Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stages
Lead (Pb) contamination of agricultural soils, and subsequently of crops, has been widely reported. Soybean (Glycine max (L.) Merr.) has been indicated as a plant that accumulates Pb, even in soils that do not exceed the maximum permissible levels. Considering the toxicity of this heavy metal, the a...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/10333 https://link.springer.com/article/10.1007/s11356-021-12389-9 https://doi.org/10.1007/s11356-021-12389-9 |
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| author | Blanco, Andrés Pignata, María L. Lascano, Hernan Ramiro Rodriguez, Judith Hebelen |
| author_browse | Blanco, Andrés Lascano, Hernan Ramiro Pignata, María L. Rodriguez, Judith Hebelen |
| author_facet | Blanco, Andrés Pignata, María L. Lascano, Hernan Ramiro Rodriguez, Judith Hebelen |
| author_sort | Blanco, Andrés |
| collection | INTA Digital |
| description | Lead (Pb) contamination of agricultural soils, and subsequently of crops, has been widely reported. Soybean (Glycine max (L.) Merr.) has been indicated as a plant that accumulates Pb, even in soils that do not exceed the maximum permissible levels. Considering the toxicity of this heavy metal, the aim of the present study was to assess different concentrations of Pb, from low to extremely high (0.25 mM, 1 mM, and 2.5 mM), in soybean seedlings and their tolerance by analyzing morpho-physiological parameters in hydroponic experiments. Soybean seedlings were exposed to control and Pb treatments during 8 days, coinciding with the early growth stages, and the following variables were analyzed: biomass, Pb content in roots, stems and leaves, photosynthetic efficiency, leaf area, biochemical response (antioxidant power, chlorophylls, malondialdehyde), and relative water content of leaves. Results showed that roots accumulated much more Pb than the other organs, with Pb accumulation in roots being saturated even at the lowest Pb concentration, which was reflected in root biomass. Moreover, absorption of culture solutions was lower in Pb treatments, which was also reflected in the lower leaf relative water content. Lead toxicity symptoms in leaves (chlorosis and dark spots, and a decrease of biomass and leaf area, chlorophyll content, and photosynthetic efficiency), and an increase of the oxidative defense system were associated only with the highest Pb concentration (2.5 mM). Our findings support the evidence of soybean as a species tolerant to Pb, showing the effects of toxicity at very high concentrations. |
| format | Artículo |
| id | INTA10333 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA103332024-02-29T11:19:17Z Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stages Blanco, Andrés Pignata, María L. Lascano, Hernan Ramiro Rodriguez, Judith Hebelen Soybeans Toxicity Abiotic Stress Bioaccumulation Hydroponics Toxicidad Estrés Abiótico Glycine Max Soja Bioacumulación Cultivo Hidropónico Pb Morpho-Physiological Parameters Hydroponic System Lead (Pb) contamination of agricultural soils, and subsequently of crops, has been widely reported. Soybean (Glycine max (L.) Merr.) has been indicated as a plant that accumulates Pb, even in soils that do not exceed the maximum permissible levels. Considering the toxicity of this heavy metal, the aim of the present study was to assess different concentrations of Pb, from low to extremely high (0.25 mM, 1 mM, and 2.5 mM), in soybean seedlings and their tolerance by analyzing morpho-physiological parameters in hydroponic experiments. Soybean seedlings were exposed to control and Pb treatments during 8 days, coinciding with the early growth stages, and the following variables were analyzed: biomass, Pb content in roots, stems and leaves, photosynthetic efficiency, leaf area, biochemical response (antioxidant power, chlorophylls, malondialdehyde), and relative water content of leaves. Results showed that roots accumulated much more Pb than the other organs, with Pb accumulation in roots being saturated even at the lowest Pb concentration, which was reflected in root biomass. Moreover, absorption of culture solutions was lower in Pb treatments, which was also reflected in the lower leaf relative water content. Lead toxicity symptoms in leaves (chlorosis and dark spots, and a decrease of biomass and leaf area, chlorophyll content, and photosynthetic efficiency), and an increase of the oxidative defense system were associated only with the highest Pb concentration (2.5 mM). Our findings support the evidence of soybean as a species tolerant to Pb, showing the effects of toxicity at very high concentrations. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Blanco, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal (IMBIV). Área Contaminación y Bioindicadores; Argentina Fil: Blanco, Andrés. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina Fil: Pignata, María L. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal (IMBIV). Área Contaminación y Bioindicadores; Argentina Fil: Pignata, María L. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina Fil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales. Argentina Fil: Rodriguez, Judith H. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal (IMBIV). Área Contaminación y Bioindicadores. Argentina Fil: Rodriguez, Judith H. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina 2021-09-22T18:56:21Z 2021-09-22T18:56:21Z 2021-01-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10333 https://link.springer.com/article/10.1007/s11356-021-12389-9 0944-1344 1614-7499 (online) https://doi.org/10.1007/s11356-021-12389-9 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Environmental Science and Pollution Research 28: 22843–22852. (2021) |
| spellingShingle | Soybeans Toxicity Abiotic Stress Bioaccumulation Hydroponics Toxicidad Estrés Abiótico Glycine Max Soja Bioacumulación Cultivo Hidropónico Pb Morpho-Physiological Parameters Hydroponic System Blanco, Andrés Pignata, María L. Lascano, Hernan Ramiro Rodriguez, Judith Hebelen Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stages |
| title | Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stages |
| title_full | Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stages |
| title_fullStr | Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stages |
| title_full_unstemmed | Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stages |
| title_short | Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stages |
| title_sort | assessment of lead tolerance on glycine max l merr at early growth stages |
| topic | Soybeans Toxicity Abiotic Stress Bioaccumulation Hydroponics Toxicidad Estrés Abiótico Glycine Max Soja Bioacumulación Cultivo Hidropónico Pb Morpho-Physiological Parameters Hydroponic System |
| url | http://hdl.handle.net/20.500.12123/10333 https://link.springer.com/article/10.1007/s11356-021-12389-9 https://doi.org/10.1007/s11356-021-12389-9 |
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