An isopentenyl transferase transgenic wheat isoline exhibits less seminal root growth impairment and a differential metabolite profile under Cd stress

Cadmium is one of the most important contaminants and it induces severe plant growth restriction. In this study, we analyzed the metabolic changes associated with root growth restriction caused by cadmium in the early seminal root apex of wheat. Our study included two genotypes: the commercial varie...

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Main Authors: Gomez Mansur, Nabila M., Pena, Liliana Beatriz, Bossio, Adrian Ezequiel, Lewi, Dalia Marcela, Beznec, Ailin, Blumwald, Eduardo, Arbona, Vicent, Gómez-Cadenas, Aurelio, Benavides, María Patricia, Gallego, Susana Mabel
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Wiley 2021
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/10113
https://onlinelibrary.wiley.com/doi/10.1111/ppl.13366
https://doi.org/10.1111/ppl.13366
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author Gomez Mansur, Nabila M.
Pena, Liliana Beatriz
Bossio, Adrian Ezequiel
Lewi, Dalia Marcela
Beznec, Ailin
Blumwald, Eduardo
Arbona, Vicent
Gómez-Cadenas, Aurelio
Benavides, María Patricia
Gallego, Susana Mabel
author_browse Arbona, Vicent
Benavides, María Patricia
Beznec, Ailin
Blumwald, Eduardo
Bossio, Adrian Ezequiel
Gallego, Susana Mabel
Gomez Mansur, Nabila M.
Gómez-Cadenas, Aurelio
Lewi, Dalia Marcela
Pena, Liliana Beatriz
author_facet Gomez Mansur, Nabila M.
Pena, Liliana Beatriz
Bossio, Adrian Ezequiel
Lewi, Dalia Marcela
Beznec, Ailin
Blumwald, Eduardo
Arbona, Vicent
Gómez-Cadenas, Aurelio
Benavides, María Patricia
Gallego, Susana Mabel
author_sort Gomez Mansur, Nabila M.
collection INTA Digital
description Cadmium is one of the most important contaminants and it induces severe plant growth restriction. In this study, we analyzed the metabolic changes associated with root growth restriction caused by cadmium in the early seminal root apex of wheat. Our study included two genotypes: the commercial variety ProINTA Federal (WT) and the PSARK::IPT (IPT) line which exhibit high-grade yield performance under water deficit. Root tips of seedlings grown for 72 h without or with 10 μM CdCl2 (Cd-WT and Cd-IPT) were compared. Root length reduction was more severe in Cd-WT than Cd-IPT. Cd decreased superoxide dismutase activity in both lines and increased catalase activity only in the WT. In Cd-IPT, ascorbate and guaiacol peroxidase activities raised compared to Cd-WT. The hormonal homeostasis was altered by the metal, with significant decreases in abscisic acid, jasmonic acid, 12-oxophytodienoic acid, gibberellins GA20, and GA7 levels. Increases in flavonoids and phenylamides were also found. Root growth impairment was not associated with a decrease in expansin (EXP) transcripts. On the contrary, TaEXPB8 expression increased in the WT treated by Cd. Our findings suggest that the line expressing the PSARK::IPT construction increased the homeostatic range to cope with Cd stress, which is visible by a lesser reduction of the root elongation compared to WT plants. The decline of root growth produced by Cd was associated with hormonal imbalance at the root apex level. We hypothesize that activation of phenolic secondary metabolism could enhance antioxidant defenses and contribute to cell wall reinforcement to deal with Cd toxicity.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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publishDate 2021
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spelling INTA101132021-08-25T12:10:15Z An isopentenyl transferase transgenic wheat isoline exhibits less seminal root growth impairment and a differential metabolite profile under Cd stress Gomez Mansur, Nabila M. Pena, Liliana Beatriz Bossio, Adrian Ezequiel Lewi, Dalia Marcela Beznec, Ailin Blumwald, Eduardo Arbona, Vicent Gómez-Cadenas, Aurelio Benavides, María Patricia Gallego, Susana Mabel Wheat Metabolites Stress Cadmium Genotypes Water Deprivation Roots Transferases Transgenic Plants Trigo Triticum Metabolitos Estres Cadmio Genotipos Privación del Agua Raíces Transferasas Plantas transgénicas Cadmium is one of the most important contaminants and it induces severe plant growth restriction. In this study, we analyzed the metabolic changes associated with root growth restriction caused by cadmium in the early seminal root apex of wheat. Our study included two genotypes: the commercial variety ProINTA Federal (WT) and the PSARK::IPT (IPT) line which exhibit high-grade yield performance under water deficit. Root tips of seedlings grown for 72 h without or with 10 μM CdCl2 (Cd-WT and Cd-IPT) were compared. Root length reduction was more severe in Cd-WT than Cd-IPT. Cd decreased superoxide dismutase activity in both lines and increased catalase activity only in the WT. In Cd-IPT, ascorbate and guaiacol peroxidase activities raised compared to Cd-WT. The hormonal homeostasis was altered by the metal, with significant decreases in abscisic acid, jasmonic acid, 12-oxophytodienoic acid, gibberellins GA20, and GA7 levels. Increases in flavonoids and phenylamides were also found. Root growth impairment was not associated with a decrease in expansin (EXP) transcripts. On the contrary, TaEXPB8 expression increased in the WT treated by Cd. Our findings suggest that the line expressing the PSARK::IPT construction increased the homeostatic range to cope with Cd stress, which is visible by a lesser reduction of the root elongation compared to WT plants. The decline of root growth produced by Cd was associated with hormonal imbalance at the root apex level. We hypothesize that activation of phenolic secondary metabolism could enhance antioxidant defenses and contribute to cell wall reinforcement to deal with Cd toxicity. Instituto de Genética Fil: Gomez Mansur, Nabila M. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Gomez Mansur, Nabila M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pena, Liliana Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Pena, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Blumwald, Eduardo. University of California. Department of Plant Sciences; Estados Unidos Fil: Arbona, Vicent. Universitat Jaume I. Departament de Ciències Agràries i del Medi Natural, Ecofisiologia i Biotecnologia; España Fil: Gómez-Cadenas, Aurelio. Universitat Jaume I. Departament de Ciències Agràries i del Medi Natural, Ecofisiologia i Biotecnologia; España Fil: Benavides, María Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Benavides, María Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gallego, Susana Mabel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Gallego, Susana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-08-25T11:59:20Z 2021-08-25T11:59:20Z 2021-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10113 https://onlinelibrary.wiley.com/doi/10.1111/ppl.13366 1399-3054 https://doi.org/10.1111/ppl.13366 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Physiologia Plantarum 173 (1) : 223-234 (Septiembre 2021)
spellingShingle Wheat
Metabolites
Stress
Cadmium
Genotypes
Water Deprivation
Roots
Transferases
Transgenic Plants
Trigo
Triticum
Metabolitos
Estres
Cadmio
Genotipos
Privación del Agua
Raíces
Transferasas
Plantas transgénicas
Gomez Mansur, Nabila M.
Pena, Liliana Beatriz
Bossio, Adrian Ezequiel
Lewi, Dalia Marcela
Beznec, Ailin
Blumwald, Eduardo
Arbona, Vicent
Gómez-Cadenas, Aurelio
Benavides, María Patricia
Gallego, Susana Mabel
An isopentenyl transferase transgenic wheat isoline exhibits less seminal root growth impairment and a differential metabolite profile under Cd stress
title An isopentenyl transferase transgenic wheat isoline exhibits less seminal root growth impairment and a differential metabolite profile under Cd stress
title_full An isopentenyl transferase transgenic wheat isoline exhibits less seminal root growth impairment and a differential metabolite profile under Cd stress
title_fullStr An isopentenyl transferase transgenic wheat isoline exhibits less seminal root growth impairment and a differential metabolite profile under Cd stress
title_full_unstemmed An isopentenyl transferase transgenic wheat isoline exhibits less seminal root growth impairment and a differential metabolite profile under Cd stress
title_short An isopentenyl transferase transgenic wheat isoline exhibits less seminal root growth impairment and a differential metabolite profile under Cd stress
title_sort isopentenyl transferase transgenic wheat isoline exhibits less seminal root growth impairment and a differential metabolite profile under cd stress
topic Wheat
Metabolites
Stress
Cadmium
Genotypes
Water Deprivation
Roots
Transferases
Transgenic Plants
Trigo
Triticum
Metabolitos
Estres
Cadmio
Genotipos
Privación del Agua
Raíces
Transferasas
Plantas transgénicas
url http://hdl.handle.net/20.500.12123/10113
https://onlinelibrary.wiley.com/doi/10.1111/ppl.13366
https://doi.org/10.1111/ppl.13366
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