Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing.
Plasmopara halstedii (Farl.) Berl. & De Toni causing downy mildew of sunflower is responsible for considerable economic losses worldwide. Because P. halstedii can be seed-transmitted, monitoring of seeds for pathogen contamination is important for the sunflower seed trade. The relevance of asymptoma...
| Autores principales: | , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2021
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/10141 https://www.sciencedirect.com/science/article/pii/S1658077X21000503 https://doi.org/10.1016/j.jssas.2021.04.007 |
| _version_ | 1855036339179749376 |
|---|---|
| author | Martínez, Ana Laura Quiroz, Facundo Jose Carrera, Alicia Delia |
| author_browse | Carrera, Alicia Delia Martínez, Ana Laura Quiroz, Facundo Jose |
| author_facet | Martínez, Ana Laura Quiroz, Facundo Jose Carrera, Alicia Delia |
| author_sort | Martínez, Ana Laura |
| collection | INTA Digital |
| description | Plasmopara halstedii (Farl.) Berl. & De Toni causing downy mildew of sunflower is responsible for considerable economic losses worldwide. Because P. halstedii can be seed-transmitted, monitoring of seeds for pathogen contamination is important for the sunflower seed trade. The relevance of asymptomatic or latent infections as factors of disease spread have not been studied by molecular techniques. A molecular marker based on a putative effector gene of P. halstedii was used to examine the pathogen’s presence in asymptomatic sunflowers growing near patches of mildewed plants in naturally infected fields. The method based on conventional PCR was highly sensitive for detection of P. halstedii in DNA from whole seeds. By the application of this protocol, we found that all seed samples obtained from symptomatic plants amplified the expected fragment, whereas the diagnostic marker identified the presence of pathogen in one out of 21 asymptomatic plants. Possible uses of this marker to detect downy mildew in seed from asymptomatic plants or for race identification are discussed. |
| format | info:ar-repo/semantics/artículo |
| id | INTA10141 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA101412021-08-30T13:23:11Z Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing. Martínez, Ana Laura Quiroz, Facundo Jose Carrera, Alicia Delia Helianthus Annuus Semilla de Girasol Plasmopara Halstedii Mildiu PCR Marcadores Genéticos Enfermedades Fungosas Sunflower Seed Genetic Markers Fungal Diseases Girasol Sunflower Plasmopara halstedii (Farl.) Berl. & De Toni causing downy mildew of sunflower is responsible for considerable economic losses worldwide. Because P. halstedii can be seed-transmitted, monitoring of seeds for pathogen contamination is important for the sunflower seed trade. The relevance of asymptomatic or latent infections as factors of disease spread have not been studied by molecular techniques. A molecular marker based on a putative effector gene of P. halstedii was used to examine the pathogen’s presence in asymptomatic sunflowers growing near patches of mildewed plants in naturally infected fields. The method based on conventional PCR was highly sensitive for detection of P. halstedii in DNA from whole seeds. By the application of this protocol, we found that all seed samples obtained from symptomatic plants amplified the expected fragment, whereas the diagnostic marker identified the presence of pathogen in one out of 21 asymptomatic plants. Possible uses of this marker to detect downy mildew in seed from asymptomatic plants or for race identification are discussed. EEA Balcarce Fil: Martínez, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Fil: Quiroz, Facundo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Carrera, Alicia Delia. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. 2021-08-30T13:13:36Z 2021-08-30T13:13:36Z 2021-05-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/10141 https://www.sciencedirect.com/science/article/pii/S1658077X21000503 1658-077X https://doi.org/10.1016/j.jssas.2021.04.007 eng info:eu-repograntAgreement/INTA/2019-PD-E4-I090-001/2019-PD-E4-I090-001/AR./Análisis de patosistemas en cultivos agrícolas y especies forestales. Caracterización de sus componentes info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Journal of the Saudi Society of Agricultural Sciences (Available online 6 May 2021) |
| spellingShingle | Helianthus Annuus Semilla de Girasol Plasmopara Halstedii Mildiu PCR Marcadores Genéticos Enfermedades Fungosas Sunflower Seed Genetic Markers Fungal Diseases Girasol Sunflower Martínez, Ana Laura Quiroz, Facundo Jose Carrera, Alicia Delia Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing. |
| title | Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing. |
| title_full | Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing. |
| title_fullStr | Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing. |
| title_full_unstemmed | Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing. |
| title_short | Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing. |
| title_sort | detection of plasmopara halstedii in sunflower seeds a case study using molecular testing |
| topic | Helianthus Annuus Semilla de Girasol Plasmopara Halstedii Mildiu PCR Marcadores Genéticos Enfermedades Fungosas Sunflower Seed Genetic Markers Fungal Diseases Girasol Sunflower |
| url | http://hdl.handle.net/20.500.12123/10141 https://www.sciencedirect.com/science/article/pii/S1658077X21000503 https://doi.org/10.1016/j.jssas.2021.04.007 |
| work_keys_str_mv | AT martinezanalaura detectionofplasmoparahalstediiinsunflowerseedsacasestudyusingmoleculartesting AT quirozfacundojose detectionofplasmoparahalstediiinsunflowerseedsacasestudyusingmoleculartesting AT carreraaliciadelia detectionofplasmoparahalstediiinsunflowerseedsacasestudyusingmoleculartesting |