Detection by multiplex PCR of Mycoplasma species associated with dairy cattle in Argentina = Detección por PCR multiplex de especies de Mycoplasma asociadas a ganado lechero en Argentina
There is scarce information about the frequency and epidemiological and clinical features associated with the presence of Mycoplasma spp. in Argentine dairy herds. The objectives of this study were to develop a multiplex PCR for identifying M. bovis and M. canadense and to describe the frequency of...
| Autores principales: | , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/10256 https://www.sciencedirect.com/science/article/pii/S0325754121000791 https://doi.org/10.1016/j.ram.2021.07.001 |
| _version_ | 1855484555173036032 |
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| author | Neder, Veronica Elizabeth Amadio, Ariel Calvinho, Luis Fernando |
| author_browse | Amadio, Ariel Calvinho, Luis Fernando Neder, Veronica Elizabeth |
| author_facet | Neder, Veronica Elizabeth Amadio, Ariel Calvinho, Luis Fernando |
| author_sort | Neder, Veronica Elizabeth |
| collection | INTA Digital |
| description | There is scarce information about the frequency and epidemiological and clinical features associated with the presence of Mycoplasma spp. in Argentine dairy herds. The objectives of this study were to develop a multiplex PCR for identifying M. bovis and M. canadense and to describe the frequency of Mycoplasma spp. isolated from clinical samples submitted to a diagnostic laboratory. Of a total of 1548 samples from intramammary infections, bulk tank milk and biological fluids, 38 Mycoplasma isolates were obtained. M. bovis, M. canadense, M. californicum and M. leachii were detected by using two multiplex PCRs, confirming their presence in clinical conditions in dairy cattle. The techniques used in the present study can be useful to broaden the knowledge about Mycoplasma infections in cattle, since the search for these organisms is not usually included in routine diagnoses. |
| format | Artículo |
| id | INTA10256 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA102562021-09-15T11:10:58Z Detection by multiplex PCR of Mycoplasma species associated with dairy cattle in Argentina = Detección por PCR multiplex de especies de Mycoplasma asociadas a ganado lechero en Argentina Neder, Veronica Elizabeth Amadio, Ariel Calvinho, Luis Fernando Ganado Bovino Ganado de Leche Mycoplasma PCR Argentina Cattle Dairy Cattle Reacción en Cadena de la Polimerasa Polymerase Chain Reaction There is scarce information about the frequency and epidemiological and clinical features associated with the presence of Mycoplasma spp. in Argentine dairy herds. The objectives of this study were to develop a multiplex PCR for identifying M. bovis and M. canadense and to describe the frequency of Mycoplasma spp. isolated from clinical samples submitted to a diagnostic laboratory. Of a total of 1548 samples from intramammary infections, bulk tank milk and biological fluids, 38 Mycoplasma isolates were obtained. M. bovis, M. canadense, M. californicum and M. leachii were detected by using two multiplex PCRs, confirming their presence in clinical conditions in dairy cattle. The techniques used in the present study can be useful to broaden the knowledge about Mycoplasma infections in cattle, since the search for these organisms is not usually included in routine diagnoses. Existe poca información sobre la frecuencia, así como las características epidemiológicas y clínicas asociadas con la presencia de Mycoplasma en los rodeos lecheros argentinos. Los objetivos de este estudio fueron desarrollar una PCR multiplex para identificar M. bovis y M. canadense y describir la frecuencia de especies de Mycoplasma aisladas de muestras clínicas enviadas a un laboratorio de diagnóstico. De un total de 1.548 muestras de infecciones intramamarias, leche de tanque de frío y fluidos biológicos, se obtuvieron 38 aislamientos de Mycoplasma. Mediante 2 PCR multiplex se detectaron M. bovis, M. canadense, M. californicum y M. leachii, confirmando su presencia en síndromes clínicos en ganado lechero. Las técnicas utilizadas en el presente estudio pueden ser útiles para ampliar el conocimiento sobre las infecciones por Mycoplasma en bovinos, ya que la búsqueda de estos organismos no suele incluirse en los diagnósticos de rutina. EEA Rafaela Fil: Neder, Veronica Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Neder, Veronica Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Amadio, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Calvinho, Luis Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Calvinho, Luis Fernando. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina 2021-09-15T11:05:32Z 2021-09-15T11:05:32Z 2021 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/10256 https://www.sciencedirect.com/science/article/pii/S0325754121000791 0325-7541 1851-7617 https://doi.org/10.1016/j.ram.2021.07.001 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Argentina .......... (nation) (World, South America) 7006477 Elsevier Revista Argentina de Microbiología (Available online 4 September 2021) |
| spellingShingle | Ganado Bovino Ganado de Leche Mycoplasma PCR Argentina Cattle Dairy Cattle Reacción en Cadena de la Polimerasa Polymerase Chain Reaction Neder, Veronica Elizabeth Amadio, Ariel Calvinho, Luis Fernando Detection by multiplex PCR of Mycoplasma species associated with dairy cattle in Argentina = Detección por PCR multiplex de especies de Mycoplasma asociadas a ganado lechero en Argentina |
| title | Detection by multiplex PCR of Mycoplasma species associated with dairy cattle in Argentina = Detección por PCR multiplex de especies de Mycoplasma asociadas a ganado lechero en Argentina |
| title_full | Detection by multiplex PCR of Mycoplasma species associated with dairy cattle in Argentina = Detección por PCR multiplex de especies de Mycoplasma asociadas a ganado lechero en Argentina |
| title_fullStr | Detection by multiplex PCR of Mycoplasma species associated with dairy cattle in Argentina = Detección por PCR multiplex de especies de Mycoplasma asociadas a ganado lechero en Argentina |
| title_full_unstemmed | Detection by multiplex PCR of Mycoplasma species associated with dairy cattle in Argentina = Detección por PCR multiplex de especies de Mycoplasma asociadas a ganado lechero en Argentina |
| title_short | Detection by multiplex PCR of Mycoplasma species associated with dairy cattle in Argentina = Detección por PCR multiplex de especies de Mycoplasma asociadas a ganado lechero en Argentina |
| title_sort | detection by multiplex pcr of mycoplasma species associated with dairy cattle in argentina deteccion por pcr multiplex de especies de mycoplasma asociadas a ganado lechero en argentina |
| topic | Ganado Bovino Ganado de Leche Mycoplasma PCR Argentina Cattle Dairy Cattle Reacción en Cadena de la Polimerasa Polymerase Chain Reaction |
| url | http://hdl.handle.net/20.500.12123/10256 https://www.sciencedirect.com/science/article/pii/S0325754121000791 https://doi.org/10.1016/j.ram.2021.07.001 |
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