Diversity structure of the microbial communities in the guts of four neotropical termite species
The termite gut microbiome is dominated by lignocellulose degrading microorganisms. This study describes the intestinal microbiota of four Argentinian higher termite species with different feeding habits: Microcerotermes strunckii (hardwood), Nasutitermes corniger (softwood), Termes riograndensis (s...
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Peer J Inc.
2021
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/10238 https://peerj.com/articles/10959/ https://doi.org/10.7717/peerj.10959 |
| _version_ | 1855036356290412544 |
|---|---|
| author | Vikram, Surendra Arneodo Larochette, Joel Demian Calcagno, Javier Ortiz, Maximiliano Mon, Maria Laura Etcheverry, Clara Cowan, Donald Talia, Paola Mónica |
| author_browse | Arneodo Larochette, Joel Demian Calcagno, Javier Cowan, Donald Etcheverry, Clara Mon, Maria Laura Ortiz, Maximiliano Talia, Paola Mónica Vikram, Surendra |
| author_facet | Vikram, Surendra Arneodo Larochette, Joel Demian Calcagno, Javier Ortiz, Maximiliano Mon, Maria Laura Etcheverry, Clara Cowan, Donald Talia, Paola Mónica |
| author_sort | Vikram, Surendra |
| collection | INTA Digital |
| description | The termite gut microbiome is dominated by lignocellulose degrading microorganisms. This study describes the intestinal microbiota of four Argentinian higher termite species with different feeding habits: Microcerotermes strunckii (hardwood), Nasutitermes corniger (softwood), Termes riograndensis (soil organic matter/grass) and Cornitermes cumulans (grass) by deep sequencing of amplified 16S rRNA and ITS genes. In addition, we have performed a taxonomic and gut community structure comparison incorporating into the analysis the previously reported microbiomes of additional termite species with varied diets. The bacterial phylum Spirochaetes was dominant in the guts of M. strunckii, N. corniger and C. cumulans, whereas Firmicutes predominated in the T. riograndensis gut microbiome. A single bacterial genus, Treponema (Spirochaetes), was dominant in all termite species, except for T. riograndensis. Both in our own sequenced samples and in the broader comparison, prokaryotic α-diversity was higher in the soil/grass feeders than in the wood feeders. Meanwhile, the β-diversity of prokaryotes and fungi was highly dissimilar among strict wood-feeders, whereas that of soil- and grass-feeders grouped more closely. Ascomycota and Basidiomycota were the only fungal phyla that could be identified in all gut samples, because of the lack of reference sequences in public databases. In summary, higher microbial diversity was recorded in termites with more versatile feeding sources, providing further evidence that diet, along with other factors (e.g., host taxonomy), influences the microbial community assembly in the termite gut. |
| format | info:ar-repo/semantics/artículo |
| id | INTA10238 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Peer J Inc. |
| publisherStr | Peer J Inc. |
| record_format | dspace |
| spelling | INTA102382021-09-13T14:22:54Z Diversity structure of the microbial communities in the guts of four neotropical termite species Vikram, Surendra Arneodo Larochette, Joel Demian Calcagno, Javier Ortiz, Maximiliano Mon, Maria Laura Etcheverry, Clara Cowan, Donald Talia, Paola Mónica Isoptera Flora Microbiana Termitidae Microbial Flora Prokaryotae Microbiota The termite gut microbiome is dominated by lignocellulose degrading microorganisms. This study describes the intestinal microbiota of four Argentinian higher termite species with different feeding habits: Microcerotermes strunckii (hardwood), Nasutitermes corniger (softwood), Termes riograndensis (soil organic matter/grass) and Cornitermes cumulans (grass) by deep sequencing of amplified 16S rRNA and ITS genes. In addition, we have performed a taxonomic and gut community structure comparison incorporating into the analysis the previously reported microbiomes of additional termite species with varied diets. The bacterial phylum Spirochaetes was dominant in the guts of M. strunckii, N. corniger and C. cumulans, whereas Firmicutes predominated in the T. riograndensis gut microbiome. A single bacterial genus, Treponema (Spirochaetes), was dominant in all termite species, except for T. riograndensis. Both in our own sequenced samples and in the broader comparison, prokaryotic α-diversity was higher in the soil/grass feeders than in the wood feeders. Meanwhile, the β-diversity of prokaryotes and fungi was highly dissimilar among strict wood-feeders, whereas that of soil- and grass-feeders grouped more closely. Ascomycota and Basidiomycota were the only fungal phyla that could be identified in all gut samples, because of the lack of reference sequences in public databases. In summary, higher microbial diversity was recorded in termites with more versatile feeding sources, providing further evidence that diet, along with other factors (e.g., host taxonomy), influences the microbial community assembly in the termite gut. Instituto de Biotecnología Fil: Vikram, Surendra. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfrica Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Arneodo Larochette, Joel Demian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Calcagno, Javier. Universidad Maimonides. Centro de Ciencias Naturales, Ambientales y Antropológicas; Argentina Fil: Ortiz, Maximiliano. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfrica Fil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Mon, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Etcheverry, Clara. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Biología de los Invertebrados; Argentina Fil: Cowan, Donald. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfrica Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Talia, Paola Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-09-13T14:14:46Z 2021-09-13T14:14:46Z 2021-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10238 https://peerj.com/articles/10959/ 2167-8359 https://doi.org/10.7717/peerj.10959 eng info:eu-repograntAgreement/INTA/PNAIyAV-1130034/AR./Desarrollo de procesos para la transformación de biomasa en bioenergía. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Peer J Inc. PeerJ. 9 : e10959 (Abril 2021) |
| spellingShingle | Isoptera Flora Microbiana Termitidae Microbial Flora Prokaryotae Microbiota Vikram, Surendra Arneodo Larochette, Joel Demian Calcagno, Javier Ortiz, Maximiliano Mon, Maria Laura Etcheverry, Clara Cowan, Donald Talia, Paola Mónica Diversity structure of the microbial communities in the guts of four neotropical termite species |
| title | Diversity structure of the microbial communities in the guts of four neotropical termite species |
| title_full | Diversity structure of the microbial communities in the guts of four neotropical termite species |
| title_fullStr | Diversity structure of the microbial communities in the guts of four neotropical termite species |
| title_full_unstemmed | Diversity structure of the microbial communities in the guts of four neotropical termite species |
| title_short | Diversity structure of the microbial communities in the guts of four neotropical termite species |
| title_sort | diversity structure of the microbial communities in the guts of four neotropical termite species |
| topic | Isoptera Flora Microbiana Termitidae Microbial Flora Prokaryotae Microbiota |
| url | http://hdl.handle.net/20.500.12123/10238 https://peerj.com/articles/10959/ https://doi.org/10.7717/peerj.10959 |
| work_keys_str_mv | AT vikramsurendra diversitystructureofthemicrobialcommunitiesinthegutsoffourneotropicaltermitespecies AT arneodolarochettejoeldemian diversitystructureofthemicrobialcommunitiesinthegutsoffourneotropicaltermitespecies AT calcagnojavier diversitystructureofthemicrobialcommunitiesinthegutsoffourneotropicaltermitespecies AT ortizmaximiliano diversitystructureofthemicrobialcommunitiesinthegutsoffourneotropicaltermitespecies AT monmarialaura diversitystructureofthemicrobialcommunitiesinthegutsoffourneotropicaltermitespecies AT etcheverryclara diversitystructureofthemicrobialcommunitiesinthegutsoffourneotropicaltermitespecies AT cowandonald diversitystructureofthemicrobialcommunitiesinthegutsoffourneotropicaltermitespecies AT taliapaolamonica diversitystructureofthemicrobialcommunitiesinthegutsoffourneotropicaltermitespecies |