A proposed role for endomembrane trafficking processes in regulating tonoplast content and vacuole dynamics under ammonium stress conditions in Arabidopsis root cells
Ammonium (NH4+) stress has multiple effects on plant physiology, therefore, plant responses are complex, and multiple mechanisms are involved in NH4+ sensitivity and tolerance in plants. Root growth inhibition is an important quantitative readout of the effects of NH4+ stress on plant physiology, an...
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| Format: | Artículo |
| Language: | Inglés |
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Taylor and Francis
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/10225 https://www.tandfonline.com/doi/abs/10.1080/15592324.2021.1924977 https://doi.org/10.1080/15592324.2021.1924977 |
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| author | Robert, German Yagyu, Mako Lascano, Hernan Ramiro Masclaux-Daubresse, Céline Yoshimoto, Kohki |
| author_browse | Lascano, Hernan Ramiro Masclaux-Daubresse, Céline Robert, German Yagyu, Mako Yoshimoto, Kohki |
| author_facet | Robert, German Yagyu, Mako Lascano, Hernan Ramiro Masclaux-Daubresse, Céline Yoshimoto, Kohki |
| author_sort | Robert, German |
| collection | INTA Digital |
| description | Ammonium (NH4+) stress has multiple effects on plant physiology, therefore, plant responses are complex, and multiple mechanisms are involved in NH4+ sensitivity and tolerance in plants. Root growth inhibition is an important quantitative readout of the effects of NH4+ stress on plant physiology, and cell elongation appear as the principal growth inhibition target. We recently proposed autophagy as a relevant physiological mechanisms underlying NH4+ sensitivity response in Arabidopsis. In a brief overview, the impaired macro-autophagic flux observed under NH4+ stress conditions has a detrimental impact on the cellular energetic balance, and therefore on the energy-demanding plant growth. In contrast to its inhibitory effect on the autophagosomes flux to vacuole, NH4+ toxicity induced a micro-autophagy-like process. Consistent with the reduced membrane flux to the vacuole related to macro-autophagy inhibition and the increased tonoplast degradation due to enhanced micro-autophagy, the vacuoles of the root cells of the NH4+-stressed plants showed lower tonoplast content and a decreased perimeter/area ratio. As the endosome-to-vacuole trafficking is another important process that contributes to membrane flux toward the vacuole, we evaluated the effects of NH4+ stress on this process. This allows us to propose that autophagy could contribute to vacuole development as well as possible avenues to follow for future studies. |
| format | Artículo |
| id | INTA10225 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Taylor and Francis |
| publisherStr | Taylor and Francis |
| record_format | dspace |
| spelling | INTA102252021-09-10T12:28:39Z A proposed role for endomembrane trafficking processes in regulating tonoplast content and vacuole dynamics under ammonium stress conditions in Arabidopsis root cells Robert, German Yagyu, Mako Lascano, Hernan Ramiro Masclaux-Daubresse, Céline Yoshimoto, Kohki Endocytosis Vacuoles Morphology Cell Elongation Arabidopsis Endocitosis Vacúola Morfología Elongación Celular Ammonium Toxicity Endomembrane Trafficking Macroautophagy Microautophagy Ammonium (NH4+) stress has multiple effects on plant physiology, therefore, plant responses are complex, and multiple mechanisms are involved in NH4+ sensitivity and tolerance in plants. Root growth inhibition is an important quantitative readout of the effects of NH4+ stress on plant physiology, and cell elongation appear as the principal growth inhibition target. We recently proposed autophagy as a relevant physiological mechanisms underlying NH4+ sensitivity response in Arabidopsis. In a brief overview, the impaired macro-autophagic flux observed under NH4+ stress conditions has a detrimental impact on the cellular energetic balance, and therefore on the energy-demanding plant growth. In contrast to its inhibitory effect on the autophagosomes flux to vacuole, NH4+ toxicity induced a micro-autophagy-like process. Consistent with the reduced membrane flux to the vacuole related to macro-autophagy inhibition and the increased tonoplast degradation due to enhanced micro-autophagy, the vacuoles of the root cells of the NH4+-stressed plants showed lower tonoplast content and a decreased perimeter/area ratio. As the endosome-to-vacuole trafficking is another important process that contributes to membrane flux toward the vacuole, we evaluated the effects of NH4+ stress on this process. This allows us to propose that autophagy could contribute to vacuole development as well as possible avenues to follow for future studies. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Robert, German. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Robert, German. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Yagyu, Mako. Meiji University. School of Agriculture. Department of Life Sciences; Japón Fil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales. Argentina Fil: Masclaux-Daubresse, Céline. Université Paris-Saclay. AgroParisTech. Institut Jean-Pierre Bourgin. Francia Fil: Yoshimoto, Kohki. Meiji University. School of Agriculture. Department of Life Sciences; Japón 2021-09-10T12:20:05Z 2021-09-10T12:20:05Z 2021-05-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10225 https://www.tandfonline.com/doi/abs/10.1080/15592324.2021.1924977 1559-2316 1559-2324 (online) https://doi.org/10.1080/15592324.2021.1924977 eng info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001/2019-PD-E6-I116-001/AR./Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial. info:eu-repo/semantics/restrictedAccess application/pdf Taylor and Francis Plant Signaling & Behavior 16 (9): e1924977 (2021) |
| spellingShingle | Endocytosis Vacuoles Morphology Cell Elongation Arabidopsis Endocitosis Vacúola Morfología Elongación Celular Ammonium Toxicity Endomembrane Trafficking Macroautophagy Microautophagy Robert, German Yagyu, Mako Lascano, Hernan Ramiro Masclaux-Daubresse, Céline Yoshimoto, Kohki A proposed role for endomembrane trafficking processes in regulating tonoplast content and vacuole dynamics under ammonium stress conditions in Arabidopsis root cells |
| title | A proposed role for endomembrane trafficking processes in regulating tonoplast content and vacuole dynamics under ammonium stress conditions in Arabidopsis root cells |
| title_full | A proposed role for endomembrane trafficking processes in regulating tonoplast content and vacuole dynamics under ammonium stress conditions in Arabidopsis root cells |
| title_fullStr | A proposed role for endomembrane trafficking processes in regulating tonoplast content and vacuole dynamics under ammonium stress conditions in Arabidopsis root cells |
| title_full_unstemmed | A proposed role for endomembrane trafficking processes in regulating tonoplast content and vacuole dynamics under ammonium stress conditions in Arabidopsis root cells |
| title_short | A proposed role for endomembrane trafficking processes in regulating tonoplast content and vacuole dynamics under ammonium stress conditions in Arabidopsis root cells |
| title_sort | proposed role for endomembrane trafficking processes in regulating tonoplast content and vacuole dynamics under ammonium stress conditions in arabidopsis root cells |
| topic | Endocytosis Vacuoles Morphology Cell Elongation Arabidopsis Endocitosis Vacúola Morfología Elongación Celular Ammonium Toxicity Endomembrane Trafficking Macroautophagy Microautophagy |
| url | http://hdl.handle.net/20.500.12123/10225 https://www.tandfonline.com/doi/abs/10.1080/15592324.2021.1924977 https://doi.org/10.1080/15592324.2021.1924977 |
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