The role of glucose in the pathology of EHEC O157: H7
The pathogen enterohemorrhagic Escherichia coli (EHEC) O157: H7 is responsible for hemorrhagic colitis and hemolytic uremic syndrome in humans [1]. During the colonization process in the gastrointestinal tract, EHEC needs to adapt to changes in nutrient availability [2]. The objective of this stu...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Cambridge University Press
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/10224 https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/role-of-glucose-in-the-pathology-of-ehec-o157-h7/4F5327F46D17EE9933438979E0134B28 https://doi.org/10.1017/S1431927620001129 |
| _version_ | 1855036354421850112 |
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| author | Marques Da Silva, Wanderson Taibo, Catalina Beatriz Sabio Y Garcia, Julia Veronica Larzabal, Mariano Cataldi, Angel Adrian |
| author_browse | Cataldi, Angel Adrian Larzabal, Mariano Marques Da Silva, Wanderson Sabio Y Garcia, Julia Veronica Taibo, Catalina Beatriz |
| author_facet | Marques Da Silva, Wanderson Taibo, Catalina Beatriz Sabio Y Garcia, Julia Veronica Larzabal, Mariano Cataldi, Angel Adrian |
| author_sort | Marques Da Silva, Wanderson |
| collection | INTA Digital |
| description | The pathogen enterohemorrhagic Escherichia coli (EHEC) O157: H7 is responsible for hemorrhagic
colitis and hemolytic uremic syndrome in humans [1]. During the colonization process in the
gastrointestinal tract, EHEC needs to adapt to changes in nutrient availability [2]. The objective of this
study was to evaluate the influence of glucose on physiology and processes involved in the pathogenesis
of EHEC O157: H7 in order to improve our understanding of the mechanisms controlling EHEC growth
and survival in the bovine gut.
In this study we first analyzed the growth rate of EHEC O157: H7 Rafaela II clade 8, a strain isolated
from a bovine in Argentina, grown in the medium DMEM supplemented with either 4.5% glucose (Highglucose - DHG) or 1% glucose (Low-glucose - DLG). In addition, we assessed the bacterial adhesion
capacity and actin pedestal formation induced by EHEC [3] by performing infection assays. For this
purpose, Caco-2 epithelial cells were exposed for 5 h with Rafaela II grown with the different
concentrations of glucose. Subsequently, the samples were fixed (paraformaldehyde 4%) and
permeabilized (triton); actin and nucleic acids (DNA) were stained with rhodamine-phalloidin and TOPRO-3, respectively. Bacterial adhesion capacity and pedestal formation of cells were evaluated using a
Leica TCS SP5 laser scanning confocal microscope (MC). Each Image was acquired by monitoring a
single focal plane over time (xyt scanning mode) using a 40X/1.25 oil objective lens and 543nm HeNe
and 633 nm HeNe lasers. The frequency and resolution for acquiring images were set at 200 Hz and 1,024
x 1,024 pixels, while maintaining the same settings for laser powers, gain, and offset. |
| format | info:ar-repo/semantics/artículo |
| id | INTA10224 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | INTA102242021-09-10T12:16:49Z The role of glucose in the pathology of EHEC O157: H7 Marques Da Silva, Wanderson Taibo, Catalina Beatriz Sabio Y Garcia, Julia Veronica Larzabal, Mariano Cataldi, Angel Adrian Glucose Pathogens Pathogenesis Glucosa Organismos Patógenos Escherichia coli Patogénesis The pathogen enterohemorrhagic Escherichia coli (EHEC) O157: H7 is responsible for hemorrhagic colitis and hemolytic uremic syndrome in humans [1]. During the colonization process in the gastrointestinal tract, EHEC needs to adapt to changes in nutrient availability [2]. The objective of this study was to evaluate the influence of glucose on physiology and processes involved in the pathogenesis of EHEC O157: H7 in order to improve our understanding of the mechanisms controlling EHEC growth and survival in the bovine gut. In this study we first analyzed the growth rate of EHEC O157: H7 Rafaela II clade 8, a strain isolated from a bovine in Argentina, grown in the medium DMEM supplemented with either 4.5% glucose (Highglucose - DHG) or 1% glucose (Low-glucose - DLG). In addition, we assessed the bacterial adhesion capacity and actin pedestal formation induced by EHEC [3] by performing infection assays. For this purpose, Caco-2 epithelial cells were exposed for 5 h with Rafaela II grown with the different concentrations of glucose. Subsequently, the samples were fixed (paraformaldehyde 4%) and permeabilized (triton); actin and nucleic acids (DNA) were stained with rhodamine-phalloidin and TOPRO-3, respectively. Bacterial adhesion capacity and pedestal formation of cells were evaluated using a Leica TCS SP5 laser scanning confocal microscope (MC). Each Image was acquired by monitoring a single focal plane over time (xyt scanning mode) using a 40X/1.25 oil objective lens and 543nm HeNe and 633 nm HeNe lasers. The frequency and resolution for acquiring images were set at 200 Hz and 1,024 x 1,024 pixels, while maintaining the same settings for laser powers, gain, and offset. Instituto de Biotecnología Fil: Marques Da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Marques Da Silva, Wanderson. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Taibo, Catalina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; Argentina Fil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Sabio Y Garcia, Julia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; Argentina Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-09-10T12:12:36Z 2021-09-10T12:12:36Z 2020-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10224 https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/role-of-glucose-in-the-pathology-of-ehec-o157-h7/4F5327F46D17EE9933438979E0134B28 1431-9276 https://doi.org/10.1017/S1431927620001129 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Cambridge University Press Microscopy and Microanalysis 26 (Suppl 1) : 181-182 (Abril 2020) |
| spellingShingle | Glucose Pathogens Pathogenesis Glucosa Organismos Patógenos Escherichia coli Patogénesis Marques Da Silva, Wanderson Taibo, Catalina Beatriz Sabio Y Garcia, Julia Veronica Larzabal, Mariano Cataldi, Angel Adrian The role of glucose in the pathology of EHEC O157: H7 |
| title | The role of glucose in the pathology of EHEC O157: H7 |
| title_full | The role of glucose in the pathology of EHEC O157: H7 |
| title_fullStr | The role of glucose in the pathology of EHEC O157: H7 |
| title_full_unstemmed | The role of glucose in the pathology of EHEC O157: H7 |
| title_short | The role of glucose in the pathology of EHEC O157: H7 |
| title_sort | role of glucose in the pathology of ehec o157 h7 |
| topic | Glucose Pathogens Pathogenesis Glucosa Organismos Patógenos Escherichia coli Patogénesis |
| url | http://hdl.handle.net/20.500.12123/10224 https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/role-of-glucose-in-the-pathology-of-ehec-o157-h7/4F5327F46D17EE9933438979E0134B28 https://doi.org/10.1017/S1431927620001129 |
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