The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato
Brassinosteroids (BRs) are steroid hormones that play key roles in plant development and defense. Our goal is to harness the extensive knowledge of the Arabidopsis BR signaling network to improve productivity in crop species. This first requires identifying components of the conserved network and th...
| Main Authors: | , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Oxford University Press
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/10138 https://academic.oup.com/jxb/article-abstract/72/4/1181/5937226 https://doi.org/10.1093/jxb/eraa495 |
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| author | Mori, Kentaro Lemaire-Chamley, Martine Jorly, Joana Carrari, Fernando Conte, Mariana Asamizu, Erika Mizoguchi, Tsuyoshi Ezura, Hiroshi Rothan, Christophe |
| author_browse | Asamizu, Erika Carrari, Fernando Conte, Mariana Ezura, Hiroshi Jorly, Joana Lemaire-Chamley, Martine Mizoguchi, Tsuyoshi Mori, Kentaro Rothan, Christophe |
| author_facet | Mori, Kentaro Lemaire-Chamley, Martine Jorly, Joana Carrari, Fernando Conte, Mariana Asamizu, Erika Mizoguchi, Tsuyoshi Ezura, Hiroshi Rothan, Christophe |
| author_sort | Mori, Kentaro |
| collection | INTA Digital |
| description | Brassinosteroids (BRs) are steroid hormones that play key roles in plant development and defense. Our goal is to harness the extensive knowledge of the Arabidopsis BR signaling network to improve productivity in crop species. This first requires identifying components of the conserved network and their function in the target species. Here, we investigated the function of SlBIM1a, the closest tomato homolog of AtBIM1, which is highly expressed in fruit. SlBIM1a-overexpressing lines displayed severe plant and fruit dwarfism, and histological characterization of different transgenic lines revealed that SlBIM1a expression negatively correlated with fruit pericarp cell size, resulting in fruit size modifications. These growth phenotypes were in contrast to those found in Arabidopsis, and this was confirmed by the reciprocal ectopic expression of SlBIM1a/b in Arabidopsis and of AtBIM1 in tomato. These results determined that BIM1 function depends more on the recipient species than on its primary sequence. Yeast two-hybrid interaction studies and transcriptomic analyses of SlBIM1a-overexpressing fruit further suggested that SlBIM1a acts through its interaction with SlBZH1 to govern the transcriptional regulation of growth-related BR target genes. Together, these results suggest that SlBIM1a is a negative regulator of pericarp cell expansion, possibly at the crossroads with auxin and light signaling. |
| format | Artículo |
| id | INTA10138 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | INTA101382021-08-30T11:41:52Z The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato Mori, Kentaro Lemaire-Chamley, Martine Jorly, Joana Carrari, Fernando Conte, Mariana Asamizu, Erika Mizoguchi, Tsuyoshi Ezura, Hiroshi Rothan, Christophe Arabidopsis thaliana Brasinoesteroides Fruto Crecimiento Solanum lycopersicum Transcripción Brassinosteroids Fruit Growth Transcription Tomatoes Cell Expansion Tomate Expansión Celular Brassinosteroids (BRs) are steroid hormones that play key roles in plant development and defense. Our goal is to harness the extensive knowledge of the Arabidopsis BR signaling network to improve productivity in crop species. This first requires identifying components of the conserved network and their function in the target species. Here, we investigated the function of SlBIM1a, the closest tomato homolog of AtBIM1, which is highly expressed in fruit. SlBIM1a-overexpressing lines displayed severe plant and fruit dwarfism, and histological characterization of different transgenic lines revealed that SlBIM1a expression negatively correlated with fruit pericarp cell size, resulting in fruit size modifications. These growth phenotypes were in contrast to those found in Arabidopsis, and this was confirmed by the reciprocal ectopic expression of SlBIM1a/b in Arabidopsis and of AtBIM1 in tomato. These results determined that BIM1 function depends more on the recipient species than on its primary sequence. Yeast two-hybrid interaction studies and transcriptomic analyses of SlBIM1a-overexpressing fruit further suggested that SlBIM1a acts through its interaction with SlBZH1 to govern the transcriptional regulation of growth-related BR target genes. Together, these results suggest that SlBIM1a is a negative regulator of pericarp cell expansion, possibly at the crossroads with auxin and light signaling. Instituto de Biotecnología Fil: Mori, Kentaro. Institut National de la Recherche Agronomique; Francia Fil: Lemaire-Chamley, Martine. Institut National de la Recherche Agronomique; Francia Fil: Jorly, Joana. Institut National de la Recherche Agronomique; Francia Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Carrari, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Conte, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Conte, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Asamizu, Erika. Ryukoku University. Faculty of Agriculture. Department of Plant Life Science; Japón Fil: Mizoguchi, Tsuyoshi. International Christian University. Department of Natural Sciences; Japón Fil: Ezura, Hiroshi. University of Tsukuba. Tsukuba Plant Innovation Research Center. Faculty of Life and Environmental Sciences; Japón Fil: Rothan, Christophe. Institut National de la Recherche Agronomique; Francia 2021-08-30T11:34:32Z 2021-08-30T11:34:32Z 2021-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10138 https://academic.oup.com/jxb/article-abstract/72/4/1181/5937226 1460-2431 https://doi.org/10.1093/jxb/eraa495 eng info:eu-repo/semantics/restrictedAccess application/pdf Oxford University Press Journal of Experimental Botany 72 (4) : 1181-1197 (Febrero 2021) |
| spellingShingle | Arabidopsis thaliana Brasinoesteroides Fruto Crecimiento Solanum lycopersicum Transcripción Brassinosteroids Fruit Growth Transcription Tomatoes Cell Expansion Tomate Expansión Celular Mori, Kentaro Lemaire-Chamley, Martine Jorly, Joana Carrari, Fernando Conte, Mariana Asamizu, Erika Mizoguchi, Tsuyoshi Ezura, Hiroshi Rothan, Christophe The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato |
| title | The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato |
| title_full | The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato |
| title_fullStr | The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato |
| title_full_unstemmed | The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato |
| title_short | The conserved brassinosteroid-related transcription factor BIM1a negatively regulates fruit growth in tomato |
| title_sort | conserved brassinosteroid related transcription factor bim1a negatively regulates fruit growth in tomato |
| topic | Arabidopsis thaliana Brasinoesteroides Fruto Crecimiento Solanum lycopersicum Transcripción Brassinosteroids Fruit Growth Transcription Tomatoes Cell Expansion Tomate Expansión Celular |
| url | http://hdl.handle.net/20.500.12123/10138 https://academic.oup.com/jxb/article-abstract/72/4/1181/5937226 https://doi.org/10.1093/jxb/eraa495 |
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