc-Spain 7 isolate of Neospora caninum, which was newly obtained from an asymptomatic congenitally infected calf, demonstrated a similar virulence as Nc-1 strain in mouse models. The aim of this study was to characterize the pathogenesis of Nc-Spain 7 isolate in cattle after experimental infecti...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/10404 https://www.sciencedirect.com/science/article/abs/pii/S0304401712002464 https://doi.org/10.1016/j.vetpar.2012.04.034 |
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| author | Caspe, Sergio Gaston Moore, Prando Dadin Leunda, Maria Rosa Cano, Dora Beatriz Lischinsky, Lilian Haydee Regidor-Cerrillo, Javier Alvarez-García, Gema Echaide, Ignacio Eduardo Bacigalupe, Diana Ortega-Mora, Luis Odeon, Anselmo Carlos Campero, Carlos Manuel |
| author_browse | Alvarez-García, Gema Bacigalupe, Diana Campero, Carlos Manuel Cano, Dora Beatriz Caspe, Sergio Gaston Echaide, Ignacio Eduardo Leunda, Maria Rosa Lischinsky, Lilian Haydee Moore, Prando Dadin Odeon, Anselmo Carlos Ortega-Mora, Luis Regidor-Cerrillo, Javier |
| author_facet | Caspe, Sergio Gaston Moore, Prando Dadin Leunda, Maria Rosa Cano, Dora Beatriz Lischinsky, Lilian Haydee Regidor-Cerrillo, Javier Alvarez-García, Gema Echaide, Ignacio Eduardo Bacigalupe, Diana Ortega-Mora, Luis Odeon, Anselmo Carlos Campero, Carlos Manuel |
| author_sort | Caspe, Sergio Gaston |
| collection | INTA Digital |
| description | The Nc-Spain 7 isolate of Neospora caninum, which was newly obtained from an asymptomatic congenitally infected calf, demonstrated a similar virulence as Nc-1 strain in mouse models. The aim of this study was to characterize the pathogenesis of Nc-Spain 7 isolate in cattle after experimental infection at 65 days of gestation. For this purpose, thirteen pregnant heifers were divided into three groups as follows: group A: 7 heifers inoculated with 1 × 108 tachyzoites of Nc-Spain 7 isolate; group B: 4 heifers inoculated with 1 × 108 tachyzoites of Nc 1 strain; and group C: 2 heifers received PBS. Serum samples were collected weekly and heparinized blood samples were collected three times (0, 28 and 42 days after inoculation) by jugular venipuncture. Placenta and fetal tissue samples were collected at time of necropsy. Specific antibody response in the dams was tested by IFAT, indirect ELISA, and rNcGRA7 and rNcSAG4 based-ELISA. Specific antibody response in fetal fluids was tested by IFAT. IFN-γ production was measured after in vitro culture of PBMC and the supernatant was assessed using a commercial kit (BOVIGAM). A significant increase in N. caninum antibody responses was detected in groups A and B by IFAT and by i-ELISA from day 14 after inoculation onwards. Besides, antibody response against rNCGra7 protein was also detected in all inoculated heifers by rNcGra7-based ELISA. Four fetuses from group A and one from group B were aborted between 3 and 5 weeks after infection. In the recovered fetuses, only 3 out of 4 fetal fluids from fetuses of group A and 1 out of 3 of group B were seropositive by IFAT, but all of them were positive by PCR. Transplacental transmission could be determined in all fetuses from groups A and B by PCR and/or IHC. Heifers of group C and their fetuses remained negative by all techniques. The results of this study demonstrate that the NC-Spain 7 isolate could be transmitted transplacentally, and produced fetal death and abortion in cattle. |
| format | info:ar-repo/semantics/artículo |
| id | INTA10404 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA104042021-10-01T12:35:15Z The Neospora caninum-Spain 7 isolate induces placental damage, fetal death and abortion in cattle when inoculated in early gestation Caspe, Sergio Gaston Moore, Prando Dadin Leunda, Maria Rosa Cano, Dora Beatriz Lischinsky, Lilian Haydee Regidor-Cerrillo, Javier Alvarez-García, Gema Echaide, Ignacio Eduardo Bacigalupe, Diana Ortega-Mora, Luis Odeon, Anselmo Carlos Campero, Carlos Manuel Ganado Bovino Neospora caninum Aborto Gestación Enfermedades de los Animales Placenta Cattle Abortion Pregnancy Animal Diseases The Nc-Spain 7 isolate of Neospora caninum, which was newly obtained from an asymptomatic congenitally infected calf, demonstrated a similar virulence as Nc-1 strain in mouse models. The aim of this study was to characterize the pathogenesis of Nc-Spain 7 isolate in cattle after experimental infection at 65 days of gestation. For this purpose, thirteen pregnant heifers were divided into three groups as follows: group A: 7 heifers inoculated with 1 × 108 tachyzoites of Nc-Spain 7 isolate; group B: 4 heifers inoculated with 1 × 108 tachyzoites of Nc 1 strain; and group C: 2 heifers received PBS. Serum samples were collected weekly and heparinized blood samples were collected three times (0, 28 and 42 days after inoculation) by jugular venipuncture. Placenta and fetal tissue samples were collected at time of necropsy. Specific antibody response in the dams was tested by IFAT, indirect ELISA, and rNcGRA7 and rNcSAG4 based-ELISA. Specific antibody response in fetal fluids was tested by IFAT. IFN-γ production was measured after in vitro culture of PBMC and the supernatant was assessed using a commercial kit (BOVIGAM). A significant increase in N. caninum antibody responses was detected in groups A and B by IFAT and by i-ELISA from day 14 after inoculation onwards. Besides, antibody response against rNCGra7 protein was also detected in all inoculated heifers by rNcGra7-based ELISA. Four fetuses from group A and one from group B were aborted between 3 and 5 weeks after infection. In the recovered fetuses, only 3 out of 4 fetal fluids from fetuses of group A and 1 out of 3 of group B were seropositive by IFAT, but all of them were positive by PCR. Transplacental transmission could be determined in all fetuses from groups A and B by PCR and/or IHC. Heifers of group C and their fetuses remained negative by all techniques. The results of this study demonstrate that the NC-Spain 7 isolate could be transmitted transplacentally, and produced fetal death and abortion in cattle. EEA Mercedes Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Moore, Prando Dadin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Cano, Dora Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Lischinsky, Lilian Haydee. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Regidor-Cerrillo, Javier. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias. Departamento de Salud Animal. SALUVET; España Fil: Alvarez-García, Gema. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias. Departamento de Salud Animal. SALUVET; España Fil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Bacigalupe, Diana. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina Fil: Ortega-Mora, Luis M. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias. Departamento de Salud Animal. SALUVET; España Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina 2021-10-01T12:29:34Z 2021-10-01T12:29:34Z 2012-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10404 https://www.sciencedirect.com/science/article/abs/pii/S0304401712002464 0304-4017 https://doi.org/10.1016/j.vetpar.2012.04.034 eng info:eu-repograntAgreement/INTA/AESA-203971/AR./Caracterización y control de las pérdidas gestacionales y perinatales por causas infecciosas en bovinos info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Parasitology 189 (2-4) : 171-181. (October 2012) |
| spellingShingle | Ganado Bovino Neospora caninum Aborto Gestación Enfermedades de los Animales Placenta Cattle Abortion Pregnancy Animal Diseases Caspe, Sergio Gaston Moore, Prando Dadin Leunda, Maria Rosa Cano, Dora Beatriz Lischinsky, Lilian Haydee Regidor-Cerrillo, Javier Alvarez-García, Gema Echaide, Ignacio Eduardo Bacigalupe, Diana Ortega-Mora, Luis Odeon, Anselmo Carlos Campero, Carlos Manuel The Neospora caninum-Spain 7 isolate induces placental damage, fetal death and abortion in cattle when inoculated in early gestation |
| title | The Neospora caninum-Spain 7 isolate induces placental damage, fetal death and abortion in cattle when inoculated in early gestation |
| title_full | The Neospora caninum-Spain 7 isolate induces placental damage, fetal death and abortion in cattle when inoculated in early gestation |
| title_fullStr | The Neospora caninum-Spain 7 isolate induces placental damage, fetal death and abortion in cattle when inoculated in early gestation |
| title_full_unstemmed | The Neospora caninum-Spain 7 isolate induces placental damage, fetal death and abortion in cattle when inoculated in early gestation |
| title_short | The Neospora caninum-Spain 7 isolate induces placental damage, fetal death and abortion in cattle when inoculated in early gestation |
| title_sort | neospora caninum spain 7 isolate induces placental damage fetal death and abortion in cattle when inoculated in early gestation |
| topic | Ganado Bovino Neospora caninum Aborto Gestación Enfermedades de los Animales Placenta Cattle Abortion Pregnancy Animal Diseases |
| url | http://hdl.handle.net/20.500.12123/10404 https://www.sciencedirect.com/science/article/abs/pii/S0304401712002464 https://doi.org/10.1016/j.vetpar.2012.04.034 |
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