Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora
This is the first report describing the genetic transformation of Diaporthe caulivora, the soybean stem canker fungus. A simple and 100% efficient protocol of Agrobacterium tumefaciens-mediated transformation used mycelium as starting material and the hygromycin B resistance and green fluorescent pr...
| Main Authors: | , , , |
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| Format: | Artículo |
| Language: | Español |
| Published: |
Elsevier
2021
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/10234 https://www.sciencedirect.com/science/article/pii/S0167701221000658 https://doi.org/10.1016/j.mimet.2021.106197 |
| _version_ | 1855484551350976512 |
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| author | Montoya, Marina Rosa Massa, Gabriela Alejandra Colabelli, Mabel Noemí Ridao, Azucena |
| author_browse | Colabelli, Mabel Noemí Massa, Gabriela Alejandra Montoya, Marina Rosa Ridao, Azucena |
| author_facet | Montoya, Marina Rosa Massa, Gabriela Alejandra Colabelli, Mabel Noemí Ridao, Azucena |
| author_sort | Montoya, Marina Rosa |
| collection | INTA Digital |
| description | This is the first report describing the genetic transformation of Diaporthe caulivora, the soybean stem canker fungus. A simple and 100% efficient protocol of Agrobacterium tumefaciens-mediated transformation used mycelium as starting material and the hygromycin B resistance and green fluorescent protein (GFP) as a selection and reporter agents, respectively. All transgenic isolates were mitotically stable in two independent experiments and polymerase chain reaction with hygromycin B resistance primers confirmed successful T-DNA integration into the fungal genome. Plant-fungus interaction studies, including pathogenicity, latency, and endophytism, as well as further studies of random and targeted mutagenesis will be possible with GFP-expressing isolates of D. caulivora and other species in the Diaporthe / Phomopsis complex. |
| format | Artículo |
| id | INTA10234 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Español |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA102342021-09-13T13:23:26Z Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora Montoya, Marina Rosa Massa, Gabriela Alejandra Colabelli, Mabel Noemí Ridao, Azucena Soja Tallos Diaporthe Agrobacterium Tumefaciens Infecciones Latentes Soybeans Stems Latent Infection Diaporthe caulivora This is the first report describing the genetic transformation of Diaporthe caulivora, the soybean stem canker fungus. A simple and 100% efficient protocol of Agrobacterium tumefaciens-mediated transformation used mycelium as starting material and the hygromycin B resistance and green fluorescent protein (GFP) as a selection and reporter agents, respectively. All transgenic isolates were mitotically stable in two independent experiments and polymerase chain reaction with hygromycin B resistance primers confirmed successful T-DNA integration into the fungal genome. Plant-fungus interaction studies, including pathogenicity, latency, and endophytism, as well as further studies of random and targeted mutagenesis will be possible with GFP-expressing isolates of D. caulivora and other species in the Diaporthe / Phomopsis complex. EEA Balcarce Fil: Montoya, Marina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Massa. Gabriela Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Massa. Gabriela Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Colabelli, Mabel Noemí. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Ridao, Azucena del Carmen. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. 2021-09-13T13:16:34Z 2021-09-13T13:16:34Z 2021-03-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10234 https://www.sciencedirect.com/science/article/pii/S0167701221000658 0167-7012 https://doi.org/10.1016/j.mimet.2021.106197 spa info:eu-repograntAgreement/INTA/PNPV-1135024/AR./Defensa en plantas contra fitopatógenos. info:eu-repograntAgreement/INTA/PNCYO-1127034/AR./Evaluación y desarrollo de sistemas de manejo integrado de las plagas en cultivos de cereales y oleaginosas. info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Journal of Microbiological Methods 184 : 106197 (May 2021) |
| spellingShingle | Soja Tallos Diaporthe Agrobacterium Tumefaciens Infecciones Latentes Soybeans Stems Latent Infection Diaporthe caulivora Montoya, Marina Rosa Massa, Gabriela Alejandra Colabelli, Mabel Noemí Ridao, Azucena Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora |
| title | Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora |
| title_full | Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora |
| title_fullStr | Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora |
| title_full_unstemmed | Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora |
| title_short | Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora |
| title_sort | efficient agrobacterium tumefaciens mediated transformation system of diaporthe caulivora |
| topic | Soja Tallos Diaporthe Agrobacterium Tumefaciens Infecciones Latentes Soybeans Stems Latent Infection Diaporthe caulivora |
| url | http://hdl.handle.net/20.500.12123/10234 https://www.sciencedirect.com/science/article/pii/S0167701221000658 https://doi.org/10.1016/j.mimet.2021.106197 |
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