A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation
Water buffalo (Bubalus bubalis) and domestic cattle (Bos taurus) are closely related species. However, embryo transfer interspecies has been attempted without any success. The failure in hybrid embryo-implantation is associated with the glycocode in the maternal-fetal interface. Glycosylation patter...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Tech Science Press
2021
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/10395 https://www.techscience.com/biocell/v45n2/41546 http://dx.doi.org/10.32604/biocell.2021.010701 |
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| author | Caspe, Sergio Gaston Konrad, Jose Luis Moore, Prando Dadin Sala, Juan Manuel Lischinsky, Lilian Haydee Campero, Carlos Manuel Barbeito, Claudio Gustavo |
| author_browse | Barbeito, Claudio Gustavo Campero, Carlos Manuel Caspe, Sergio Gaston Konrad, Jose Luis Lischinsky, Lilian Haydee Moore, Prando Dadin Sala, Juan Manuel |
| author_facet | Caspe, Sergio Gaston Konrad, Jose Luis Moore, Prando Dadin Sala, Juan Manuel Lischinsky, Lilian Haydee Campero, Carlos Manuel Barbeito, Claudio Gustavo |
| author_sort | Caspe, Sergio Gaston |
| collection | INTA Digital |
| description | Water buffalo (Bubalus bubalis) and domestic cattle (Bos taurus) are closely related species. However, embryo transfer interspecies has been attempted without any success. The failure in hybrid embryo-implantation is associated with the glycocode in the maternal-fetal interface. Glycosylation patterns have been studied in different species of ruminants; however, in B. bubalis, only the binucleated cells (BNC) have been analyzed. This glycocode is essential for a successful embryo-implantation and can be defined by Lectin-Histochemistry (LHC). The aim of this study is to compare the glycosylation pattern of placenta and uterus in water buffaloes and cattle by LHC. Tissue samples of placenta and uterus from pregnant Mediterranean female water buffaloes (Buf1) and Angus cows (Bov1) were analyzed. All animals were euthanized at 98 days of gestation. LHC was carried out using twelve lectins (Con A, LCA, PSA, sWGA, PHA-e, SBA, UEA-1, WGA, RCA-1, PNA, DBA, BSA-1). The intensity of lectin binding was semiquantitatively scored using a scale of 0 (negative) to 3 (strongly positive). Difference between species was found in trophoblast layer by PSA, SWGA, PNA and BSA-1, in BNC, and in the mononuclear cells by LCA, PSA, PHA-e, DBA, BSA-1, PNA. In utero, differences in the apical cellular membrane and the secretion of glands were identified by DBA and RCA-1, and in the cytoplasm of those glandular epithelial cells by PHA-e, BSA-1, WGA, and SBA. In both species, BNC presented a strong positive reaction with DBA and SBA, a moderate response by LCA, PHA-e, BSA-1 and PNA lectin, and a low reaction by PSA, UEA-1, SWGA, WGA, Con A and RCA-1. The results found in this study suggest that although both species are closely related, glycosylation patterns in the placenta and uterus are different, thus providing a possible reason for embryo transfer not being possible between these species. |
| format | info:ar-repo/semantics/artículo |
| id | INTA10395 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Tech Science Press |
| publisherStr | Tech Science Press |
| record_format | dspace |
| spelling | INTA103952021-09-30T14:17:23Z A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation Caspe, Sergio Gaston Konrad, Jose Luis Moore, Prando Dadin Sala, Juan Manuel Lischinsky, Lilian Haydee Campero, Carlos Manuel Barbeito, Claudio Gustavo Búfalo de Agua Ganado Bovino Lectinas Gestación Water Buffaloes Cattle Lectins Pregnancy Placenta Water buffalo (Bubalus bubalis) and domestic cattle (Bos taurus) are closely related species. However, embryo transfer interspecies has been attempted without any success. The failure in hybrid embryo-implantation is associated with the glycocode in the maternal-fetal interface. Glycosylation patterns have been studied in different species of ruminants; however, in B. bubalis, only the binucleated cells (BNC) have been analyzed. This glycocode is essential for a successful embryo-implantation and can be defined by Lectin-Histochemistry (LHC). The aim of this study is to compare the glycosylation pattern of placenta and uterus in water buffaloes and cattle by LHC. Tissue samples of placenta and uterus from pregnant Mediterranean female water buffaloes (Buf1) and Angus cows (Bov1) were analyzed. All animals were euthanized at 98 days of gestation. LHC was carried out using twelve lectins (Con A, LCA, PSA, sWGA, PHA-e, SBA, UEA-1, WGA, RCA-1, PNA, DBA, BSA-1). The intensity of lectin binding was semiquantitatively scored using a scale of 0 (negative) to 3 (strongly positive). Difference between species was found in trophoblast layer by PSA, SWGA, PNA and BSA-1, in BNC, and in the mononuclear cells by LCA, PSA, PHA-e, DBA, BSA-1, PNA. In utero, differences in the apical cellular membrane and the secretion of glands were identified by DBA and RCA-1, and in the cytoplasm of those glandular epithelial cells by PHA-e, BSA-1, WGA, and SBA. In both species, BNC presented a strong positive reaction with DBA and SBA, a moderate response by LCA, PHA-e, BSA-1 and PNA lectin, and a low reaction by PSA, UEA-1, SWGA, WGA, Con A and RCA-1. The results found in this study suggest that although both species are closely related, glycosylation patterns in the placenta and uterus are different, thus providing a possible reason for embryo transfer not being possible between these species. EEA Mercedes Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Konrad, Jose Luis. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina Fil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Sala, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Lischinsky, Lilian Haydee. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Barbeito, Claudio G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. 2021-09-30T14:14:06Z 2021-09-30T14:14:06Z 2021 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10395 https://www.techscience.com/biocell/v45n2/41546 0327-9545 1667-5746 http://dx.doi.org/10.32604/biocell.2021.010701 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Tech Science Press Biocell (Mendoza) 45 (2) : 331-337. (2021) |
| spellingShingle | Búfalo de Agua Ganado Bovino Lectinas Gestación Water Buffaloes Cattle Lectins Pregnancy Placenta Caspe, Sergio Gaston Konrad, Jose Luis Moore, Prando Dadin Sala, Juan Manuel Lischinsky, Lilian Haydee Campero, Carlos Manuel Barbeito, Claudio Gustavo A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation |
| title | A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation |
| title_full | A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation |
| title_fullStr | A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation |
| title_full_unstemmed | A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation |
| title_short | A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation |
| title_sort | comparative pattern of lectin binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation |
| topic | Búfalo de Agua Ganado Bovino Lectinas Gestación Water Buffaloes Cattle Lectins Pregnancy Placenta |
| url | http://hdl.handle.net/20.500.12123/10395 https://www.techscience.com/biocell/v45n2/41546 http://dx.doi.org/10.32604/biocell.2021.010701 |
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