DNA Barcoding of Phymaturus Lizards Reveals Conflicts in Species Delimitation within the patagonicus Clade
Under the DNA Barcode initiative, we used the mitochondrial locus cytochrome c oxidase I to test if this molecular marker would reliably distinguish among lizard species of the patagonicus clade of Phymaturus. Using 18 described species and two populations of unidentified species, we calculated intr...
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Society for the Study of Amphibians and Reptiles
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/10389 https://bioone.org/journals/journal-of-herpetology/volume-50/issue-4/15-104/DNA-Barcoding-of-Phymaturus-Lizards-Reveals-Conflicts-in-Species-Delimitation/10.1670/15-104.short https://doi.org/10.1670/15-104 |
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| author | Corbalán, Valeria Debandi, Guillermo Oscar Scolaro, José Alejandro Ojeda, Agustina A. |
| author_browse | Corbalán, Valeria Debandi, Guillermo Oscar Ojeda, Agustina A. Scolaro, José Alejandro |
| author_facet | Corbalán, Valeria Debandi, Guillermo Oscar Scolaro, José Alejandro Ojeda, Agustina A. |
| author_sort | Corbalán, Valeria |
| collection | INTA Digital |
| description | Under the DNA Barcode initiative, we used the mitochondrial locus cytochrome c oxidase I to test if this molecular marker would reliably distinguish among lizard species of the patagonicus clade of Phymaturus. Using 18 described species and two populations of unidentified species, we calculated intra- and interpopulation genetic distances for all operational taxonomic units and performed phylogenetic reconstructions using maximum parsimony and maximum likelihood. We identified different species that share the same barcode index number (BIN). We recorded only 12 of the 18 previously described species and one candidate species from the new population. By comparing our results with published morphological and molecular phylogenies, as well as with previous debates, we propose possible explanations for this. In some cases (such as the group with the same BIN formed by Phymaturus spurcus, Phymaturus spectabilis, Phymaturus excelsus, and Phymaturus agilis), where other authors debated the identity of the species, we suggest that the low genetic distances could be attributable to the presence of one species with high polymorphism. On the other hand, in geographically isolated species such as the group formed by Phymaturus payuniae and Phymaturus nevadoi, the group formed by Phymaturus somuncurensis and Phymaturus ceii, and the group formed by Phymaturus indistinctus and Phymaturus videlai, the topology of the phylogenetic trees indicates that the low genetic distances (also found by other authors analyzing cytochrome b) could be attributable to shared ancestral polymorphism resulting from incomplete lineage sorting. |
| format | Artículo |
| id | INTA10389 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Society for the Study of Amphibians and Reptiles |
| publisherStr | Society for the Study of Amphibians and Reptiles |
| record_format | dspace |
| spelling | INTA103892021-09-29T14:33:27Z DNA Barcoding of Phymaturus Lizards Reveals Conflicts in Species Delimitation within the patagonicus Clade Corbalán, Valeria Debandi, Guillermo Oscar Scolaro, José Alejandro Ojeda, Agustina A. Lagarto ADN Identificación Lizards DNA Identification Phymaturus Under the DNA Barcode initiative, we used the mitochondrial locus cytochrome c oxidase I to test if this molecular marker would reliably distinguish among lizard species of the patagonicus clade of Phymaturus. Using 18 described species and two populations of unidentified species, we calculated intra- and interpopulation genetic distances for all operational taxonomic units and performed phylogenetic reconstructions using maximum parsimony and maximum likelihood. We identified different species that share the same barcode index number (BIN). We recorded only 12 of the 18 previously described species and one candidate species from the new population. By comparing our results with published morphological and molecular phylogenies, as well as with previous debates, we propose possible explanations for this. In some cases (such as the group with the same BIN formed by Phymaturus spurcus, Phymaturus spectabilis, Phymaturus excelsus, and Phymaturus agilis), where other authors debated the identity of the species, we suggest that the low genetic distances could be attributable to the presence of one species with high polymorphism. On the other hand, in geographically isolated species such as the group formed by Phymaturus payuniae and Phymaturus nevadoi, the group formed by Phymaturus somuncurensis and Phymaturus ceii, and the group formed by Phymaturus indistinctus and Phymaturus videlai, the topology of the phylogenetic trees indicates that the low genetic distances (also found by other authors analyzing cytochrome b) could be attributable to shared ancestral polymorphism resulting from incomplete lineage sorting. EEA Junín Fil: Corbalán, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina Fil: Corbalán, Valeria. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina. Fil: Debandi, Guillermo Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina. Fil: Scolaro, José Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina Fil: Scolaro, José Alejandro. Universidad Nacional de la Patagonia San Juan Bosco. Facultad de Ciencias Naturales; Argentina Fil: Ojeda, Agustina A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina Fil: Ojeda, Agustina A. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina. 2021-09-29T14:30:36Z 2021-09-29T14:30:36Z 2016-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10389 https://bioone.org/journals/journal-of-herpetology/volume-50/issue-4/15-104/DNA-Barcoding-of-Phymaturus-Lizards-Reveals-Conflicts-in-Species-Delimitation/10.1670/15-104.short 0022-1511 1937-2418 https://doi.org/10.1670/15-104 eng info:eu-repo/semantics/restrictedAccess application/pdf Society for the Study of Amphibians and Reptiles Journal of Herpetology 50 (4) : 654-666. (2016) |
| spellingShingle | Lagarto ADN Identificación Lizards DNA Identification Phymaturus Corbalán, Valeria Debandi, Guillermo Oscar Scolaro, José Alejandro Ojeda, Agustina A. DNA Barcoding of Phymaturus Lizards Reveals Conflicts in Species Delimitation within the patagonicus Clade |
| title | DNA Barcoding of Phymaturus Lizards Reveals Conflicts in Species Delimitation within the patagonicus Clade |
| title_full | DNA Barcoding of Phymaturus Lizards Reveals Conflicts in Species Delimitation within the patagonicus Clade |
| title_fullStr | DNA Barcoding of Phymaturus Lizards Reveals Conflicts in Species Delimitation within the patagonicus Clade |
| title_full_unstemmed | DNA Barcoding of Phymaturus Lizards Reveals Conflicts in Species Delimitation within the patagonicus Clade |
| title_short | DNA Barcoding of Phymaturus Lizards Reveals Conflicts in Species Delimitation within the patagonicus Clade |
| title_sort | dna barcoding of phymaturus lizards reveals conflicts in species delimitation within the patagonicus clade |
| topic | Lagarto ADN Identificación Lizards DNA Identification Phymaturus |
| url | http://hdl.handle.net/20.500.12123/10389 https://bioone.org/journals/journal-of-herpetology/volume-50/issue-4/15-104/DNA-Barcoding-of-Phymaturus-Lizards-Reveals-Conflicts-in-Species-Delimitation/10.1670/15-104.short https://doi.org/10.1670/15-104 |
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