Intersex and liver alterations induced by long-term sublethal exposure to 17α-ethinylestradiol in adult male Cnesterodon decemmaculatus (Pisces: Poeciliidae)
The aim of the present study was to assess the responses of the gonopodium morphology and the gonadal and liver histology of adult male Cnesterodon decemmaculatus to sublethal long-term exposure concentrations of 17a-ethinylestradiol (EE2). Two experiments were conducted exposing the fish to waterbo...
| Main Authors: | , , , , , |
|---|---|
| Format: | info:eu-repo/semantics/article |
| Language: | Inglés |
| Published: |
2017
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/1033 http://onlinelibrary.wiley.com/doi/10.1002/etc.3547/epdf |
| _version_ | 1855034727589740544 |
|---|---|
| author | Young, Brian Jonathan López, Gabriela Carina Cristos, Diego Sebastian Crespo, Diana Elvira Somoza, Gustavo Manuel Carriquiriborde, Pedro |
| author_browse | Carriquiriborde, Pedro Crespo, Diana Elvira Cristos, Diego Sebastian López, Gabriela Carina Somoza, Gustavo Manuel Young, Brian Jonathan |
| author_facet | Young, Brian Jonathan López, Gabriela Carina Cristos, Diego Sebastian Crespo, Diana Elvira Somoza, Gustavo Manuel Carriquiriborde, Pedro |
| author_sort | Young, Brian Jonathan |
| collection | INTA Digital |
| description | The aim of the present study was to assess the responses of the gonopodium morphology and the gonadal and liver histology of adult male Cnesterodon decemmaculatus to sublethal long-term exposure concentrations of 17a-ethinylestradiol (EE2). Two experiments were conducted exposing the fish to waterborne concentrations of EE2 ranging from 20 ng/L to 200 ng/L for 8 wk, 12 wk,
and 16 wk. Intersex gonads were observed after 8wk and 16 wk in fish exposed to 200 ng EE2/L and 100 ng EE2/L, respectively. Oocytes’ development from testis germ cells and replacement of the efferent duct periodic acid-Schiff–positive secretion surrounding spermatozeugmata by parenchymal tissue and duct structure alterations were the major observed changes in the gonads. In contrast, no response was observed in the gonopodium morphology. Liver histology was also altered, showing increasing steatosis, single-cell
necrosis to generalized necrosis, and disruption of acinar organization from 100 ng EE2/L to 200 ng EE2/L. In summary, the present results showed that although EE2 was not able to alter the morphology of a developed gonopodium, it was capable of inducing development of testicular oocytes in adult male C. decemmaculatus at environmentally relevant concentrations. Thus, externally normal but intersex C. decemmaculatus males would be expected in the wastewater-receiving streams that the species inhabits. According to the
literature, the present study would be the first indicating estrogen-induced intersex in adult male poeciliid. |
| format | info:eu-repo/semantics/article |
| id | INTA1033 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA10332018-02-22T14:13:39Z Intersex and liver alterations induced by long-term sublethal exposure to 17α-ethinylestradiol in adult male Cnesterodon decemmaculatus (Pisces: Poeciliidae) Young, Brian Jonathan López, Gabriela Carina Cristos, Diego Sebastian Crespo, Diana Elvira Somoza, Gustavo Manuel Carriquiriborde, Pedro Poeciliidae Peces Estradiol Toxicidad Fishes Toxicity Cnesterodon Decemmaculatus The aim of the present study was to assess the responses of the gonopodium morphology and the gonadal and liver histology of adult male Cnesterodon decemmaculatus to sublethal long-term exposure concentrations of 17a-ethinylestradiol (EE2). Two experiments were conducted exposing the fish to waterborne concentrations of EE2 ranging from 20 ng/L to 200 ng/L for 8 wk, 12 wk, and 16 wk. Intersex gonads were observed after 8wk and 16 wk in fish exposed to 200 ng EE2/L and 100 ng EE2/L, respectively. Oocytes’ development from testis germ cells and replacement of the efferent duct periodic acid-Schiff–positive secretion surrounding spermatozeugmata by parenchymal tissue and duct structure alterations were the major observed changes in the gonads. In contrast, no response was observed in the gonopodium morphology. Liver histology was also altered, showing increasing steatosis, single-cell necrosis to generalized necrosis, and disruption of acinar organization from 100 ng EE2/L to 200 ng EE2/L. In summary, the present results showed that although EE2 was not able to alter the morphology of a developed gonopodium, it was capable of inducing development of testicular oocytes in adult male C. decemmaculatus at environmentally relevant concentrations. Thus, externally normal but intersex C. decemmaculatus males would be expected in the wastewater-receiving streams that the species inhabits. According to the literature, the present study would be the first indicating estrogen-induced intersex in adult male poeciliid. Inst. de Microbiología y Zoología Agrícola IMyZA Fil: Young, Brian Jonathan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Laboratorio de Transformación de Residuos; Argentina Fil: Lopez, Gabriela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: Cristos, Diego Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos. Laboratorio de Contaminantes Químicos; Argentina Fil: Crespo, Diana Elvira. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Laboratorio de Transformación de Residuos; Argentina Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: Carriquiriborde, Pedro. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata; Argentina 2017-08-24T14:04:47Z 2017-08-24T14:04:47Z 2017 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:ar-repo/semantics/artículo http://hdl.handle.net/20.500.12123/1033 http://onlinelibrary.wiley.com/doi/10.1002/etc.3547/epdf 0730-7268 (Print) 1552-8618 (Online) DOI: 10.1002/etc.3547 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Environmental toxicology and chemistry 36 (7) : 1738–1745. (2017) |
| spellingShingle | Poeciliidae Peces Estradiol Toxicidad Fishes Toxicity Cnesterodon Decemmaculatus Young, Brian Jonathan López, Gabriela Carina Cristos, Diego Sebastian Crespo, Diana Elvira Somoza, Gustavo Manuel Carriquiriborde, Pedro Intersex and liver alterations induced by long-term sublethal exposure to 17α-ethinylestradiol in adult male Cnesterodon decemmaculatus (Pisces: Poeciliidae) |
| title | Intersex and liver alterations induced by long-term sublethal exposure to 17α-ethinylestradiol in adult male Cnesterodon decemmaculatus (Pisces: Poeciliidae) |
| title_full | Intersex and liver alterations induced by long-term sublethal exposure to 17α-ethinylestradiol in adult male Cnesterodon decemmaculatus (Pisces: Poeciliidae) |
| title_fullStr | Intersex and liver alterations induced by long-term sublethal exposure to 17α-ethinylestradiol in adult male Cnesterodon decemmaculatus (Pisces: Poeciliidae) |
| title_full_unstemmed | Intersex and liver alterations induced by long-term sublethal exposure to 17α-ethinylestradiol in adult male Cnesterodon decemmaculatus (Pisces: Poeciliidae) |
| title_short | Intersex and liver alterations induced by long-term sublethal exposure to 17α-ethinylestradiol in adult male Cnesterodon decemmaculatus (Pisces: Poeciliidae) |
| title_sort | intersex and liver alterations induced by long term sublethal exposure to 17α ethinylestradiol in adult male cnesterodon decemmaculatus pisces poeciliidae |
| topic | Poeciliidae Peces Estradiol Toxicidad Fishes Toxicity Cnesterodon Decemmaculatus |
| url | http://hdl.handle.net/20.500.12123/1033 http://onlinelibrary.wiley.com/doi/10.1002/etc.3547/epdf |
| work_keys_str_mv | AT youngbrianjonathan intersexandliveralterationsinducedbylongtermsublethalexposureto17aethinylestradiolinadultmalecnesterodondecemmaculatuspiscespoeciliidae AT lopezgabrielacarina intersexandliveralterationsinducedbylongtermsublethalexposureto17aethinylestradiolinadultmalecnesterodondecemmaculatuspiscespoeciliidae AT cristosdiegosebastian intersexandliveralterationsinducedbylongtermsublethalexposureto17aethinylestradiolinadultmalecnesterodondecemmaculatuspiscespoeciliidae AT crespodianaelvira intersexandliveralterationsinducedbylongtermsublethalexposureto17aethinylestradiolinadultmalecnesterodondecemmaculatuspiscespoeciliidae AT somozagustavomanuel intersexandliveralterationsinducedbylongtermsublethalexposureto17aethinylestradiolinadultmalecnesterodondecemmaculatuspiscespoeciliidae AT carriquiribordepedro intersexandliveralterationsinducedbylongtermsublethalexposureto17aethinylestradiolinadultmalecnesterodondecemmaculatuspiscespoeciliidae |