Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication
Low arbuscular-mycorrhizal (AM) sporulation in arid field soils limits our knowledge of indigenous species when diversity studies are based only on spore morphology. Our aim was to use different approaches (i.e., spore morphological approach and PCR–SSCP (single-strand-conformation-polymorphism) ana...
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| Format: | Artículo |
| Language: | Inglés |
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Multidisciplinary Digital Publishing Institute (MDPI)
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/10249 https://www.mdpi.com/2223-7747/10/9/1803/html https://doi.org/10.3390/plants10091803 |
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| author | Covacevich, Fernanda Hernandez Guijarro, Keren Crespo, Esteban Lumini, Erica Rivero Mega, María Soledad Lugo, Mónica Alejandra |
| author_browse | Covacevich, Fernanda Crespo, Esteban Hernandez Guijarro, Keren Lugo, Mónica Alejandra Lumini, Erica Rivero Mega, María Soledad |
| author_facet | Covacevich, Fernanda Hernandez Guijarro, Keren Crespo, Esteban Lumini, Erica Rivero Mega, María Soledad Lugo, Mónica Alejandra |
| author_sort | Covacevich, Fernanda |
| collection | INTA Digital |
| description | Low arbuscular-mycorrhizal (AM) sporulation in arid field soils limits our knowledge of indigenous species when diversity studies are based only on spore morphology. Our aim was to use different approaches (i.e., spore morphological approach and PCR–SSCP (single-strand-conformation-polymorphism) analysis after trap plant multiplication strategies to improve the knowledge of the current richness of glomalean AM fungi (Glomerales; Glomeromycota) from the Argentine Puna. Indigenous propagules from two pristine sites at 3870 and 3370 m of elevation were multiplied using different host plants; propagation periods (2–6 months), and subculture cycles (1; 2; or 3) from 5 to 13 months. The propagule multiplication experiment allowed the detection of different glomoid taxa of Funneliformis spp. and Rhizoglomus spp., which were considered cryptic species since they had never been found in Puna soils before. On the other hand; almost all the generalist species previously described were recovered from cultures; except for Glomus ambisporum. Both plant host selection and culture times are critical for Glomerales multiplication. The SSCP analysis complemented the morphological approach and showed a high variability of Glomus at each site; revealing the presence of Funneliformis mosseae. This study demonstrates that AMF trap culture (TC) is a useful strategy for improving the analysis of AM fungal diversity/richness in the Argentinean highlands. |
| format | Artículo |
| id | INTA10249 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Multidisciplinary Digital Publishing Institute (MDPI) |
| publisherStr | Multidisciplinary Digital Publishing Institute (MDPI) |
| record_format | dspace |
| spelling | INTA102492025-01-09T10:12:36Z Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication Covacevich, Fernanda Hernandez Guijarro, Keren Crespo, Esteban Lumini, Erica Rivero Mega, María Soledad Lugo, Mónica Alejandra Suelo Hongos Biodiversidad Micorrizas Arbusculares Propágulos Zona de Montaña Argentina Soil Fungi Biodiversity Arbuscular Mycorrhiza Sets Highlands Low arbuscular-mycorrhizal (AM) sporulation in arid field soils limits our knowledge of indigenous species when diversity studies are based only on spore morphology. Our aim was to use different approaches (i.e., spore morphological approach and PCR–SSCP (single-strand-conformation-polymorphism) analysis after trap plant multiplication strategies to improve the knowledge of the current richness of glomalean AM fungi (Glomerales; Glomeromycota) from the Argentine Puna. Indigenous propagules from two pristine sites at 3870 and 3370 m of elevation were multiplied using different host plants; propagation periods (2–6 months), and subculture cycles (1; 2; or 3) from 5 to 13 months. The propagule multiplication experiment allowed the detection of different glomoid taxa of Funneliformis spp. and Rhizoglomus spp., which were considered cryptic species since they had never been found in Puna soils before. On the other hand; almost all the generalist species previously described were recovered from cultures; except for Glomus ambisporum. Both plant host selection and culture times are critical for Glomerales multiplication. The SSCP analysis complemented the morphological approach and showed a high variability of Glomus at each site; revealing the presence of Funneliformis mosseae. This study demonstrates that AMF trap culture (TC) is a useful strategy for improving the analysis of AM fungal diversity/richness in the Argentinean highlands. EEA Balcarce Fil: Covacevich, Fernanada. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para la Investigación Biológicas Aplicadas. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina. Fil: Hernández Guijarro, Keren. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Crespo, Esteban. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Fil: Lumini, Erica. Institute for Sustainable Plant Protection of National Research Council; Italia. Fil: Rivero Mega, María Soledad. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Fil: Lugo, Mónica. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Fil: Lugo, Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Luis; Argentina. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. 2021-09-14T11:45:50Z 2021-09-14T11:45:50Z 2021-08-30 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10249 https://www.mdpi.com/2223-7747/10/9/1803/html 2223-7747 https://doi.org/10.3390/plants10091803 eng info:eu-repograntAgreement/INTA/2019-PD-E2-I037-002/2019-PD-E2-I037-002/AR./Biodiversidad edáfica: componente clave para una gestión integral y sustentable del recurso suelo info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Multidisciplinary Digital Publishing Institute (MDPI) Plants 10 (9) : 1803 (September 2021) |
| spellingShingle | Suelo Hongos Biodiversidad Micorrizas Arbusculares Propágulos Zona de Montaña Argentina Soil Fungi Biodiversity Arbuscular Mycorrhiza Sets Highlands Covacevich, Fernanda Hernandez Guijarro, Keren Crespo, Esteban Lumini, Erica Rivero Mega, María Soledad Lugo, Mónica Alejandra Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication |
| title | Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication |
| title_full | Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication |
| title_fullStr | Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication |
| title_full_unstemmed | Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication |
| title_short | Arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication |
| title_sort | arbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplication |
| topic | Suelo Hongos Biodiversidad Micorrizas Arbusculares Propágulos Zona de Montaña Argentina Soil Fungi Biodiversity Arbuscular Mycorrhiza Sets Highlands |
| url | http://hdl.handle.net/20.500.12123/10249 https://www.mdpi.com/2223-7747/10/9/1803/html https://doi.org/10.3390/plants10091803 |
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