Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull
Campylobacter fetus is a well-recognized pathogen that affects reproductive rate in cattle. In the present study, two Angus bulls were kept (39 days) separately with a group of heifers experimentally infected withCampylobacter fetus subsp.venerealis (Cfv) andCampylobacter fetus subsp.venerealis bio...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer Verlag
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/10233 http://link.springer.com/10.1007/s11259-021-09823-1 https://doi.org/10.1007/s11259-021-09823-1 |
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| author | García, Juan Agustín Gioffre, Andrea Acuña, Juaquín Mendez, Maria Alejandra Morsella, Claudia Graciela Aller Atucha, Juan Florencio Paolicchi, Fernando |
| author_browse | Acuña, Juaquín Aller Atucha, Juan Florencio García, Juan Agustín Gioffre, Andrea Mendez, Maria Alejandra Morsella, Claudia Graciela Paolicchi, Fernando |
| author_facet | García, Juan Agustín Gioffre, Andrea Acuña, Juaquín Mendez, Maria Alejandra Morsella, Claudia Graciela Aller Atucha, Juan Florencio Paolicchi, Fernando |
| author_sort | García, Juan Agustín |
| collection | INTA Digital |
| description | Campylobacter fetus is a well-recognized pathogen that affects reproductive rate in cattle. In the present study, two Angus bulls were kept (39 days) separately with a group of heifers experimentally infected withCampylobacter fetus subsp.venerealis (Cfv) andCampylobacter fetus subsp.venerealis biovarintermedius (Cfvi), respectively. Each bull resulted infected post-mating by its respective strain (Cfv andCfvi). Semen samples collected from each bull at days 39, 82, 132 and 269 resulted positive forC. fetus by bacteriological culture and/or direct immunofluorescence (DIF) test, and confirmed by polymerase chain reaction (PCR) from colonies isolated. Diagnosis resulted better with bacteriological culture (100%) compared to DIF (37,5%).Campylobacter fetus was isolated from seminal vesicle and preputial mucosa by bacteriological culture and confirmed by PCR and DIF test from colonies previously isolated from these tissues (day 276). Microscopic lesions detected in both bulls showed moderate diffuse subepithelial lymphoplasmacytic postitis. None of the seminal vesicle presented relevant microscopic lesions. To our knowledge this is the first report of isolation ofC. fetus from seminal vesicles in a bull. The experimental model herein described, mimicks the natural infection and constitutes a promising alternative for future studies of campylobacteriosis in cattle. |
| format | info:ar-repo/semantics/artículo |
| id | INTA10233 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer Verlag |
| publisherStr | Springer Verlag |
| record_format | dspace |
| spelling | INTA102332021-09-13T13:14:08Z Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull García, Juan Agustín Gioffre, Andrea Acuña, Juaquín Mendez, Maria Alejandra Morsella, Claudia Graciela Aller Atucha, Juan Florencio Paolicchi, Fernando Bovinae Rumiante Enfermedad de Transmición Sexual Campilobacteriosis Glándulas Vesiculares Ruminants Sexually Transmitted Diseases Campylobacteriosis Vesicular Glands Campylobacter fetus is a well-recognized pathogen that affects reproductive rate in cattle. In the present study, two Angus bulls were kept (39 days) separately with a group of heifers experimentally infected withCampylobacter fetus subsp.venerealis (Cfv) andCampylobacter fetus subsp.venerealis biovarintermedius (Cfvi), respectively. Each bull resulted infected post-mating by its respective strain (Cfv andCfvi). Semen samples collected from each bull at days 39, 82, 132 and 269 resulted positive forC. fetus by bacteriological culture and/or direct immunofluorescence (DIF) test, and confirmed by polymerase chain reaction (PCR) from colonies isolated. Diagnosis resulted better with bacteriological culture (100%) compared to DIF (37,5%).Campylobacter fetus was isolated from seminal vesicle and preputial mucosa by bacteriological culture and confirmed by PCR and DIF test from colonies previously isolated from these tissues (day 276). Microscopic lesions detected in both bulls showed moderate diffuse subepithelial lymphoplasmacytic postitis. None of the seminal vesicle presented relevant microscopic lesions. To our knowledge this is the first report of isolation ofC. fetus from seminal vesicles in a bull. The experimental model herein described, mimicks the natural infection and constitutes a promising alternative for future studies of campylobacteriosis in cattle. EEA Balcarce Fil: García, Juan Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: García, Juan Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Gioffré, Andrea K. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Acuña, Joaquín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Méndez, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Paolicchi, Fernando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. 2021-09-13T13:06:06Z 2021-09-13T13:06:06Z 2021-09-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10233 http://link.springer.com/10.1007/s11259-021-09823-1 1573-7446 (on line) 0165-7380 (print) https://doi.org/10.1007/s11259-021-09823-1 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I103-001/2019-PD-E5-I103-001/AR./Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud pública info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001/2019-PD-E5-I105-001/AR./Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco “Una Salud” info:eu-repo/semantics/restrictedAccess application/pdf Springer Verlag Veterinary science communications (september 2021) |
| spellingShingle | Bovinae Rumiante Enfermedad de Transmición Sexual Campilobacteriosis Glándulas Vesiculares Ruminants Sexually Transmitted Diseases Campylobacteriosis Vesicular Glands García, Juan Agustín Gioffre, Andrea Acuña, Juaquín Mendez, Maria Alejandra Morsella, Claudia Graciela Aller Atucha, Juan Florencio Paolicchi, Fernando Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull |
| title | Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull |
| title_full | Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull |
| title_fullStr | Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull |
| title_full_unstemmed | Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull |
| title_short | Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull |
| title_sort | isolation of campylobacter fetus subsp venerealis from seminal vesicle of a naturally challenged bull |
| topic | Bovinae Rumiante Enfermedad de Transmición Sexual Campilobacteriosis Glándulas Vesiculares Ruminants Sexually Transmitted Diseases Campylobacteriosis Vesicular Glands |
| url | http://hdl.handle.net/20.500.12123/10233 http://link.springer.com/10.1007/s11259-021-09823-1 https://doi.org/10.1007/s11259-021-09823-1 |
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