Atrazine uptake, translocation, bioaccumulation and biodegradation in cattail (Typha latifolia) as a function of exposure time
The extensive use and environmental persistence of atrazine has resulted in its ubiquitous occurrence in water resources. Some reports have described atrazine bioaccumulation and biodegradation pathways in terrestrial plants, but few have done so in aquatic macrophytes. Thus, in this study, we aimed...
| Autores principales: | , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Español |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/10293 https://www.sciencedirect.com/science/article/pii/S0045653521025765 https://doi.org/10.1016/j.chemosphere.2021.132104 |
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| author | Pérez, Débora Jesabel Doucette, William Joseph Moore, Matthew Truman |
| author_browse | Doucette, William Joseph Moore, Matthew Truman Pérez, Débora Jesabel |
| author_facet | Pérez, Débora Jesabel Doucette, William Joseph Moore, Matthew Truman |
| author_sort | Pérez, Débora Jesabel |
| collection | INTA Digital |
| description | The extensive use and environmental persistence of atrazine has resulted in its ubiquitous occurrence in water resources. Some reports have described atrazine bioaccumulation and biodegradation pathways in terrestrial plants, but few have done so in aquatic macrophytes. Thus, in this study, we aimed to analyze morphological changes, uptake, translocation and bioaccumulation patterns in tissues of the aquatic macrophyte Typha latifolia (cattail) after long-term atrazine exposure and to determine the presence of atrazine biodegradation metabolites, desethylatrazine (DEA) and desisopropylatrazine (DIA), in tissues. Plants were hydroponically exposed to 20 μg/L atrazine (18 exposed and 18 non-exposed) for 7, 14, 21, 28, 35 and 42 days. Plants were separated into root, rhizome, stem, and lower, middle and upper leaf sections. Atrazine was analyzed by LC-MS/MS and DIA and DEA by LC-DAD. Plants showed reductions in weight (after 21 days) and transpiration (after 28 days), both symptoms of chronic phytotoxicity. The distribution of atrazine within tissues, expressed as concentration levels (μg/kg dry weight), was as follows: middle leaf (406.10 ± 71.77) = upper leaf (339.15 ± 47.60) = lower leaf (262.43 ± 7.66) = sprout (274.53 ± 58.1) > stem (38.63 ± 7.55) = root (36.00 ± 3.49) = rhizome (26.15 ± 3.96). In submerged tissues, DEA and DIA were detected at similar concentrations. In leaves, DIA was the main metabolite identified. Results indicated that atrazine was taken up from roots to shoots and induced phytotoxicity effects that reduced the translocation to shoots. Typha likely is able to biodegrade atrazine via different metabolic pathways. |
| format | info:ar-repo/semantics/artículo |
| id | INTA10293 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Español |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA102932021-09-20T10:52:13Z Atrazine uptake, translocation, bioaccumulation and biodegradation in cattail (Typha latifolia) as a function of exposure time Pérez, Débora Jesabel Doucette, William Joseph Moore, Matthew Truman Herbicidas Atrazina Metabolitos Bioacumulación Transpiración Fitotoxicidad Herbicides Atrazine Metabolites Bioaccumulation Transpiration Phytotoxicity Typha Typha Latifolia Cattails The extensive use and environmental persistence of atrazine has resulted in its ubiquitous occurrence in water resources. Some reports have described atrazine bioaccumulation and biodegradation pathways in terrestrial plants, but few have done so in aquatic macrophytes. Thus, in this study, we aimed to analyze morphological changes, uptake, translocation and bioaccumulation patterns in tissues of the aquatic macrophyte Typha latifolia (cattail) after long-term atrazine exposure and to determine the presence of atrazine biodegradation metabolites, desethylatrazine (DEA) and desisopropylatrazine (DIA), in tissues. Plants were hydroponically exposed to 20 μg/L atrazine (18 exposed and 18 non-exposed) for 7, 14, 21, 28, 35 and 42 days. Plants were separated into root, rhizome, stem, and lower, middle and upper leaf sections. Atrazine was analyzed by LC-MS/MS and DIA and DEA by LC-DAD. Plants showed reductions in weight (after 21 days) and transpiration (after 28 days), both symptoms of chronic phytotoxicity. The distribution of atrazine within tissues, expressed as concentration levels (μg/kg dry weight), was as follows: middle leaf (406.10 ± 71.77) = upper leaf (339.15 ± 47.60) = lower leaf (262.43 ± 7.66) = sprout (274.53 ± 58.1) > stem (38.63 ± 7.55) = root (36.00 ± 3.49) = rhizome (26.15 ± 3.96). In submerged tissues, DEA and DIA were detected at similar concentrations. In leaves, DIA was the main metabolite identified. Results indicated that atrazine was taken up from roots to shoots and induced phytotoxicity effects that reduced the translocation to shoots. Typha likely is able to biodegrade atrazine via different metabolic pathways. EEA Balcarce Fil: Pérez, Débora Jesabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación Para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Doucette, William Joseph. Utah State University. Utah Water Research Laboratory; Estados Unidos. Fil: Moore, Matthew Truman. United States Department of Agriculture. Water Quality and Ecology Research Unit; Estados Unidos. 2021-09-20T10:44:58Z 2021-09-20T10:44:58Z 2021-08-30 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10293 https://www.sciencedirect.com/science/article/pii/S0045653521025765 0045-6535 https://doi.org/10.1016/j.chemosphere.2021.132104 spa info:eu-repograntAgreement/INTA/2019-PD-E2-I039-002/2019-PD-E2-I039-002/AR./REMEDIACIÓN DE SUELOS Y AGUAS Y RESTAURACIÓN ECOLÓGICA DE SISTEMAS DEGRADADOS POR USO AGROPECUARIO, AGROINDUSTRIAL Y ACTIVIDADES EXTRACTIVAS info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Chemosphere 287, Part 1: 132104 (January 2022) |
| spellingShingle | Herbicidas Atrazina Metabolitos Bioacumulación Transpiración Fitotoxicidad Herbicides Atrazine Metabolites Bioaccumulation Transpiration Phytotoxicity Typha Typha Latifolia Cattails Pérez, Débora Jesabel Doucette, William Joseph Moore, Matthew Truman Atrazine uptake, translocation, bioaccumulation and biodegradation in cattail (Typha latifolia) as a function of exposure time |
| title | Atrazine uptake, translocation, bioaccumulation and biodegradation in cattail (Typha latifolia) as a function of exposure time |
| title_full | Atrazine uptake, translocation, bioaccumulation and biodegradation in cattail (Typha latifolia) as a function of exposure time |
| title_fullStr | Atrazine uptake, translocation, bioaccumulation and biodegradation in cattail (Typha latifolia) as a function of exposure time |
| title_full_unstemmed | Atrazine uptake, translocation, bioaccumulation and biodegradation in cattail (Typha latifolia) as a function of exposure time |
| title_short | Atrazine uptake, translocation, bioaccumulation and biodegradation in cattail (Typha latifolia) as a function of exposure time |
| title_sort | atrazine uptake translocation bioaccumulation and biodegradation in cattail typha latifolia as a function of exposure time |
| topic | Herbicidas Atrazina Metabolitos Bioacumulación Transpiración Fitotoxicidad Herbicides Atrazine Metabolites Bioaccumulation Transpiration Phytotoxicity Typha Typha Latifolia Cattails |
| url | http://hdl.handle.net/20.500.12123/10293 https://www.sciencedirect.com/science/article/pii/S0045653521025765 https://doi.org/10.1016/j.chemosphere.2021.132104 |
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