Potential of rhizobacteria native to Argentina for the control of Meloidogyne javanica.
Biocontrol of the nematode Meloidogyne javanica was studied using the Argentinean strains Pseudomonas fluorescens MME3, TAE4, TAR5 and ZME4 and Bacillus sp. B7S, B9T and B19S. Pseudomonas protegens CHA0 was used as a positive control. Egg hatching and juvenile mortality were evaluated in vitro by ex...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Asociación Argentina de Microbiología
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/10144 https://www.sciencedirect.com/science/article/pii/S0325754121000456 https://doi.org/10.1016/j.ram.2021.02.010 |
| _version_ | 1855484534085124096 |
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| author | Borrajo, Paula Mondino, Eduardo Ariel Maroniche, Guillermo Andrés Fernández, Macarena Creus, Cecilia |
| author_browse | Borrajo, Paula Creus, Cecilia Fernández, Macarena Maroniche, Guillermo Andrés Mondino, Eduardo Ariel |
| author_facet | Borrajo, Paula Mondino, Eduardo Ariel Maroniche, Guillermo Andrés Fernández, Macarena Creus, Cecilia |
| author_sort | Borrajo, Paula |
| collection | INTA Digital |
| description | Biocontrol of the nematode Meloidogyne javanica was studied using the Argentinean strains Pseudomonas fluorescens MME3, TAE4, TAR5 and ZME4 and Bacillus sp. B7S, B9T and B19S. Pseudomonas protegens CHA0 was used as a positive control. Egg hatching and juvenile mortality were evaluated in vitro by exposure of nematodes to bacterial suspensions or their cell-free supernatants (CFS). The effect of bacteria on nematode infestation of lettuce was also studied. results showed that most of the tested strains and CFS reduced egg hatching and juvenile survival in vitro. The bacterial suspension of Bacillus sp. B9T produced the lowest hatching of eggs. Juvenile mortality was higher when M. javanica was exposed to Bacillus sp. than to Pseudomonas spp. suspensions. Except for CFS of B9T, all filtrates inhibited hatching at levels similar to or higher than the biocontrol strain P. protegens CHA0. The CFS of CHA0 showed the highest level of juvenile mortality followed by Bacillus sp. strains and P. fluorescens TAE4. None of the inoculated rhizobacteria reverted the negative effect of infestation on the aerial dry weight of lettuce plants. However, inoculation impacted on reproduction of M. javanica by reducing the development of galls and egg masses on roots and diminishing the number of individuals both on roots and in the substrate, as well as the reproduction factor. These results show that most of the analyzed native strains can control the nematode M. javanica. Among them, P. fluorescens TAE4 and Bacillus sp. B9T showed the most promising performances for the biocontrol of this pathogen and have a potential use in the formulation of commercial products. |
| format | Artículo |
| id | INTA10144 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Asociación Argentina de Microbiología |
| publisherStr | Asociación Argentina de Microbiología |
| record_format | dspace |
| spelling | INTA101442021-08-30T14:08:43Z Potential of rhizobacteria native to Argentina for the control of Meloidogyne javanica. Potencial de rizobacterias nativas de Argentina para el control de Meloidogyne javanica. Borrajo, Paula Mondino, Eduardo Ariel Maroniche, Guillermo Andrés Fernández, Macarena Creus, Cecilia Bacillus Pseudomonas Fluorescens Control Biológico Nematodo de las Plantas Lechugas Argentina Biological Control Plant Nematodes Lettuces Biocontrol of the nematode Meloidogyne javanica was studied using the Argentinean strains Pseudomonas fluorescens MME3, TAE4, TAR5 and ZME4 and Bacillus sp. B7S, B9T and B19S. Pseudomonas protegens CHA0 was used as a positive control. Egg hatching and juvenile mortality were evaluated in vitro by exposure of nematodes to bacterial suspensions or their cell-free supernatants (CFS). The effect of bacteria on nematode infestation of lettuce was also studied. results showed that most of the tested strains and CFS reduced egg hatching and juvenile survival in vitro. The bacterial suspension of Bacillus sp. B9T produced the lowest hatching of eggs. Juvenile mortality was higher when M. javanica was exposed to Bacillus sp. than to Pseudomonas spp. suspensions. Except for CFS of B9T, all filtrates inhibited hatching at levels similar to or higher than the biocontrol strain P. protegens CHA0. The CFS of CHA0 showed the highest level of juvenile mortality followed by Bacillus sp. strains and P. fluorescens TAE4. None of the inoculated rhizobacteria reverted the negative effect of infestation on the aerial dry weight of lettuce plants. However, inoculation impacted on reproduction of M. javanica by reducing the development of galls and egg masses on roots and diminishing the number of individuals both on roots and in the substrate, as well as the reproduction factor. These results show that most of the analyzed native strains can control the nematode M. javanica. Among them, P. fluorescens TAE4 and Bacillus sp. B9T showed the most promising performances for the biocontrol of this pathogen and have a potential use in the formulation of commercial products. Se estudiaron las cepas argentinas Pseudomonas fluorescens MME3, TAE4, TAR5 y ZME4 y Bacillus sp. B7S, B9T y B19S para el control del nematodo Meloidogyne javanica. Pseudomonas protegens CHA0 se utilizó como control positivo. La eclosión de huevos y la mortalidad de juveniles se evaluaron in vitro al exponerlos a suspensiones bacterianas y a sus sobrenadantes libres de células (SLC). Asimismo, se estudió la inoculación bacteriana sobre la infestación del nematodo en lechuga. Los resultados in vitro indicaron que la mayoría de las cepas, así como sus SLC redujeron la eclosión y la supervivencia de M. javanica. La suspensión de Bacillus sp. B9T produjo los menores niveles de eclosión. La mortalidad de juveniles fue mayor al exponerlos a suspensiones de Bacillus sp. respecto de Pseudomonas spp. Los SLC inhibieron la eclosión de huevos en niveles similares o superiores a P. protegens CHA0, excepto por el de B9T. La exposición a SLC de CHA0 registró la mayor mortalidad, seguido por las cepas de Bacillus sp. y P. fluorescens TAE4. La inoculación bacteriana no revertió el efecto de la infestación sobre el peso seco aéreo de las plantas, sin embargo, afectó la multiplicación de M. javanica lo que redujo el desarrollo de agallas y las masas de huevos, y disminuyó el número de individuos presentes tanto en la raíz como en el sustrato, así como el factor de reproducción. Los resultados indican que la mayoría de las cepas nativas evaluadas son capaces de controlar a M. javanica. Entre ellas, P. fluorescens TAE4 y Bacillus sp. B9T, se presentan como las más promisorias para el control de este patógeno, con potencialidad para ser utilizadas en la formulación de productos biológicos. EEA Balcarce Fil: Borrajo, María Paula. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Borrajo, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Mondino, Eduardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Fernández, Macarena. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Fernández, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Creus, Cecilia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. 2021-08-30T13:54:41Z 2021-08-30T13:54:41Z 2021-05-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/10144 https://www.sciencedirect.com/science/article/pii/S0325754121000456 0325-7541 https://doi.org/10.1016/j.ram.2021.02.010 eng info:eu-repograntAgreement/INTA/2019-PD-E4-I069-001/2019-PD-E4-I069-001/AR./Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetal info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Argentina .......... (nation) (World, South America) 7006477 Asociación Argentina de Microbiología Revista Argentina de Microbiología (Available online 2 May 2021) |
| spellingShingle | Bacillus Pseudomonas Fluorescens Control Biológico Nematodo de las Plantas Lechugas Argentina Biological Control Plant Nematodes Lettuces Borrajo, Paula Mondino, Eduardo Ariel Maroniche, Guillermo Andrés Fernández, Macarena Creus, Cecilia Potential of rhizobacteria native to Argentina for the control of Meloidogyne javanica. |
| title | Potential of rhizobacteria native to Argentina for the control of Meloidogyne javanica. |
| title_full | Potential of rhizobacteria native to Argentina for the control of Meloidogyne javanica. |
| title_fullStr | Potential of rhizobacteria native to Argentina for the control of Meloidogyne javanica. |
| title_full_unstemmed | Potential of rhizobacteria native to Argentina for the control of Meloidogyne javanica. |
| title_short | Potential of rhizobacteria native to Argentina for the control of Meloidogyne javanica. |
| title_sort | potential of rhizobacteria native to argentina for the control of meloidogyne javanica |
| topic | Bacillus Pseudomonas Fluorescens Control Biológico Nematodo de las Plantas Lechugas Argentina Biological Control Plant Nematodes Lettuces |
| url | http://hdl.handle.net/20.500.12123/10144 https://www.sciencedirect.com/science/article/pii/S0325754121000456 https://doi.org/10.1016/j.ram.2021.02.010 |
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